CYRI/ Fam49 Proteins Represent a New Class of Rac1 Interactors

Overview

Journal Commun Integr Biol

Specialty Biology

Date 2019 Aug 16

PMID 31413787

Citations 7

Authors

Jamie A Whitelaw

Sergio Lilla

Nikki R Paul

Loic Fort

Sara Zanivan

Laura M Machesky

Affiliations

Soon will be listed here.

Abstract

Fam49 proteins, now referred to as CYRI (CYFIP-related Rac Interactor), are evolutionarily conserved across many phyla. Their closest relative by amino acid sequence is CYFIP, as both proteins contain a domain of unknown function DUF1394. We recently showed that CYRI and the DUF1394 can mediate binding to Rac1 and evidence is building to suggest that CYRI plays important roles in cell migration, chemotaxis and pathogen entry into cells. Here we discuss how CYRI proteins fit into the current framework of the control of actin dynamics by positive and negative feedback loops containing Rac1, the Scar/WAVE Complex, the Arp2/3 Complex and branched actin. We also provide data regarding the interaction between Rac1 and CYRI in an unbiassed mass spectrometry screen for interactors of an active mutant of Rac1.

Citing Articles

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Differential Role of the RAC1-Binding Proteins FAM49b (CYRI-B) and CYFIP1 in Platelets.

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Structural Basis of CYRI-B Direct Competition with Scar/WAVE Complex for Rac1.

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