The Significance of Relative Claudin Expression in Odontogenic Tumors

Overview

Journal Head Neck Pathol

Specialties Oncology
Pathology

Date 2019 Sep 2

PMID 31473941

Citations 4

Authors

Ekarat Phattarataratip

Kraisorn Sappayatosok

Affiliations

Soon will be listed here.

Abstract

Claudins are integral to the structure and function of tight junctions. Altered claudin expression has been shown to affect disease behavior and patient prognosis in various neoplasms. The objectives of this study were to analyze the claudin-1, -4 and -7 expression in odontogenic tumors and characterize their expression pattern in distinct tumor cell types in relation to the recurrence potential. Sixty-nine cases of odontogenic tumors, including 43 ameloblastomas (AM), 17 adenomatoid odontogenic tumors (AOT), 6 ameloblastic fibromas (AF) and 3 ameloblastic carcinomas (AC) were investigated for claudin-1, -4 and -7 expression immunohistochemically. The staining was analyzed semi-quantitatively and categorized into 4 levels, based on the percentage of positively stained neoplastic epithelial cells. Claudin-1 was expressed in all AOT and AF cases, whereas most AC (66.7%) showed no expression. The claudin-1 staining was moderate-to-intense in the odontogenic epithelium of AF. In contrast, its staining of ameloblast-like cells and stellate reticulum-like cells in AM was weak. Claudin-7 expression was noted in all tumor types studied, while the expression of claudin-4 was limited and mainly localized in the squamous differentiated cells of AM and AC. AM showed significantly higher claudin-4, but lower claudin-7 expression than AOT. In addition, AC showed diminished claudin-1 immunoreactivity, compared to AOT. Low claudin-1 expression in AM was significantly associated with the increased clinical recurrence. The loss of claudin-1 may underlie the locally invasive nature of AM.

Citing Articles

Expression and clinical significance of CLDN7 and its immune-related cells in breast cancer.

Fan X, Qi A, Zhang M, Jia Y, Li S, Han D Diagn Pathol. 2024; 19(1):113.

PMID: 39175074 PMC: 11340154. DOI: 10.1186/s13000-024-01513-1.

Differential Expression of Claudin in Odontogenic Cysts.

Phattarataratip E, Sappayatosok K Eur J Dent. 2021; 16(2):320-326.

PMID: 34808689 PMC: 9339929. DOI: 10.1055/s-0041-1740440.

Identification of Claudin-6 as a Molecular Biomarker in Pan-Cancer Through Multiple Omics Integrative Analysis.

Zhang C, Guo C, Li Y, Liu K, Zhao Q, Ouyang L Front Cell Dev Biol. 2021; 9:726656.

PMID: 34409042 PMC: 8365468. DOI: 10.3389/fcell.2021.726656.

Expression of SOX2 and OCT4 in odontogenic cysts and tumors.

Phattarataratip E, Panitkul T, Khodkaew W, Anupuntanun P, Jaroonvechatam J, Pitarangsikul S Head Face Med. 2021; 17(1):29.

PMID: 34261507 PMC: 8278639. DOI: 10.1186/s13005-021-00283-1.

References

Severson E, Parkos C . Mechanisms of outside-in signaling at the tight junction by junctional adhesion molecule A. Ann N Y Acad Sci. 2009; 1165:10-8. DOI: 10.1111/j.1749-6632.2009.04034.x. View

Tsukita S, Tanaka H, Tamura A . The Claudins: From Tight Junctions to Biological Systems. Trends Biochem Sci. 2019; 44(2):141-152. DOI: 10.1016/j.tibs.2018.09.008. View

Pope J, Bhat A, Sharma A, Ahmad R, Krishnan M, Washington M . Claudin-1 regulates intestinal epithelial homeostasis through the modulation of Notch-signalling. Gut. 2013; 63(4):622-34. PMC: 4083824. DOI: 10.1136/gutjnl-2012-304241. View

Kwon M . Emerging roles of claudins in human cancer. Int J Mol Sci. 2013; 14(9):18148-80. PMC: 3794774. DOI: 10.3390/ijms140918148. View

Singh A, Dhawan P . Claudins and cancer: Fall of the soldiers entrusted to protect the gate and keep the barrier intact. Semin Cell Dev Biol. 2015; 42:58-65. DOI: 10.1016/j.semcdb.2015.05.001. View

