Unlimited Expansion of Intestinal Stem Cells from a Wide Range of Ages

Overview

Journal Integr Mol Med

Date 2019 Aug 30

PMID 31463081

Citations 1

Authors

Marcin Duleba

Rajasekaran Mahalingam

Audrey-Ann Liew

Yutao Qi

Rahul Neupane

Matthew Vincent

Suneal Agarwal

Francisco A Sylvester

Jeffrey S Hyams

Khek Yu Ho

Jason K Hou

Frank McKeon

Wa Xian

Affiliations

Soon will be listed here.

Abstract

The recent technical advance in cloning and culturing ground-state intestinal stem cells (ISC) provides us an opportunity of accurate assessment of age-related impact on the function of highly proliferative intestinal stem cells. Our ability of indefinitely and robustly expanding single-stem-cell derived pedigrees allows us to study intestinal stem cells at the clonal level. Interestingly, comparable number of ISC clones was yielded from 1mm endoscopic biopsy of all donors despite the age. They were passaged as pedigrees and expanded to 1 billion cells in approximately sixty days without changes in stemness demonstrated by clonogenicity and multipotency. Therefore, our study shows that ISCs from a wide range of ages can be cloned and expanded to unlimited number with similar efficiency and stability. These patient-derived ISCs harbor intrinsic immortality and are ideal for autologous transplantation, supporting the promise of adult-stem-cell based personalized medicine.

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