Terbium Functionalized Schizochytrium-Derived Carbon Dots for Ratiometric Fluorescence Determination of the Anthrax Biomarker

Overview

Journal Nanomaterials (Basel)

Date 2019 Sep 5

PMID 31480320

Citations 2

Authors

Lina Zhang

Zhanwei Wang

Jingbo Zhang

Changliang Shi

Xiaoli Sun

Dan Zhao

Baozhong Liu

Affiliations

Soon will be listed here.

Abstract

Efficient and instant detection of biological threat-agent anthrax is highly desired in the fields of medical care and anti-terrorism. Herein, a new ratiometric fluorescence (FL) nanoprobe was elaborately tailored for the determination of 2,6-dipicolinic acid (DPA), a biomarker of anthrax spores, by grafting terbium ions (Tb) to the surface of carbon dots (CDs). CDs with blue FL were fabricated by a simple and green method using schizochytrium as precursor and served as an FL reference and a supporting substrate for coordination with Tb. On account of the absorbance energy transfer emission effect (AETE), green emission peaks of Tb in CDs-Tb nanoprobe appeared at 545 nm upon the addition of DPA. Under optimal conditions, good linearity between the ratio FL intensity of / and the concentrations of DPA was observed within the experimental concentration range of 0.5-6 μM with the detection limit of 35.9 nM, which is superior to several literature studies and significantly lower than the infectious dosage of the spores. Moreover, the CDs-Tb nanoprobe could sensitively detect DPA in the lake water sample. This work offers an efficient self-calibrating and background-free method for the determination of DPA.

Citing Articles

Special Issue: Carbon-Based Nanomaterials for (Bio)Sensors Development.

Morais S Nanomaterials (Basel). 2021; 11(9).

PMID: 34578746 PMC: 8469388. DOI: 10.3390/nano11092430.

Quantum Dots as Biosensors in the Determination of Biochemical Parameters in Xenobiotic Exposure and Toxins.

Ravi P, Ganesan M Anal Sci. 2021; 37(5):661-671.

PMID: 33390416 DOI: 10.2116/analsci.20SCR03.

References

Chen H, Xie Y, Kirillov A, Liu L, Yu M, Liu W . A ratiometric fluorescent nanoprobe based on terbium functionalized carbon dots for highly sensitive detection of an anthrax biomarker. Chem Commun (Camb). 2015; 51(24):5036-9. DOI: 10.1039/c5cc00757g. View

Tan C, Wang Q, Zhang C . Optical and electrochemical responses of an anthrax biomarker based on single-walled carbon nanotubes covalently loaded with terbium complexes. Chem Commun (Camb). 2011; 47(46):12521-3. DOI: 10.1039/c1cc15857k. View

Bao L, Liu C, Zhang Z, Pang D . Photoluminescence-tunable carbon nanodots: surface-state energy-gap tuning. Adv Mater. 2015; 27(10):1663-7. DOI: 10.1002/adma.201405070. View

Cable M, Kirby J, Levine D, Manary M, Gray H, Ponce A . Detection of bacterial spores with lanthanide-macrocycle binary complexes. J Am Chem Soc. 2009; 131(27):9562-70. PMC: 2722938. DOI: 10.1021/ja902291v. View

Rong M, Deng X, Chi S, Huang L, Zhou Y, Shen Y . Ratiometric fluorometric determination of the anthrax biomarker 2,6-dipicolinic acid by using europium(III)-doped carbon dots in a test stripe. Mikrochim Acta. 2018; 185(3):201. DOI: 10.1007/s00604-018-2741-6. View

Guo L, Zhang Y, Li W . Sustainable microalgae for the simultaneous synthesis of carbon quantum dots for cellular imaging and porous carbon for CO capture. J Colloid Interface Sci. 2017; 493:257-264. DOI: 10.1016/j.jcis.2017.01.003. View

