Spectroscopic Characterization of Bovine, Avian and Johnin Purified Protein Derivative (PPD) with High-Throughput Fourier Transform InfraRed-Based Method

Overview

Journal Pathogens

Date 2019 Sep 1

PMID 31470609

Citations 4

Authors

Sara Corneli

Laura Corte

Luca Roscini

Antonella Di Paolo

Claudia Colabella

Linda Petrucci

Giulio Severi

Monica Cagiola

Piera Mazzone

Affiliations

Soon will be listed here.

Abstract

Tuberculins purified protein derivatives (PPDs) are obtained by precipitation from heat treated mycobacteria. PPDs are used in diagnosis of mycobacterial infections in humans and animals. Bovine PPD (PPDB) is obtained from ( complex), while Avian PPD (PPDA) and Johnin PPD (PPDJ) are extracted, respectively, from and ( complex). PPDB and PPDA are used for bovine tuberculosis diagnosis, while PPDJ is experimentally used in the immunodiagnosis of paratuberculosis. Although PPDs date back to the 19th Century, limited knowledge about their composition is currently available. The goal of our study was to evaluate Fourier Transform InfraRed (FTIR) spectroscopy as a tool to differentiate PPDB, PPDA, and three PPDJs. The results highlighted that the three PPDs have specific profiles, correlated with phylogenetic characteristics of mycobacteria used for their production. This analysis is eligible as a specific tool for different PPDs batches characterization and for the assessment of their composition. The entire PPD production may be efficiently controlled, since the N content of each preparation is related to IR spectra, with a reference spectrum for each PPD and a standardized analysis protocol.

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References

Wiker H . Unmatched sequences in public databases - exemplified by tuberculin-active protein. Scand J Immunol. 2004; 59(6):607-8. DOI: 10.1111/j.1365-3083.2004.1430.x. View

Zhao H, Kassama Y, Young M, Kell D, Goodacre R . Differentiation of Micromonospora isolates from a coastal sediment in Wales on the basis of Fourier transform infrared spectroscopy, 16S rRNA sequence analysis, and the amplified fragment length polymorphism technique. Appl Environ Microbiol. 2004; 70(11):6619-27. PMC: 525128. DOI: 10.1128/AEM.70.11.6619-6627.2004. View

Wenning M, Seiler H, Scherer S . Fourier-transform infrared microspectroscopy, a novel and rapid tool for identification of yeasts. Appl Environ Microbiol. 2002; 68(10):4717-21. PMC: 126420. DOI: 10.1128/AEM.68.10.4717-4721.2002. View

Rowland S, Ruckert J, Cummings P . Low molecular mass protein patterns in mycobacterial culture filtrates and purified protein derivatives. FEMS Immunol Med Microbiol. 1999; 23(1):21-5. DOI: 10.1111/j.1574-695X.1999.tb01712.x. View

Tang M, McEwen G, Wu Y, Miller C, Zhou A . Characterization and analysis of mycobacteria and Gram-negative bacteria and co-culture mixtures by Raman microspectroscopy, FTIR, and atomic force microscopy. Anal Bioanal Chem. 2012; 405(5):1577-91. DOI: 10.1007/s00216-012-6556-8. View

Auguste P, Tsertsvadze A, Pink J, Court R, McCarthy N, Sutcliffe P . Comparing interferon-gamma release assays with tuberculin skin test for identifying latent tuberculosis infection that progresses to active tuberculosis: systematic review and meta-analysis. BMC Infect Dis. 2017; 17(1):200. PMC: 5343308. DOI: 10.1186/s12879-017-2301-4. View

Rogall T, Wolters J, Flohr T, Bottger E . Towards a phylogeny and definition of species at the molecular level within the genus Mycobacterium. Int J Syst Bacteriol. 1990; 40(4):323-30. DOI: 10.1099/00207713-40-4-323. View

