Molecular and Restriction Fragment Length Polymorphism Analysis of Canine Parvovirus 2 (CPV-2) in Dogs in Southeast Anatolia, Turkey

Overview

Journal Onderstepoort J Vet Res

Specialty Veterinary Medicine

Date 2019 Sep 4

PMID 31478735

Citations 7

Authors

Pelin F Polat

Adem Sahan

Gurbuz Aksoy

Mehmet O Timurkan

Ender Dincer

Affiliations

Soon will be listed here.

Abstract

Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting. Mutations of the CPV-2 genome have generated new variants circulating worldwide. This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey. Twenty blood samples previously taken for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR). Of the 20 samples, 18 tested positive for CPV-2. Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants. Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) variants showed sequences clustering separately in the phylogenetic tree. The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain. These results, which are the first to demonstrate the presence of CPV-2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey's dog population.

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References

Ikeda Y, Mochizuki M, Naito R, Nakamura K, Miyazawa T, Mikami T . Predominance of canine parvovirus (CPV) in unvaccinated cat populations and emergence of new antigenic types of CPVs in cats. Virology. 2000; 278(1):13-9. DOI: 10.1006/viro.2000.0653. View

Pratelli A, Cavalli A, Martella V, Tempesta M, Decaro N, Carmichael L . Canine parvovirus (CPV) vaccination: comparison of neutralizing antibody responses in pups after inoculation with CPV2 or CPV2b modified live virus vaccine. Clin Diagn Lab Immunol. 2001; 8(3):612-5. PMC: 96110. DOI: 10.1128/CDLI.8.3.612-615.2001. View

Battilani M, Scagliarini A, Tisato E, Turilli C, Jacoboni I, Casadio R . Analysis of canine parvovirus sequences from wolves and dogs isolated in Italy. J Gen Virol. 2001; 82(Pt 7):1555-1560. DOI: 10.1099/0022-1317-82-7-1555. View

Buonavoglia C, Martella V, Pratelli A, Tempesta M, Cavalli A, Buonavoglia D . Evidence for evolution of canine parvovirus type 2 in Italy. J Gen Virol. 2001; 82(Pt 12):3021-3025. DOI: 10.1099/0022-1317-82-12-3021. View

Nakamura M, Tohya Y, Miyazawa T, Mochizuki M, Phung H, Nguyen N . A novel antigenic variant of Canine parvovirus from a Vietnamese dog. Arch Virol. 2004; 149(11):2261-9. DOI: 10.1007/s00705-004-0367-y. View

Shackelton L, Parrish C, Truyen U, Holmes E . High rate of viral evolution associated with the emergence of carnivore parvovirus. Proc Natl Acad Sci U S A. 2005; 102(2):379-84. PMC: 544290. DOI: 10.1073/pnas.0406765102. View

Decaro N, Elia G, Desario C, Roperto S, Martella V, Campolo M . A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus. J Virol Methods. 2006; 136(1-2):65-70. PMC: 7119799. DOI: 10.1016/j.jviromet.2006.03.030. View

Battilani M, Scagliarini A, Ciulli S, Morganti L, Prosperi S . High genetic diversity of the VP2 gene of a canine parvovirus strain detected in a domestic cat. Virology. 2006; 352(1):22-6. DOI: 10.1016/j.virol.2006.06.002. View

Yesilbag K, Yilmaz Z, Ozkul A, Pratelli A . Aetiological role of viruses in puppies with diarrhoea. Vet Rec. 2007; 161(5):169-70. DOI: 10.1136/vr.161.5.169. View

10.

Gallo Calderon M, Mattion N, Bucafusco D, Fogel F, Remorini P, La Torre J . Molecular characterization of canine parvovirus strains in Argentina: Detection of the pathogenic variant CPV2c in vaccinated dogs. J Virol Methods. 2009; 159(2):141-5. DOI: 10.1016/j.jviromet.2009.03.013. View

11.

Yoon S, Jeong W, Kim H, An D . Molecular insights into the phylogeny of canine parvovirus 2 (CPV-2) with emphasis on Korean isolates: a Bayesian approach. Arch Virol. 2009; 154(8):1353-60. DOI: 10.1007/s00705-009-0444-3. View

12.

Demeter Z, Palade E, Soos T, Farsang A, Jakab C, Rusvai M . Misleading results of the MboII-based identification of type 2a canine parvovirus strains from Hungary reacting as type 2c strains. Virus Genes. 2010; 41(1):37-42. DOI: 10.1007/s11262-010-0478-3. View

13.

Castro T, Costa E, Leite J, Labarthe N, Cubel Garcia R . Monitoring of canine parvovirus (CPV) strains detected in vaccinated puppies in Brazil. Res Vet Sci. 2010; 90(2):336-40. DOI: 10.1016/j.rvsc.2010.06.005. View

14.

Ntafis V, Xylouri E, Kalli I, Desario C, Mari V, Decaro N . Characterization of Canine parvovirus 2 variants circulating in Greece. J Vet Diagn Invest. 2010; 22(5):737-40. DOI: 10.1177/104063871002200512. View

15.

Decaro N, Buonavoglia C . Canine parvovirus--a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Vet Microbiol. 2011; 155(1):1-12. PMC: 7173204. DOI: 10.1016/j.vetmic.2011.09.007. View

16.

Perez R, Bianchi P, Calleros L, Francia L, Hernandez M, Maya L . Recent spreading of a divergent canine parvovirus type 2a (CPV-2a) strain in a CPV-2c homogenous population. Vet Microbiol. 2011; 155(2-4):214-9. DOI: 10.1016/j.vetmic.2011.09.017. View

17.

Muz D, Oguzoglu T, Timurkan M, Akin H . Characterization of the partial VP2 gene region of canine parvoviruses in domestic cats from Turkey. Virus Genes. 2011; 44(2):301-8. DOI: 10.1007/s11262-011-0703-8. View

18.

Nandi S, Kumar M . Canine parvovirus: current perspective. Indian J Virol. 2013; 21(1):31-44. PMC: 3550768. DOI: 10.1007/s13337-010-0007-y. View

19.

Parthiban S, Mukhopadhyay H, Antony P, Pillai R . Molecular Typing of Canine parvovirus Occurring in Pondicherry by Multiplex PCR and PCR-RFLP. Indian J Virol. 2013; 21(1):86-9. PMC: 3550771. DOI: 10.1007/s13337-010-0011-2. View

20.

Sutton D, Vinberg C, Gustafsson A, Pearce J, Greenwood N . Canine parvovirus type 2c identified from an outbreak of severe gastroenteritis in a litter in Sweden. Acta Vet Scand. 2013; 55:64. PMC: 3846929. DOI: 10.1186/1751-0147-55-64. View