Monitoring of Diffusion Properties and Transverse Relaxation Time of Mouse Ischaemic Muscle After Administration of Human Mesenchymal Stromal Cells Derived from Adipose Tissue

Overview

Journal Cell Prolif

Date 2019 Aug 24

PMID 31441162

Citations 2

Authors

Agnieszka Skorupa

Mateusz Ciszek

Ewelina Pilny

Ryszard Smolarczyk

Magdalena Jarosz-Biej

Lukasz Boguszewicz

Lukasz Krakowczyk

Stanislaw Szala

Maria Sokol

Tomasz Cichon

Affiliations

Soon will be listed here.

Abstract

Objectives: Application of non-invasive imaging methods plays an important role in the assessment of cellular therapy effects in peripheral artery disease. The purpose of this work was to evaluate the kinetics of MRI-derived parameters characterizing ischaemic hindlimb muscle after administration of human mesenchymal stromal cells derived from adipose tissue (hADSC) in mice.

Materials And Methods: MRI experiments were performed on a 9.4T Bruker system. The measurement protocol included transverse relaxation time mapping and diffusion tensor imaging. The monitoring period encompassed 14 days after femoral artery ligation and subsequent cell administration. The effect of hADSC transplantation was compared with the effect of normal human dermal fibroblasts (NHDFs) and phosphate-buffered saline injection.

Results: The most significant differences between the hADSC group and the remaining ones were observed around day 3 after ischaemia induction (increased transverse relaxation time in the hADSC group in comparison with the control group) and around day 7 (increased transverse relaxation time and decreased third eigenvalue of the diffusion tensor in the hADSC group in comparison with the control and NHDF groups) at the site of hADSC injection. Histologically, it was associated with increased macrophage infiltration at days 3-7 and with the presence of small regenerating fibres in the ischaemic tissue at day 7.

Conclusions: Our results underscore the important role of macrophages in mediating the therapeutic effects of hADSCs and confirm the huge potential of magnetic resonance imaging in monitoring of cellular therapy effects.

Citing Articles

In Vitro Cultures of Adipose-Derived Stem Cells: An Overview of Methods, Molecular Analyses, and Clinical Applications.

Jankowski M, Dompe C, Sibiak R, Wasiatycz G, Mozdziak P, Jaskowski J Cells. 2020; 9(8).

PMID: 32726947 PMC: 7463427. DOI: 10.3390/cells9081783.

Monitoring of diffusion properties and transverse relaxation time of mouse ischaemic muscle after administration of human mesenchymal stromal cells derived from adipose tissue.

Skorupa A, Ciszek M, Pilny E, Smolarczyk R, Jarosz-Biej M, Boguszewicz L Cell Prolif. 2019; 52(6):e12672.

PMID: 31441162 PMC: 6869084. DOI: 10.1111/cpr.12672.

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