Comparative Identification of MicroRNAs in Workers' Midguts in Responseto Invasion

Overview

Journal Insects

Specialty Biology

Date 2019 Aug 24

PMID 31438582

Citations 11

Authors

Dafu Chen

Yu Du

Huazhi Chen

Yuanchan Fan

Xiaoxue Fan

Zhiwei Zhu

Jie Wang

Cuiling Xiong

Yanzhen Zheng

Chunsheng Hou

Qingyun Diao

Rui Guo

Affiliations

Soon will be listed here.

Abstract

Here, the expression profiles and differentially expressed miRNAs (DEmiRNAs) in the midguts of workers at 7 d and 10 d post-inoculation (dpi) with were investigated via small RNA sequencing and bioinformatics. Five hundred and twenty nine (529) known miRNAs and 25 novel miRNAs were identified in this study, and the expression of 16 predicted miRNAs was confirmed by Stem-loop RT-PCR. A total of 14 DEmiRNAs were detected in the midgut at 7 dpi, including eight up-regulated and six down-regulated miRNAs, while 12 DEmiRNAs were observed in the midgut at 10 dpi, including nine up-regulated and three down-regulated ones. Additionally, five DEmiRNAs were shared, while nine and seven DEmiRNAs were specifically expressed in midguts at 7 dpi and 10 dpi. Gene ontology analysis suggested some DEmiRNAs and corresponding target mRNAs were involved in various functions including immune system processes and response to stimulus. KEGG pathway analysis shed light on the potential functions of some DEmiRNAs in regulating target mRNAs engaged in material and energy metabolisms, cellular immunity and the humoral immune system. Further investigation demonstrated a complex regulation network between DEmiRNAs and their target mRNAs, with miR-598-y, miR-252-y, miR-92-x and miR-3654-y at the center. Our results can facilitate future exploration of the regulatory roles of miRNAs in host responses to , and provide potential candidates for further investigation of the molecular mechanisms underlying eastern honeybee-microsporidian interactions.

Citing Articles

Transcriptomic Characterization of miRNAs in Larvae Responding to Infection.

Song Y, Qiu J, Kang J, Chen Y, Cao R, Wang W Genes (Basel). 2025; 16(2).

PMID: 40004485 PMC: 11855511. DOI: 10.3390/genes16020156.

Extensive influence of microsporidian infection on sucrose solution consumption, antioxidant enzyme activity, cell structure, and lifespan of Asian honeybees.

Fan X, Zhao H, Zang H, Dong S, Qiu J, Song Y Front Immunol. 2024; 15:1404766.

PMID: 39628478 PMC: 11611804. DOI: 10.3389/fimmu.2024.1404766.

Regulatory Roles of Long Non-Coding RNAs Relevant to Antioxidant Enzymes and Immune Responses of Larvae Following Invasion.

Guo R, Wang S, Guo S, Fan X, Zang H, Gao X Int J Mol Sci. 2023; 24(18).

PMID: 37762477 PMC: 10532054. DOI: 10.3390/ijms241814175.

Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts.

Fan X, Zhang W, Guo S, Zhu L, Zhang Y, Zhao H Insects. 2023; 14(5).

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Systematic Characterization and Regulatory Role of lncRNAs in Asian Honey Bees Responding to Microsporidian Infestation.

Wang Z, Wang S, Fan X, Zhang K, Zhang J, Zhao H Int J Mol Sci. 2023; 24(6).

PMID: 36982959 PMC: 10058195. DOI: 10.3390/ijms24065886.

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