Protective Effects of Raspberry on the Oxidative Damage in HepG2 Cells Through Keap1/Nrf2-dependent Signaling Pathway

Overview

Journal Food Chem Toxicol

Specialties Nutritional Sciences
Toxicology

Date 2019 Aug 30

PMID 31465820

Citations 16

Authors

Lei Chen

Kang Li

Qian Liu

Jose L Quiles

Rosanna Filosa

Mohammad Amjad Kamal

Fang Wang

Guoyin Kai

Xiaobo Zou

Hui Teng

Jianbo Xiao

Affiliations

Soon will be listed here.

Abstract

The aim of the present study was to explore the protective effects of raspberry and its bioactive compound cyanidin 3-O-glucoside against HO-induced oxidative stress in HepG2 cells. We established a model of oxidative stress in HepG2 cells induced by HO and examined the protein expression of Keap1/Nrf2. The antioxidant activity of raspberry extract was carried out measuring the level of reactive oxygen species (ROS), and the changes of phase II detoxification elements such as GSH level and CAT activity. Also the expression of proteins related to the Keap1/Nrf2 signaling was tested. The results revealed that raspberry extract significantly reduced the ROS levels in oxidative injured cells, increased GSH content and CAT activity, and activated the expression of proteins Keap1, Nrf2, HO-1, NQO1, and γ-GCS. These results taken together indicated that raspberry treatment could ameliorate HO-induced oxidative stress in HepG2 cells via Keap1/Nrf2 pathway.

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