Eirin M, Macias A, Magnano G, Morsella C, Mendez L, Blanco F . Identification and evaluation of new Mycobacterium bovis antigens in the in vitro interferon gamma release assay for bovine tuberculosis diagnosis. Tuberculosis (Edinb). 2015; 95(6):795-801. DOI: 10.1016/j.tube.2015.07.009. View

Goodacre R, Timmins E, Rooney P, Rowland J, Kell D . Rapid identification of Streptococcus and Enterococcus species using diffuse reflectance-absorbance Fourier transform infrared spectroscopy and artificial neural networks. FEMS Microbiol Lett. 1996; 140(2-3):233-9. DOI: 10.1016/0378-1097(96)00186-3. View

10.

Yu C, Irudayaraj J . Spectroscopic characterization of microorganisms by Fourier transform infrared microspectroscopy. Biopolymers. 2005; 77(6):368-77. DOI: 10.1002/bip.20247. View

11.

Adt I, Toubas D, Pinon J, Manfait M, Sockalingum G . FTIR spectroscopy as a potential tool to analyse structural modifications during morphogenesis of Candida albicans. Arch Microbiol. 2006; 185(4):277-85. DOI: 10.1007/s00203-006-0094-8. View

12.

Szeghalmi A, Kaminskyj S, Gough K . A synchrotron FTIR microspectroscopy investigation of fungal hyphae grown under optimal and stressed conditions. Anal Bioanal Chem. 2006; 387(5):1779-89. DOI: 10.1007/s00216-006-0850-2. View

13.

Oberreuter H, Seiler H, Scherer S . Identification of coryneform bacteria and related taxa by Fourier-transform infrared (FT-IR) spectroscopy. Int J Syst Evol Microbiol. 2002; 52(Pt 1):91-100. DOI: 10.1099/00207713-52-1-91. View

14.

Lai S, Goodacre R, Manchester L . Whole-organism fingerprinting of the genus Carnobacterium using Fourier transform infrared spectroscopy (FT-IR). Syst Appl Microbiol. 2004; 27(2):186-91. DOI: 10.1078/072320204322881808. View

15.

Romha G, Gebru G, Asefa A, Mamo G . Epidemiology of Mycobacterium bovis and Mycobacterium tuberculosis in animals: Transmission dynamics and control challenges of zoonotic TB in Ethiopia. Prev Vet Med. 2018; 158:1-17. DOI: 10.1016/j.prevetmed.2018.06.012. View

16.

de la Rua-Domenech R . Human Mycobacterium bovis infection in the United Kingdom: Incidence, risks, control measures and review of the zoonotic aspects of bovine tuberculosis. Tuberculosis (Edinb). 2005; 86(2):77-109. DOI: 10.1016/j.tube.2005.05.002. View

17.

Borsuk S, Newcombe J, Mendum T, Dellagostin O, McFadden J . Identification of proteins from tuberculin purified protein derivative (PPD) by LC-MS/MS. Tuberculosis (Edinb). 2009; 89(6):423-30. DOI: 10.1016/j.tube.2009.07.003. View

18.

Yang H, Kruh-Garcia N, Dobos K . Purified protein derivatives of tuberculin--past, present, and future. FEMS Immunol Med Microbiol. 2012; 66(3):273-80. PMC: 3491170. DOI: 10.1111/j.1574-695X.2012.01002.x. View

19.

Ricchi M, Barbieri G, Taddei R, Belletti G, Carra E, Cammi G . Effectiveness of combination of Mini-and Microsatellite loci to sub-type Mycobacterium avium subsp. paratuberculosis Italian type C isolates. BMC Vet Res. 2011; 7:54. PMC: 3182896. DOI: 10.1186/1746-6148-7-54. View

20.

Wood P, Jones S . BOVIGAM: an in vitro cellular diagnostic test for bovine tuberculosis. Tuberculosis (Edinb). 2001; 81(1-2):147-55. DOI: 10.1054/tube.2000.0272. View