The Frequency of Chromosomal Euploidy Among 3PN Embryos

Overview

Journal J Reprod Infertil

Specialty Reproductive Medicine

Date 2019 Aug 20

PMID 31423415

Citations 10

Authors

Kresna Mutia

Budi Wiweko

Pritta Ameilia Iffanolida

Ririn Rahmala Febri

Naylah Muna

Oki Riayati

Shanty Olivia Jasirwan

Tita Yuningsih

Eliza Mansyur

Andon Hestiantoro

Affiliations

Soon will be listed here.

Abstract

Background: The evaluation of embryo morphology is one of the most important parameters used to evaluate developmental timing, also providing an indication of chromosomal failure or degeneration. The first step in the evaluation of a fertilization event is determining the number and shape of the pronuclei (PN). Normally fertilized eggs possess two even PN. However, some embryos which develop from abnormally fertilized zygotes may be tri-pronuclear zygotes (3PN).

Methods: Thirty embryos were collected from 12 women who underwent fertilization (IVF) at Dr. Cipto Mangunkusumo General Hospital in Jakarta, Indonesia. Embryos were cultured until the blastocyst stage on days 5-6. The blastomere biopsy was performed by piercing the zona pellucida with a laser under a microscope. Chromosomal numerical abnormalities were analyzed using Next Generation Sequencing (NGS).

Results: Among the 30 embryos with 3PN zygotes, 33.3% had a normal chromosomal array, with 22 pairs of autosomes and 2 pairs of sex chromosomes. While the rest of sample population detected as abnormal chromosome (66.7%), with the highest percentage of abnormality was triploidy 43.3%, followed by mosaicism 13.4% and aneuploidy 10%.

Conclusion: This was a preliminary study revealed not all morphologically 3PN embryos are genetically abnormal.

Citing Articles

The Identification of Molecular Ploidy Status of Abnormal Pronuclear Zygotes Reveals a Significant Number of Euploid Blastocysts Available for Conception.

McCallie B, Haywood M, Henry L, Lee R, Schoolcraft W, Katz-Jaffe M Biomedicines. 2025; 13(1).

PMID: 39857635 PMC: 11760476. DOI: 10.3390/biomedicines13010051.

The impact of low oocyte maturity ratio on blastocyst euploidy rate: a matched retrospective cohort study.

Ou Z, Du J, Liu N, Fang X, Wen X, Li J Contracept Reprod Med. 2024; 9(1):41.

PMID: 39187878 PMC: 11346022. DOI: 10.1186/s40834-024-00303-w.

Machine learning in time-lapse imaging to differentiate embryos from young vs old mice†.

Yang L, Leynes C, Pawelka A, Lorenzo I, Chou A, Lee B Biol Reprod. 2024; 110(6):1115-1124.

PMID: 38685607 PMC: 11180621. DOI: 10.1093/biolre/ioae056.

Evaluating the developmental potential of 2.1PN-derived embryos and associated chromosomal analysis.

Wang J, Xiong S, Hao X, Gao Y, Xia F, Liao H J Assist Reprod Genet. 2024; 41(6):1597-1603.

PMID: 38613651 PMC: 11224204. DOI: 10.1007/s10815-024-03113-w.

The utility of human two plus one small pronucleated zygotes (2.1PN) based on clinical outcomes and the focused ploidy analysis.

Hattori H, Okuyama N, Ashikawa K, Sakuraba Y, Igarashi H, Kyono K J Assist Reprod Genet. 2024; 41(6):1589-1596.

PMID: 38613650 PMC: 11224203. DOI: 10.1007/s10815-024-03114-9.

References

Munne S, Cohen J . Chromosome abnormalities in human embryos. Hum Reprod Update. 1999; 4(6):842-55. DOI: 10.1093/humupd/4.6.842. View

SACHS A, Politch J, Jackson K, Racowsky C, Hornstein M, Ginsburg E . Factors associated with the formation of triploid zygotes after intracytoplasmic sperm injection. Fertil Steril. 2000; 73(6):1109-14. DOI: 10.1016/s0015-0282(00)00521-5. View

Magli M, Jones G, Gras L, Gianaroli L, Korman I, Trounson A . Chromosome mosaicism in day 3 aneuploid embryos that develop to morphologically normal blastocysts in vitro. Hum Reprod. 2000; 15(8):1781-6. DOI: 10.1093/humrep/15.8.1781. View

