A Ca/CaM-regulated Transcriptional Switch Modulates Stomatal Development in Response to Water Deficit

Overview

Journal Sci Rep

Specialty Science

Date 2019 Aug 24

PMID 31439865

Citations 12

Authors

Chan Yul Yoo

Noel Mano

Aliza Finkler

Hua Weng

Irene S Day

Anireddy S N Reddy

B W Poovaiah

Hillel Fromm

Paul M Hasegawa

Michael V Mickelbart

Affiliations

Soon will be listed here.

Abstract

Calcium (Ca) signals are decoded by the Ca-sensor protein calmodulin (CaM) and are transduced to Ca/CaM-binding transcription factors to directly regulate gene expression necessary for acclimation responses in plants. The molecular mechanisms of Ca/CaM signal transduction processes and their functional significance remains enigmatic. Here we report a novel Ca/CaM signal transduction mechanism that allosterically regulates DNA-binding activity of GT2-LIKE 1 (GTL1), a transrepressor of STOMATAL DENSITY AND DISTRIBUTION 1 (SDD1), to repress stomatal development in response to water stress. We demonstrated that Ca/CaM interaction with the 2 helix of the GTL1 N-terminal trihelix DNA-binding domain (GTL1N) destabilizes a hydrophobic core of GTL1N and allosterically inhibits 3 helix docking to the SDD1 promoter, leading to osmotic stress-induced Ca/CaM-dependent activation (de-repression) of SDD1 expression. This resulted in GTL1-dependent repression of stomatal development in response to water-deficit stress. Together, our results demonstrate that a Ca/CaM-regulated transcriptional switch on a trihelix transrepressor directly transduces osmotic stress to repress stomatal development to improve plant water-use efficiency as an acclimation response.

Citing Articles

Transcriptional repression of under water-deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance.

Mano N, Shaikh M, Widhalm J, Yoo C, Mickelbart M Plant Direct. 2024; 8(5):e594.

PMID: 38799417 PMC: 11117050. DOI: 10.1002/pld3.594.

Comprehensive Genomic Analysis of Trihelix Family in Tea Plant () and Their Putative Roles in Osmotic Stress.

Lang Z, Xu Z, Li L, He Y, Zhao Y, Zhang C Plants (Basel). 2024; 13(1).

PMID: 38202377 PMC: 10780335. DOI: 10.3390/plants13010070.

Genome-wide characterization and identification of Trihelix transcription factors and expression profiling in response to abiotic stresses in Chinese Willow ( Koidz).

Yang J, Tang Z, Yang W, Huang Q, Wang Y, Huang M Front Plant Sci. 2023; 14:1125519.

PMID: 36938039 PMC: 10020544. DOI: 10.3389/fpls.2023.1125519.

Different Leaf Anatomical Responses to Water Deficit in Maize and Soybean.

Mano N, Madore B, Mickelbart M Life (Basel). 2023; 13(2).

PMID: 36836647 PMC: 9966819. DOI: 10.3390/life13020290.

GT Transcription Factors of Thunb. Involved in Salt Stress Response.

Wang J, Cheng Y, Shi X, Feng L Biology (Basel). 2023; 12(2).

PMID: 36829455 PMC: 9952457. DOI: 10.3390/biology12020176.

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