Integrated Use of Strain CG163 and Acibenzolar-S-Methyl for Management of Bacterial Canker in Kiwifruit

Overview

Journal Plants (Basel)

Date 2019 Aug 25

PMID 31443158

Citations 13

Authors

Huub de Jong

Tony Reglinski

Philip A G Elmer

Kirstin Wurms

Joel L Vanneste

Lindy F Guo

Maryam Alavi

Affiliations

Soon will be listed here.

Abstract

An isolate of (strain = CG163) and the plant defence elicitor acibenzolar-S-methyl (ASM) were investigated for their ability to control leaf spot in kiwifruit caused by pv. biovar 3 (Psa). Clonal var. plantlets ('Hayward') were treated with ASM, CG163 or ASM + CG163 at seven and one day before inoculation with Psa. ASM (0.2 g/L) was applied either as a root or foliar treatments and CG163 was applied as a foliar spray containing 2 × 10 CFU/mL. Leaf spot incidence was significantly reduced by all treatments compared with the control. The combination of ASM + CG163 had greater efficacy (75%) than either ASM (55%) or CG163 (40%) alone. Moreover, treatment efficacy correlated positively with the expression of defence-related genes: pathogenesis-related protein 1 (PR1), β-1,3-glucosidase, Glucan endo 1,3-β-glucosidase (Gluc_PrimerH) and Class IV chitinase (ClassIV_Chit), with greater gene upregulation in plants treated with ASM + CG163 than by the individual treatments. Pathogen population studies indicated that CG163 had significant suppressive activity against epiphytic populations of Psa. Endophytic populations were reduced by ASM + CG163 but not by the individual treatments, and by 96-144 h after inoculation were significantly lower than the control. Together these data suggest that ASM + CG163 have complementary modes of action that contribute to greater control of leaf spotting than either treatment alone.

Citing Articles

Purification and expression of a novel bacteriocin, JUQZ-1, against pv. (PSA), secreted by Wq-1, isolated from the rhizosphere soil of healthy kiwifruit.

Shuai Y, Langbo Y, Yi Y, Danni C, Qingzhong P Front Microbiol. 2025; 15():1477320.

PMID: 39839114 PMC: 11747845. DOI: 10.3389/fmicb.2024.1477320.

Isolation and identification of a novel strain JIN4 and its potential for biocontrol of kiwifruit bacterial canker caused by pv. .

Zhao X, Zhai Y, Wei L, Xia F, Yang Y, Yi Y Front Plant Sci. 2025; 15:1513438.

PMID: 39764227 PMC: 11701161. DOI: 10.3389/fpls.2024.1513438.

Community structure of endophytic bacteria of Sargassum thubergii in the intertidal zone of Qingdao in China.

Li Y, Wang J, Sun T, Yu X, Yang Z, Zhao Y AMB Express. 2024; 14(1):35.

PMID: 38615116 PMC: 11016019. DOI: 10.1186/s13568-024-01688-2.

Scientific and technological advances in the development of sustainable disease management tools: a case study on kiwifruit bacterial canker.

Santos M, Nunes da Silva M, Vasconcelos M, Carvalho S Front Plant Sci. 2024; 14:1306420.

PMID: 38273947 PMC: 10808555. DOI: 10.3389/fpls.2023.1306420.

Kiwifruit Resistance to and pv. and Defence Induction by Acibenzolar-S-methyl and Methyl Jasmonate Are Cultivar Dependent.

Reglinski T, Wurms K, Vanneste J, Ah Chee A, Schipper M, Cornish D Int J Mol Sci. 2023; 24(21).

PMID: 37958935 PMC: 10647243. DOI: 10.3390/ijms242115952.

References

Xiong L, Lee H, Ishitani M, Zhu J . Regulation of osmotic stress-responsive gene expression by the LOS6/ABA1 locus in Arabidopsis. J Biol Chem. 2002; 277(10):8588-96. DOI: 10.1074/jbc.M109275200. View

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034. PMC: 126239. DOI: 10.1186/gb-2002-3-7-research0034. View

KING E, Ward M, RANEY D . Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 1954; 44(2):301-7. View

McGrath M, Andrews J . Temporal changes in microscale colonization of the phylloplane by Aureobasidium pullulans. Appl Environ Microbiol. 2006; 72(9):6234-41. PMC: 1563661. DOI: 10.1128/AEM.00744-06. View

McGrath M, Andrews J . Role of microbial immigration in the colonization of apple leaves by Aureobasidium pullulans. Appl Environ Microbiol. 2006; 73(4):1277-86. PMC: 1828642. DOI: 10.1128/AEM.01620-06. View