Morohashi S, Kusumi T, Sato F, Odagiri H, Chiba H, Yoshihara S . Decreased expression of claudin-1 correlates with recurrence status in breast cancer. Int J Mol Med. 2007; 20(2):139-43. View

Sauer T, Pedersen M, Ebeltoft K, Naess O . Reduced expression of Claudin-7 in fine needle aspirates from breast carcinomas correlate with grading and metastatic disease. Cytopathology. 2005; 16(4):193-8. DOI: 10.1111/j.1365-2303.2005.00257.x. View

Usami Y, Chiba H, Nakayama F, Ueda J, Matsuda Y, Sawada N . Reduced expression of claudin-7 correlates with invasion and metastasis in squamous cell carcinoma of the esophagus. Hum Pathol. 2006; 37(5):569-77. DOI: 10.1016/j.humpath.2005.12.018. View

Sheehan G, Kallakury B, Sheehan C, Fisher H, Kaufman Jr R, Ross J . Loss of claudins-1 and -7 and expression of claudins-3 and -4 correlate with prognostic variables in prostatic adenocarcinomas. Hum Pathol. 2007; 38(4):564-9. DOI: 10.1016/j.humpath.2006.11.007. View

10.

Dhawan P, Singh A, Deane N, No Y, Shiou S, Schmidt C . Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer. J Clin Invest. 2005; 115(7):1765-76. PMC: 1150288. DOI: 10.1172/JCI24543. View

11.

Sappayatosok K, Phattarataratip E . Overexpression of Claudin-1 is Associated with Advanced Clinical Stage and Invasive Pathologic Characteristics of Oral Squamous Cell Carcinoma. Head Neck Pathol. 2014; 9(2):173-80. PMC: 4424206. DOI: 10.1007/s12105-014-0559-z. View

12.

Zhang W, Li W, Wang X, Hu Z, Zhu D, Ding W . CLDN1 expression in cervical cancer cells is related to tumor invasion and metastasis. Oncotarget. 2016; 7(52):87449-87461. PMC: 5350000. DOI: 10.18632/oncotarget.13871. View

13.

Konecny G, Agarwal R, Keeney G, Winterhoff B, Jones M, Mariani A . Claudin-3 and claudin-4 expression in serous papillary, clear-cell, and endometrioid endometrial cancer. Gynecol Oncol. 2008; 109(2):263-9. PMC: 2453046. DOI: 10.1016/j.ygyno.2008.01.024. View

14.

Resnick M, Gavilanez M, Newton E, Konkin T, Bhattacharya B, Britt D . Claudin expression in gastric adenocarcinomas: a tissue microarray study with prognostic correlation. Hum Pathol. 2005; 36(8):886-92. DOI: 10.1016/j.humpath.2005.05.019. View

15.

Bilodeau E, Prasad J, Alawi F, Seethala R . Molecular and genetic aspects of odontogenic lesions. Head Neck Pathol. 2014; 8(4):400-10. PMC: 4245404. DOI: 10.1007/s12105-014-0588-7. View

16.

Rizzitelli A, Smoll N, Chae M, Rozen W, Hunter-Smith D . Incidence and overall survival of malignant ameloblastoma. PLoS One. 2015; 10(2):e0117789. PMC: 4333213. DOI: 10.1371/journal.pone.0117789. View

17.

Chrcanovic B, Gomez R . Adenomatoid odontogenic tumor: An updated analysis of the cases reported in the literature. J Oral Pathol Med. 2018; 48(1):10-16. DOI: 10.1111/jop.12783. View

18.

Chrcanovic B, Brennan P, Rahimi S, Gomez R . Ameloblastic fibroma and ameloblastic fibrosarcoma: A systematic review. J Oral Pathol Med. 2017; 47(4):315-325. DOI: 10.1111/jop.12622. View

19.

Bello I, Soini Y, Slootweg P, Salo T . Claudins 1, 4, 5, 7 and occludin in ameloblastomas and developing human teeth. J Oral Pathol Med. 2006; 36(1):48-54. DOI: 10.1111/j.1600-0714.2006.00497.x. View

20.

Siar C, Abbas S . Claudin expression and tight junction protein localization in the lining epithelium of the keratocystic odontogenic tumors, dentigerous cysts, and radicular cysts. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013; 115(5):652-9. DOI: 10.1016/j.oooo.2013.02.013. View