Goniothalamin Induces Necroptosis and Anoikis in Human Invasive Breast Cancer MDA-MB-231 Cells

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Aug 17

PMID 31416203

Citations 8

Authors

Patompong Khaw-On

Wilart Pompimon

Ratana Banjerdpongchai

Affiliations

Soon will be listed here.

Abstract

Goniothalamin (GTN) is toxic to several types of cancer cells in vitro. However, its effects on non-apoptotic cell death induction of human cancer cells have been poorly documented. Here, an investigation of the anti-cancer activity of GTN and the molecular signaling pathways of non-apoptotic cell death in the invasive human breast cancer MDA-MB-231 cell line were undertaken. Apoptotic cell death was suppressed by using a pan-caspase inhibitor (Benzyloxycarbonyl-Val-Ala-Asp-[O-methyl]-fluoromethylketone), z-VAD-fmk) as a model to study whether GTN induced caspase-independent cell death. In the anoikis study, MDA-MB-231 cells were cultured on poly-(2-hydroxyethyl methacrylate)- or poly-HEMA- coated plates to mimic anoikis-resistance growth and determine whether GTN induced cell death and the mechanisms involved. GTN and z-VAD-fmk induced human breast cancer MDA-MB-231 cells to undergo necroptosis via endoplasmic reticulum (ER) and oxidative stresses, with increased expressions of necroptotic genes such as , , and . GTN induced MDA-MB-231 cells to undergo anoikis via reversed epithelial-mesenchymal transition (EMT) protein expressions, inhibited the EGFR/FAK/Src survival signaling pathway, and decreased matrix metalloproteinase secretion.

Citing Articles

Metabolic reprogramming and signaling adaptations in anoikis resistance: mechanisms and therapeutic targets.

He C, He J Mol Cell Biochem. 2025; .

PMID: 39821582 DOI: 10.1007/s11010-024-05199-3.

Dual roles of inflammatory programmed cell death in cancer: insights into pyroptosis and necroptosis.

Wang S, He H, Qu L, Shen Q, Dai Y Front Pharmacol. 2024; 15:1446486.

PMID: 39257400 PMC: 11384570. DOI: 10.3389/fphar.2024.1446486.

An Organofluorine Isoselenocyanate Analogue of Sulforaphane Affects Antimetabolite 5-Fluorouracil's Anticancer Activity: A Perspective for New Combinatory Therapy in Triple-Negative Breast Cancer.

Milczarek M, Cierpial T, Kielbasinski P, Malecka-Gieldowska M, Switalska M, Wietrzyk J Molecules. 2023; 28(15).

PMID: 37570783 PMC: 10420864. DOI: 10.3390/molecules28155808.

Anoikis resistance--protagonists of breast cancer cells survive and metastasize after ECM detachment.

Dai Y, Zhang X, Ou Y, Zou L, Zhang D, Yang Q Cell Commun Signal. 2023; 21(1):190.

PMID: 37537585 PMC: 10399053. DOI: 10.1186/s12964-023-01183-4.

Necroptosis Induced by Delta-Tocotrienol Overcomes Docetaxel Chemoresistance in Prostate Cancer Cells.

Montagnani Marelli M, Beretta G, Moretti R Int J Mol Sci. 2023; 24(5).

PMID: 36902362 PMC: 10003232. DOI: 10.3390/ijms24054923.

References

Inayat-Hussain S, Osman A, Din L, Ali A, Snowden R, MacFarlane M . Caspases-3 and -7 are activated in goniothalamin-induced apoptosis in human Jurkat T-cells. FEBS Lett. 1999; 456(3):379-83. DOI: 10.1016/s0014-5793(99)00984-9. View

Inayat-Hussain S, Annuar B, Din L, Ali A, Ross D . Loss of mitochondrial transmembrane potential and caspase-9 activation during apoptosis induced by the novel styryl-lactone goniothalamin in HL-60 leukemia cells. Toxicol In Vitro. 2003; 17(4):433-9. DOI: 10.1016/s0887-2333(03)00051-1. View

Cande C, Vahsen N, Garrido C, Kroemer G . Apoptosis-inducing factor (AIF): caspase-independent after all. Cell Death Differ. 2004; 11(6):591-5. DOI: 10.1038/sj.cdd.4401400. View

Broker L, Kruyt F, Giaccone G . Cell death independent of caspases: a review. Clin Cancer Res. 2005; 11(9):3155-62. DOI: 10.1158/1078-0432.CCR-04-2223. View

Chen W, Wu C, Lan Y, Chang F, Teng C, Wu Y . Goniothalamin induces cell cycle-specific apoptosis by modulating the redox status in MDA-MB-231 cells. Eur J Pharmacol. 2005; 522(1-3):20-9. DOI: 10.1016/j.ejphar.2005.08.047. View