Ding H, Wei J, Zhong N, Gao Q, Xiong H . Highly Efficient Red-Emitting Carbon Dots with Gram-Scale Yield for Bioimaging. Langmuir. 2017; 33(44):12635-12642. DOI: 10.1021/acs.langmuir.7b02385. View

Zhang X, Zhao J, Whitney A, Elam J, Van Duyne R . Ultrastable substrates for surface-enhanced Raman spectroscopy: Al2O3 overlayers fabricated by atomic layer deposition yield improved anthrax biomarker detection. J Am Chem Soc. 2006; 128(31):10304-9. DOI: 10.1021/ja0638760. View

Zhou Z, Gu J, Chen Y, Zhang X, Wu H, Qiao X . Europium functionalized silicon quantum dots nanomaterials for ratiometric fluorescence detection of Bacillus anthrax biomarker. Spectrochim Acta A Mol Biomol Spectrosc. 2019; 212:88-93. DOI: 10.1016/j.saa.2018.12.036. View

10.

Cable M, Kirby J, Sorasaenee K, Gray H, Ponce A . Bacterial spore detection by [Tb3+(macrocycle)(dipicolinate)] luminescence. J Am Chem Soc. 2007; 129(6):1474-5. DOI: 10.1021/ja061831t. View

11.

Gao N, Zhang Y, Huang P, Xiang Z, Wu F, Mao L . Perturbing Tandem Energy Transfer in Luminescent Heterobinuclear Lanthanide Coordination Polymer Nanoparticles Enables Real-Time Monitoring of Release of the Anthrax Biomarker from Bacterial Spores. Anal Chem. 2018; 90(11):7004-7011. DOI: 10.1021/acs.analchem.8b01365. View

12.

Ma B, Zeng F, Zheng F, Wu S . Fluorescent detection of an anthrax biomarker based on PVA film. Analyst. 2011; 136(18):3649-55. DOI: 10.1039/c1an15384f. View

13.

Luan K, Meng R, Shan C, Cao J, Jia J, Liu W . Terbium Functionalized Micelle Nanoprobe for Ratiometric Fluorescence Detection of Anthrax Spore Biomarker. Anal Chem. 2018; 90(5):3600-3607. DOI: 10.1021/acs.analchem.8b00050. View

14.

Liu M, Chen B, He J, Li C, Li Y, Huang C . Anthrax biomarker: An ultrasensitive fluorescent ratiometry of dipicolinic acid by using terbium(III)-modified carbon dots. Talanta. 2018; 191:443-448. DOI: 10.1016/j.talanta.2018.08.071. View

15.

Zhang L, Wang Z, Zhang J, Jia J, Zhao D, Fan Y . Phenanthroline-Derivative Functionalized Carbon Dots for Highly Selective and Sensitive Detection of Cu and S and Imaging inside Live Cells. Nanomaterials (Basel). 2018; 8(12). PMC: 6315650. DOI: 10.3390/nano8121071. View

16.

Namdari P, Negahdari B, Eatemadi A . Synthesis, properties and biomedical applications of carbon-based quantum dots: An updated review. Biomed Pharmacother. 2017; 87:209-222. DOI: 10.1016/j.biopha.2016.12.108. View

17.

Lee I, Oh W, Jang J . Screen-printed fluorescent sensors for rapid and sensitive anthrax biomarker detection. J Hazard Mater. 2013; 252-253:186-91. DOI: 10.1016/j.jhazmat.2013.03.003. View

18.

Zhang Y, Li B, Ma H, Zhang L, Zheng Y . Rapid and facile ratiometric detection of an anthrax biomarker by regulating energy transfer process in bio-metal-organic framework. Biosens Bioelectron. 2016; 85:287-293. DOI: 10.1016/j.bios.2016.05.020. View

19.

Yilmaz M, Hsu S, Reinhoudt D, Velders A, Huskens J . Ratiometric fluorescent detection of an anthrax biomarker at molecular printboards. Angew Chem Int Ed Engl. 2010; 49(34):5938-41. DOI: 10.1002/anie.201000540. View