Antioxidant, Cytotoxicity, and Antiophidian Potential of Bark

Overview

Journal ACS Omega

Publisher American Chemical Society

Specialty Chemistry

Date 2019 Aug 29

PMID 31460040

Citations 3

Authors

Maria Carmen S Tan

Mary Stephanie S Carranza

Virgilio C Linis

Raymond S Malabed

Glenn G Oyong

Affiliations

Soon will be listed here.

Abstract

The objective of this research was to find the possible pharmacognosy of the bark of the Philippine Wall. ex G.Don (AM). Gas chromatographic-mass spectral (GC-EI-MS) characterization and energy dispersive X-ray spectroscopy (EDX) were performed to detect the bioactive constituents. EDX analysis of AM bark displayed a high content of potassium (3.26%) and calcium (2.96%). Eight constituents were detected in AM crude dichloromethane (DCM) extracts, which consisted of a long-chain unsaturated fatty acid (17:0) and fatty acid esters such as ethyl hexadecanoate and methyl hexadecanoate. Extraction of AM bark using methanol and dimethyl sulfoxide (MeOH/DMSO) solvents resulted in the identification of 17 constituents, principally alkaloids (alstonerine, 34.38%; strictamin, 5.23%; rauvomitin, 4.29%; and brucine, 3.66%) and triterpenoids (γ-sitosterol, 3.85%; lupeol, 3.00%; 24-methylenecycloartanol, 2.81%; campesterol, 2.71%; β-amyrin, 2.30%; and stigmasterol, 2.13%). MeOH/DMSO samples of AM were used in the selected bioassays. The samples exhibited efficient free radical scavenging activity (IC = 0.71 mg/mL) and were noncytotoxic to normal HDFn (IC > 100 μg/mL) and neoplastic THP-1 cell lines (IC = 67.22 μg/mL) while highly degenerative to MCF-7 (IC = 6.34 μg/mL), H69PR (IC = 7.05 μg/mL), and HT-29 (IC = 9.10 μg/mL). Most interestingly, the AM samples inhibited the northern Philippine Cobra's ( Taylor) venom (IC = 297.27 ± 9.33 μg/mL) through a secretory phospholipase A assay.

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