Attenuates TNF-α-induced VCAM-1 and ICAM-1 Expression in Human Umbilical Vein Endothelial Cells

Overview

Journal J Taibah Univ Med Sci

Specialty General Medicine

Date 2019 Aug 23

PMID 31435328

Citations 6

Authors

Siti M Ismail

Uma M Sundar

Chua K Hui

Amilia Aminuddin

Azizah Ugusman

Affiliations

Soon will be listed here.

Abstract

Objectives: Inflammation plays a key role in the pathogenesis of atherosclerosis. is an herb with antioxidant and anti-atherosclerotic activities. The aim of this study was to evaluate the anti-inflammatory properties of an aqueous extract of (AEPS) in human umbilical vein endothelial cells (HUVECs).

Methods: HUVECs were divided into six groups: control, treatment with 10 ng/ml TNF-α, and co-treatment of 10 ng/ml TNF-α with four different concentrations of AEPS (100, 150, 250, and 300 μg/ml) for 24 h. Subsequently, vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) protein expression, U937 monocyte cells adhesion, and nuclear factor-kappaB (NF-κB) p65 expression in HUVECs were measured.

Results: Treatment of TNF-α-stimulated HUVECs with AEPS at different concentrations resulted in decreased VCAM-1 and ICAM-1 protein expression in a dose-dependent manner. Furthermore, AEPS also inhibited TNF-α-stimulated U937 monocyte cells adhesion to HUVECs. In addition, AEPS reduced TNF-α-induced NF-κB p65 expression in a dose-dependent manner.

Conclusions: The results indicated that AEPS suppressed TNF-α-induced VCAM-1 and ICAM-1 expression NF-κB signaling.

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