The Effects of Low-Dose Irradiation on Human Saliva: A Surface-Enhanced Raman Spectroscopy Study

Overview

Journal Diagnostics (Basel)

Specialty Radiology

Date 2019 Aug 25

PMID 31443529

Citations 10

Authors

Ioana Maria Colceriu-Simon

Mihaela Hedesiu

Valentin Toma

Gabriel Armencea

Alin Moldovan

Gabriela Stiufiuc

Bogdan Culic

Viorica Tarmure

Cristian Dinu

Ioana Berindan-Neagoe

Rares Ionut Stiufiuc

Mihaela Baciut

Affiliations

Soon will be listed here.

Abstract

Biological effects of low-dose ionizing radiation (IR) have been unclear until now. Saliva, because of the ease of collection, could be valuable in studying low-dose IR effects by means of surface-enhanced Raman spectroscopy (SERS). The objective of our study was to compare the salivary SER spectra recorded before and after low-dose IR exposure in the case of pediatric patients (PP). Unstimulated saliva was collected from ten PP before and after irradiation with a cone beam computed tomography (CBCT) machine used for diagnostic purposes. The SERS measurements have been recorded on dried saliva samples using a solid nanosilver plasmonic substrate synthesized using an original method developed in our laboratory. The experimental results showed that salivary SER spectra are dominated by three vibrational bands (441,735 and 2107 cm) that can be assigned to bending and stretching vibrations of salivary thiocyanate (SCN-). After exposure, an immediate increase of vibrational bands assigned to SCN- has been recorded in the case of all samples, probably as a result of IR interaction with oral cavity. This finding suggests that SCN- could be used as a valuable biomarker for the detection and identification of low-dose radiation effects.

Citing Articles

Understanding the Interaction of Röntgen Radiation Employed in Computed Tomography/Cone Beam Computed Tomography Investigations of the Oral Cavity by Means of Surface-Enhanced Raman Spectroscopy Analysis of Saliva.

Borsa R, Toma V, Nascutiu M, Onaciu A, Colceriu-Simon I, Baciut G Sensors (Basel). 2025; 24(24.

PMID: 39771757 PMC: 11679455. DOI: 10.3390/s24248021.

Label-Free SERS of Urine Components: A Powerful Tool for Discriminating Renal Cell Carcinoma through Multivariate Analysis and Machine Learning Techniques.

Buhas B, Toma V, Beauval J, Andras I, Couti R, Muntean L Int J Mol Sci. 2024; 25(7).

PMID: 38612705 PMC: 11011951. DOI: 10.3390/ijms25073891.

Saliva Collection Methods Among Children and Adolescents: A Scoping Review.

Fey J, Bikker F, Hesse D Mol Diagn Ther. 2023; 28(1):15-26.

PMID: 37950136 PMC: 10786738. DOI: 10.1007/s40291-023-00684-9.

High-Accuracy Renal Cell Carcinoma Discrimination through Label-Free SERS of Blood Serum and Multivariate Analysis.

Buhas B, Toma V, Crisan N, Ploussard G, Maghiar T, Stiufiuc R Biosensors (Basel). 2023; 13(8).

PMID: 37622899 PMC: 10452371. DOI: 10.3390/bios13080813.

Developing New Diagnostic Tools Based on SERS Analysis of Filtered Salivary Samples for Oral Cancer Detection.

Borsa R, Toma V, Onaciu A, Moldovan C, Marginean R, Cenariu D Int J Mol Sci. 2023; 24(15).

PMID: 37569501 PMC: 10418512. DOI: 10.3390/ijms241512125.

References

Michigami Y, Fujii K, Ueda K, Yamamoto Y . Determination of thiocyanate in human saliva and urine by ion chromatography. Analyst. 1992; 117(12):1855-8. DOI: 10.1039/an9921701855. View

Liu J, Duan Y . Saliva: a potential media for disease diagnostics and monitoring. Oral Oncol. 2012; 48(7):569-77. DOI: 10.1016/j.oraloncology.2012.01.021. View

Kalasinsky K, Hadfield T, Shea A, Kalasinsky V, Nelson M, Neiss J . Raman chemical imaging spectroscopy reagentless detection and identification of pathogens: signature development and evaluation. Anal Chem. 2007; 79(7):2658-73. DOI: 10.1021/ac0700575. View

Virkler K, Lednev I . Forensic body fluid identification: the Raman spectroscopic signature of saliva. Analyst. 2010; 135(3):512-7. DOI: 10.1039/b919393f. View

