Numerical Operations in Living Cells by Programmable RNA Devices

Overview

Journal Sci Adv

Specialties Biology
Science

Date 2019 Aug 29

PMID 31457099

Citations 7

Authors

Kei Endo

Karin Hayashi

Hirohide Saito

Affiliations

Soon will be listed here.

Abstract

Integrated bioengineering systems can make executable decisions according to the cell state. To sense the state, multiple biomarkers are detected and processed via logic gates with synthetic biological devices. However, numerical operations have not been achieved. Here, we show a design principle for messenger RNA (mRNA) devices that recapitulates intracellular information by multivariate calculations in single living cells. On the basis of this principle and the collected profiles of multiple microRNA activities, we demonstrate that rationally programmed mRNA sets classify living human cells and track their change during differentiation. Our mRNA devices automatically perform multivariate calculation and function as a decision-maker in response to dynamic intracellular changes in living cells.

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