Feng H, Hershlag A . Fertilization abnormalities following human in vitro fertilization and intracytoplasmic sperm injection. Microsc Res Tech. 2003; 61(4):358-61. DOI: 10.1002/jemt.10349. View

Porter R, Han T, Tucker M, Graham J, Liebermann J, Scott Sills E . Estimation of second polar body retention rate after conventional insemination and intracytoplasmic sperm injection: in vitro observations from more than 5000 human oocytes. J Assist Reprod Genet. 2003; 20(9):371-6. PMC: 3455839. DOI: 10.1023/a:1025481011680. View

Rienzi L, Ubaldi F, Iacobelli M, Romano S, Minasi M, Ferrero S . Significance of morphological attributes of the early embryo. Reprod Biomed Online. 2005; 10(5):669-81. DOI: 10.1016/s1472-6483(10)61676-8. View

Chen Z, Yan J, Feng H . Aneuploid analysis of tripronuclear zygotes derived from in vitro fertilization and intracytoplasmic sperm injection in humans. Fertil Steril. 2005; 83(6):1845-8. DOI: 10.1016/j.fertnstert.2004.11.076. View

Rosen M, Shen S, Dobson A, Fujimoto V, McCulloch C, Cedars M . Triploidy formation after intracytoplasmic sperm injection may be a surrogate marker for implantation. Fertil Steril. 2006; 85(2):384-90. DOI: 10.1016/j.fertnstert.2005.07.1321. View

Feenan K, Herbert M . Can 'abnormally' fertilized zygotes give rise to viable embryos?. Hum Fertil (Camb). 2006; 9(3):157-69. DOI: 10.1080/14647270600636269. View

10.

Dale B, DeFelice L . Polyspermy prevention: facts and artifacts?. J Assist Reprod Genet. 2010; 28(3):199-207. PMC: 3082659. DOI: 10.1007/s10815-010-9513-5. View

11.

Grau N, Escrich L, Martin J, Rubio C, Pellicer A, Escriba M . Self-correction in tripronucleated human embryos. Fertil Steril. 2011; 96(4):951-6. DOI: 10.1016/j.fertnstert.2011.07.1087. View

12.

Montag M, Toth B, Strowitzki T . New approaches to embryo selection. Reprod Biomed Online. 2013; 27(5):539-46. DOI: 10.1016/j.rbmo.2013.05.013. View

13.

Bianchi E, Wright G . Izumo meets Juno: preventing polyspermy in fertilization. Cell Cycle. 2014; 13(13):2019-20. PMC: 4111690. DOI: 10.4161/cc.29461. View

14.

Itoi F, Asano Y, Shimizu M, Honnma H, Murata Y . Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes. J Assist Reprod Genet. 2015; 32(9):1401-7. PMC: 4595390. DOI: 10.1007/s10815-015-0518-y. View

15.

Li M, Zhao W, Xue X, Zhang S, Shi W, Shi J . Three pro-nuclei (3PN) incidence factors and clinical outcomes: a retrospective study from the fresh embryo transfer of in vitro fertilization with donor sperm (IVF-D). Int J Clin Exp Med. 2015; 8(8):13997-4003. PMC: 4613043. View

16.

Sallam H, Sallam N, Sallam S . Non-invasive methods for embryo selection. Facts Views Vis Obgyn. 2016; 8(2):87-100. PMC: 5130307. View

17.

Bradley C, Traversa M, Hobson N, Gee A, McArthur S . Clinical use of monopronucleated zygotes following blastocyst culture and preimplantation genetic screening, including verification of biparental chromosome inheritance. Reprod Biomed Online. 2017; 34(6):567-574. DOI: 10.1016/j.rbmo.2017.03.013. View

18.

Mateo S, Vidal F, Parriego M, Rodriguez I, Montalvo V, Veiga A . Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS. J Assist Reprod Genet. 2017; 34(7):905-911. PMC: 5476548. DOI: 10.1007/s10815-017-0937-z. View

19.

Yalcinkaya E, Ozay A, Ergin E, Oztel Z, Ozornek H . Live birth after transfer of a tripronuclear embryo: An intracytoplasmic sperm injection as a combination of microarray and time-lapse technology. Turk J Obstet Gynecol. 2017; 13(2):95-98. PMC: 5558346. DOI: 10.4274/tjod.45144. View

20.

Kola I, Trounson A, Dawson G, Rogers P . Tripronuclear human oocytes: altered cleavage patterns and subsequent karyotypic analysis of embryos. Biol Reprod. 1987; 37(2):395-401. DOI: 10.1095/biolreprod37.2.395. View