Stiufiuc R, Iacovita C, Stiufiuc G, Florea A, Achim M, Lucaciu C . A new class of pegylated plasmonic liposomes: synthesis and characterization. J Colloid Interface Sci. 2014; 437:17-23. DOI: 10.1016/j.jcis.2014.09.023. View

Qiu S, Xu Y, Huang L, Zheng W, Huang C, Huang S . Non-invasive detection of nasopharyngeal carcinoma using saliva surface-enhanced Raman spectroscopy. Oncol Lett. 2016; 11(1):884-890. PMC: 4727062. DOI: 10.3892/ol.2015.3969. View

Hujoel P, Hollender L, Bollen A, Young J, McGee M, Grosso A . Head-and-neck organ doses from an episode of orthodontic care. Am J Orthod Dentofacial Orthop. 2008; 133(2):210-7. DOI: 10.1016/j.ajodo.2007.10.026. View

Oenning A, Jacobs R, Pauwels R, Stratis A, Hedesiu M, Salmon B . Cone-beam CT in paediatric dentistry: DIMITRA project position statement. Pediatr Radiol. 2017; 48(3):308-316. DOI: 10.1007/s00247-017-4012-9. View

Bonifacio A, Dalla Marta S, Spizzo R, Cervo S, Steffan A, Colombatti A . Surface-enhanced Raman spectroscopy of blood plasma and serum using Ag and Au nanoparticles: a systematic study. Anal Bioanal Chem. 2014; 406(9-10):2355-65. DOI: 10.1007/s00216-014-7622-1. View

10.

Marcu M, Hedesiu M, Salmon B, Pauwels R, Stratis A, Oenning A . Estimation of the radiation dose for pediatric CBCT indications: a prospective study on ProMax3D. Int J Paediatr Dent. 2018; 28(3):300-309. DOI: 10.1111/ipd.12355. View

11.

Walkey C, Chan W . Understanding and controlling the interaction of nanomaterials with proteins in a physiological environment. Chem Soc Rev. 2011; 41(7):2780-99. DOI: 10.1039/c1cs15233e. View

12.

Bonifacio A, Cervo S, Sergo V . Label-free surface-enhanced Raman spectroscopy of biofluids: fundamental aspects and diagnostic applications. Anal Bioanal Chem. 2015; 407(27):8265-77. DOI: 10.1007/s00216-015-8697-z. View

13.

Stefancu A, Badarinza M, Moisoiu V, Iancu S, Serban O, Leopold N . SERS-based liquid biopsy of saliva and serum from patients with Sjögren's syndrome. Anal Bioanal Chem. 2019; 411(22):5877-5883. DOI: 10.1007/s00216-019-01969-x. View

14.

Bianchetta J, Bidaud J, Guidoni A, Bonicel J, ROVERY M . Porcine pancreatic lipase. Sequence of the first 234 amino acids of the peptide chain. Eur J Biochem. 1979; 97(2):395-405. DOI: 10.1111/j.1432-1033.1979.tb13126.x. View

15.

Pernot E, Cardis E, Badie C . Usefulness of saliva samples for biomarker studies in radiation research. Cancer Epidemiol Biomarkers Prev. 2014; 23(12):2673-80. DOI: 10.1158/1055-9965.EPI-14-0588. View

16.

Pernot E, Hall J, Baatout S, Benotmane M, Blanchardon E, Bouffler S . Ionizing radiation biomarkers for potential use in epidemiological studies. Mutat Res. 2012; 751(2):258-286. DOI: 10.1016/j.mrrev.2012.05.003. View

17.

Chandler J, Day B . Thiocyanate: a potentially useful therapeutic agent with host defense and antioxidant properties. Biochem Pharmacol. 2012; 84(11):1381-7. PMC: 3983948. DOI: 10.1016/j.bcp.2012.07.029. View

18.

Morgan P, Pattison D, Talib J, Summers F, Harmer J, Celermajer D . High plasma thiocyanate levels in smokers are a key determinant of thiol oxidation induced by myeloperoxidase. Free Radic Biol Med. 2011; 51(9):1815-22. DOI: 10.1016/j.freeradbiomed.2011.08.008. View

19.

Theodorakou C, Walker A, Horner K, Pauwels R, Bogaerts R, Jacobs R . Estimation of paediatric organ and effective doses from dental cone beam CT using anthropomorphic phantoms. Br J Radiol. 2012; 85(1010):153-60. PMC: 3473956. DOI: 10.1259/bjr/19389412. View

20.

Munro C, Grap M, Jablonski R, Boyle A . Oral health measurement in nursing research: state of the science. Biol Res Nurs. 2006; 8(1):35-42. PMC: 2213421. DOI: 10.1177/1099800406289343. View