Identification and Characterization of the MADS-box Genes Highly Expressed in the Laticifer Cells of Hevea Brasiliensis

Overview

Journal Sci Rep

Specialty Science

Date 2019 Sep 5

PMID 31481699

Citations 1

Authors

Ying Wang

Di-Feng Zhan

Hui-Liang Li

Dong Guo

Jia-Hong Zhu

Shi-Qing Peng

Affiliations

Soon will be listed here.

Abstract

MADS-box transcription factors possess many functions in plant reproduction and development. However, few MADS-box genes related to secondary metabolites regulation have been identified. In Hevea brasiliensis, natural rubber is a representative cis-polyisoprenoids in secondary metabolism which occurs in the rubber laticifer cells, the molecular regulation basis of natural rubber biosynthesis is not clear. Here, a total of 24 MADS-box genes including 4 type I MADS-box genes and 20 type II MADS-box genes were identified in the transcriptome of rubber tree latex. The phylogenetic analysis was performed to clarify the evolutionary relationships of all the 24 rubber tree MADS-box proteins with MADS-box transcription factors from Arabidopsis thaliana and Oryza sativa. Four type I MADS-box genes were subdivided into Mα (3 genes) and Mβ (1 gene). Twenty type II MADS-box genes were subclassified into MIKC* (8 genes) and MIKC (12 genes). Eight MADS-box genes (HblMADS3, 5, 6, 7, 9, 13, 23, 24) were predominant expression in laticifers. ABA up-regulated the expression of HblMADS9, and the expression of HblMADS3, HblMADS5, HblMADS24 were up-regulated by MeJA. The function of HblMADS24 was elucidated. HblMADS24 bound HbFPS1 promoter in yeast and HblMADS24 activated HbFPS1 promoter in tobacco plants. Moreover, we proposed that HblMADS24 is a transcription activator of HbFPS1 which taking part in natural rubber biosynthesis.

Citing Articles

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Genome-Wide Identification of MADS-Box Genes in and : Evolutionary Mechanisms, Conserved Functions and New Functions Related to Natural Rubber Yield Formation.

Chen J, Yang Y, Li C, Chen Q, Liu S, Qin B Int J Mol Sci. 2023; 24(13).

PMID: 37446175 PMC: 10341699. DOI: 10.3390/ijms241310997.

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