Gilmore A . Anoikis. Cell Death Differ. 2005; 12 Suppl 2:1473-7. DOI: 10.1038/sj.cdd.4401723. View

Zhang L, Hao Y, Hu C, Ye Y, Xie Q, Thorne R . Inhibition of apoptosis facilitates necrosis induced by cisplatin in gastric cancer cells. Anticancer Drugs. 2008; 19(2):159-66. DOI: 10.1097/CAD.0b013e3282f30d05. View

Han W, Xie J, Li L, Liu Z, Hu X . Necrostatin-1 reverts shikonin-induced necroptosis to apoptosis. Apoptosis. 2009; 14(5):674-86. DOI: 10.1007/s10495-009-0334-x. View

Demers M, Thibodeau S, Noel D, Fujita N, Tsuruo T, Gauthier R . Intestinal epithelial cancer cell anoikis resistance: EGFR-mediated sustained activation of Src overrides Fak-dependent signaling to MEK/Erk and/or PI3-K/Akt-1. J Cell Biochem. 2009; 107(4):639-54. DOI: 10.1002/jcb.22131. View

10.

Cho Y, Challa S, Moquin D, Genga R, Ray T, Guildford M . Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation. Cell. 2009; 137(6):1112-23. PMC: 2727676. DOI: 10.1016/j.cell.2009.05.037. View

11.

Inayat-Hussain S, Chan K, Rajab N, Din L, Chow S, Kizilors A . Goniothalamin-induced oxidative stress, DNA damage and apoptosis via caspase-2 independent and Bcl-2 independent pathways in Jurkat T-cells. Toxicol Lett. 2009; 193(1):108-14. PMC: 2828539. DOI: 10.1016/j.toxlet.2009.12.010. View

12.

Chiu C, Liu P, Huang K, Wang H, Chang K, Chou C . Goniothalamin inhibits growth of human lung cancer cells through DNA damage, apoptosis, and reduced migration ability. J Agric Food Chem. 2011; 59(8):4288-93. DOI: 10.1021/jf200566a. View

13.

Al-Qubaisi M, Rozita R, Yeap S, Omar A, Ali A, Alitheen N . Selective cytotoxicity of goniothalamin against hepatoblastoma HepG2 cells. Molecules. 2011; 16(4):2944-59. PMC: 6260619. DOI: 10.3390/molecules16042944. View

14.

Wood L, Shillitoe E . Effect of a caspase inhibitor, zVADfmk, on the inhibition of breast cancer cells by herpes simplex virus type 1. Cancer Gene Ther. 2011; 18(10):685-94. DOI: 10.1038/cgt.2011.34. View

15.

Cabon L, Galan-Malo P, Bouharrour A, Delavallee L, Brunelle-Navas M, Lorenzo H . BID regulates AIF-mediated caspase-independent necroptosis by promoting BAX activation. Cell Death Differ. 2011; 19(2):245-56. PMC: 3263500. DOI: 10.1038/cdd.2011.91. View

16.

Sun L, Yau H, Lau O, Huang Y, Yao X . Effect of hydrogen peroxide and superoxide anions on cytosolic Ca2+: comparison of endothelial cells from large-sized and small-sized arteries. PLoS One. 2011; 6(9):e25432. PMC: 3180448. DOI: 10.1371/journal.pone.0025432. View

17.

Vanlangenakker N, Bertrand M, Bogaert P, Vandenabeele P, Vanden Berghe T . TNF-induced necroptosis in L929 cells is tightly regulated by multiple TNFR1 complex I and II members. Cell Death Dis. 2011; 2:e230. PMC: 3223695. DOI: 10.1038/cddis.2011.111. View

18.

Kolacinska A, Chalubinska J, Zawlik I, Szymanska B, Borowska-Garganisz E, Nowik M . Apoptosis-, proliferation, immune function-, and drug resistance- related genes in ER positive, HER2 positive and triple negative breast cancer. Neoplasma. 2012; 59(4):424-32. DOI: 10.4149/neo_2012_055. View

19.

Kim Y, Koo K, Sung J, Yun U, Kim H . Anoikis resistance: an essential prerequisite for tumor metastasis. Int J Cell Biol. 2012; 2012:306879. PMC: 3296207. DOI: 10.1155/2012/306879. View

20.

Yen C, Chiu C, Haung R, Yeh C, Huang K, Chang K . Antiproliferative effects of goniothalamin on Ca9-22 oral cancer cells through apoptosis, DNA damage and ROS induction. Mutat Res. 2012; 747(2):253-8. DOI: 10.1016/j.mrgentox.2012.06.003. View