Morant A, Jorgensen K, Jorgensen C, Paquette S, Sanchez-Perez R, Moller B . beta-Glucosidases as detonators of plant chemical defense. Phytochemistry. 2008; 69(9):1795-813. DOI: 10.1016/j.phytochem.2008.03.006. View

Yan R, Hou J, Ding D, Guan W, Wang C, Wu Z . In vitro antifungal activity and mechanism of action of chitinase against four plant pathogenic fungi. J Basic Microbiol. 2008; 48(4):293-301. DOI: 10.1002/jobm.200700392. View

Herman M, Davidson J, Smart C . Induction of plant defense gene expression by plant activators and Pseudomonas syringae pv. tomato in greenhouse-grown tomatoes. Phytopathology. 2008; 98(11):1226-32. DOI: 10.1094/PHYTO-98-11-1226. View

Monteiro S, Barakat M, Picarra-Pereira M, Teixeira A, Ferreira R . Osmotin and thaumatin from grape: a putative general defense mechanism against pathogenic fungi. Phytopathology. 2008; 93(12):1505-12. DOI: 10.1094/PHYTO.2003.93.12.1505. View

10.

Vlot A, Dempsey D, Klessig D . Salicylic Acid, a multifaceted hormone to combat disease. Annu Rev Phytopathol. 2009; 47:177-206. DOI: 10.1146/annurev.phyto.050908.135202. View

11.

Tripathi D, Jiang Y, Kumar D . SABP2, a methyl salicylate esterase is required for the systemic acquired resistance induced by acibenzolar-S-methyl in plants. FEBS Lett. 2010; 584(15):3458-63. DOI: 10.1016/j.febslet.2010.06.046. View

12.

Cao F, Yoshioka K, Desveaux D . The roles of ABA in plant-pathogen interactions. J Plant Res. 2011; 124(4):489-99. DOI: 10.1007/s10265-011-0409-y. View

13.

Conrath U . Molecular aspects of defence priming. Trends Plant Sci. 2011; 16(10):524-31. DOI: 10.1016/j.tplants.2011.06.004. View

14.

Myresiotis C, Karaoglanidis G, Vryzas Z, Papadopoulou-Mourkidou E . Evaluation of plant-growth-promoting rhizobacteria, acibenzolar-S-methyl and hymexazol for integrated control of Fusarium crown and root rot on tomato. Pest Manag Sci. 2012; 68(3):404-11. DOI: 10.1002/ps.2277. View

15.

Mosqueira M, Willmann G, Zeiger U, Khurana T . Expression profiling reveals novel hypoxic biomarkers in peripheral blood of adult mice exposed to chronic hypoxia. PLoS One. 2012; 7(5):e37497. PMC: 3358260. DOI: 10.1371/journal.pone.0037497. View

16.

Chapman J, Taylor R, Weir B, Romberg M, Vanneste J, Luck J . Phylogenetic relationships among global populations of Pseudomonas syringae pv. actinidiae. Phytopathology. 2012; 102(11):1034-44. DOI: 10.1094/PHYTO-03-12-0064-R. View

17.

Reglinski T, Vanneste J, Wurms K, Gould E, Spinelli F, Rikkerink E . Using fundamental knowledge of induced resistance to develop control strategies for bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae. Front Plant Sci. 2013; 4:24. PMC: 3579167. DOI: 10.3389/fpls.2013.00024. View

18.

Meimoun P, Mordret E, Langlade N, Balzergue S, Arribat S, Bailly C . Is gene transcription involved in seed dry after-ripening?. PLoS One. 2014; 9(1):e86442. PMC: 3896479. DOI: 10.1371/journal.pone.0086442. View

19.

Acimovic S, Zeng Q, McGhee G, Sundin G, Wise J . Control of fire blight (Erwinia amylovora) on apple trees with trunk-injected plant resistance inducers and antibiotics and assessment of induction of pathogenesis-related protein genes. Front Plant Sci. 2015; 6:16. PMC: 4323746. DOI: 10.3389/fpls.2015.00016. View

20.

Hill M, Wurms K, Davy M, Gould E, Allan A, Mauchline N . Transcriptome Analysis of Kiwifruit (Actinidia chinensis) Bark in Response to Armoured Scale Insect (Hemiberlesia lataniae) Feeding. PLoS One. 2015; 10(11):e0141664. PMC: 4646472. DOI: 10.1371/journal.pone.0141664. View