Analyses of Transgenic Fibroblast Growth Factor 21 Mature Rice Seeds

Overview

Journal Breed Sci

Date 2019 Sep 5

PMID 31481837

Authors

Mingfang Feng

Hua Cai

Ying Guan

Jian Sun

Liguo Zhang

Jing Cang

Affiliations

Soon will be listed here.

Abstract

Although some studies have been conducted on the effects of foreign protein expression on rice, the results vary with foreign gene types and protein expression. This study reveals the effects of fibroblast growth factor 21 (FGF21) expression on mature rice seeds in various aspects. Results revealed that the grain weight of the transgene rice was lower than that of non-transgenic wild-type. The sucrose content and ADP-glucose pyrophosphorylase (AGPase) activity in transgenic rice were higher than that in non-transgenic wild-type rice, while changes in the starch content, starch branching enzyme (SBE), sucrose synthase (SuS), superoxide dismutase (SOD) and peroxidase (POD) activity were lower in transgenic rice compared to non-transgenic wild-type. The scanning electron microscope results revealed that mature seeds of the transgenic rice contained fewer vascular bundles with irregular arrangement compared to the wild-type. The mature seeds of CK and T1 rice lines were collected for proteome analysis, and 167 differentially expressed proteins (DEPs) were found. In addition, the most enriched pathways in both rice lines were determined to be amino sugar and nucleotide sugar metabolism and starch and sucrose metabolism, This study laid the foundation for revealing the effects of exogenous protein expression on rice bioreactors.

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