Expansion of Typhi Clonal Lineages with Ampicillin Resistance and Reduced Ciprofloxacin Susceptibility in Eastern China

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2019 Aug 16

PMID 31413599

Citations 2

Authors

Dingfeng Lv

Danyang Zhang

Qifa Song

Affiliations

Soon will be listed here.

Abstract

Purpose: This study was aimed to investigate the dynamics of antimicrobial resistance expansion among different lineages and isolates of . Typhi.

Materials And Methods: The . Typhi isolates were collected from the patients clinically suspected of typhoid fever in Eastern China during 2005-2017. All isolates were tested retrospectively for susceptibility to eight antimicrobials and the genes related to quinolone and ampicillin resistance, including and . The isolates were subtyped by PFGE.

Results: Of 140 isolates, all were susceptible to ciprofloxacin, cefotaxime, chloramphenicol, and trimethoprim-sulfamethoxazole, 95 (68%) were nalidixic acid resistant, and 74 (53%) were ampicillin resistant. The resistance to ampicillin and nalidixic acid was first observed in 2006. Among the 95 nalidixic acid-resistant . Typhi isolates, 62 possessed S83F mutation in and 25 possessed D87Y mutation. All ampicillin-resistant isolates harbored gene . PFGE generated 47 distinguishable clonal lineages. Overall, 64% (89/140) belonged to seven prevalent lineages of clustering isolates. PFGE results illustrated the prevalence of nalidixic acid-resistant lineages increased steadily from 19% during 2005-2012 to 50% during 2013-2014, and thereafter to 74% during 2015-2017 and similar development of ampicillin-resistant lineages increased from 6% to 38%, and also to 39%.

Conclusion: The present study indicated the clonal expansion of . Typhi with ampicillin resistance and reduced ciprofloxacin susceptibility. The findings also suggested that the differential development of antimicrobial resistance to various antimicrobial agents in . Typhi, showing the rapid increase in ampicillin resistance and reduced ciprofloxacin susceptibility, and the high susceptibility to other traditional antimicrobial agents.

Citing Articles

Prevalence of Antimicrobial Resistance Genes in Typhi: A Systematic Review and Meta-Analysis.

Yusof N, Norazzman N, Mohd Zaidi N, Mohd Azlan M, Ghazali B, Ahmad Najib M Trop Med Infect Dis. 2022; 7(10).

PMID: 36288012 PMC: 9611315. DOI: 10.3390/tropicalmed7100271.

Prevalence of and Antimicrobial Resistance in Isolates from Food Animals - Six PLADs, China, 2019.

Cao T, Liu P, Li Y, Cui M, Zhang C, Wang Y China CDC Wkly. 2021; 3(24):514-517.

PMID: 34594924 PMC: 8393044. DOI: 10.46234/ccdcw2021.132.

References

Cooke F, Wain J, Threlfall E . Fluoroquinolone resistance in Salmonella Typhi. BMJ. 2006; 333(7563):353-4. PMC: 1539082. DOI: 10.1136/bmj.333.7563.353-b. View

Kato Y, Fukayama M, Adachi T, Imamura A, Tsunoda T, Takayama N . Multidrug-resistant typhoid fever outbreak in travelers returning from Bangladesh. Emerg Infect Dis. 2008; 13(12):1954-5. PMC: 2876753. DOI: 10.3201/eid1312.070422. View

Vien L, Baker S, Phuong Thao L, Tu L, Thuy C, Nga T . High prevalence of plasmid-mediated quinolone resistance determinants in commensal members of the Enterobacteriaceae in Ho Chi Minh City, Vietnam. J Med Microbiol. 2009; 58(Pt 12):1585-1592. PMC: 2884939. DOI: 10.1099/jmm.0.010033-0. View

Kumar Y, Sharma A, Mani K . Re-emergence of susceptibility to conventionally used drugs among strains of Salmonella Typhi in central west India. J Infect Dev Ctries. 2011; 5(3):227-30. DOI: 10.3855/jidc.1310. View

Effa E, Lassi Z, Critchley J, Garner P, Sinclair D, Olliaro P . Fluoroquinolones for treating typhoid and paratyphoid fever (enteric fever). Cochrane Database Syst Rev. 2011; (10):CD004530. PMC: 6532575. DOI: 10.1002/14651858.CD004530.pub4. View

Ahmed D, Hoque A, Mazumder R, Nahar K, Islam N, Gazi S . Salmonella enterica serovar Typhi strain producing extended-spectrum β-lactamases in Dhaka, Bangladesh. J Med Microbiol. 2012; 61(Pt 7):1032-1033. DOI: 10.1099/jmm.0.044065-0. View

Zarrilli R, Pournaras S, Giannouli M, Tsakris A . Global evolution of multidrug-resistant Acinetobacter baumannii clonal lineages. Int J Antimicrob Agents. 2012; 41(1):11-9. DOI: 10.1016/j.ijantimicag.2012.09.008. View

Garg N, Tewari R, Gupta V, Kapil A, Kumar S, Rishi P . Current antibiogram and clonal relatedness among drug-resistant Salmonella enterica serovar Typhi in Northern India. Microb Drug Resist. 2013; 19(3):204-11. DOI: 10.1089/mdr.2012.0071. View

Hassing R, Goessens W, Mevius D, Van Pelt W, Mouton J, Verbon A . Decreased ciprofloxacin susceptibility in Salmonella Typhi and Paratyphi infections in ill-returned travellers: the impact on clinical outcome and future treatment options. Eur J Clin Microbiol Infect Dis. 2013; 32(10):1295-301. DOI: 10.1007/s10096-013-1878-9. View

10.

Huddleston J . Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes. Infect Drug Resist. 2014; 7:167-76. PMC: 4073975. DOI: 10.2147/IDR.S48820. View

11.

Rahman B, Wasfy M, Maksoud M, Hanna N, Dueger E, House B . Multi-drug resistance and reduced susceptibility to ciprofloxacin among Salmonella enterica serovar Typhi isolates from the Middle East and Central Asia. New Microbes New Infect. 2014; 2(4):88-92. PMC: 4184576. DOI: 10.1002/nmi2.46. View

12.

Wong V, Baker S, Pickard D, Parkhill J, Page A, Feasey N . Phylogeographical analysis of the dominant multidrug-resistant H58 clade of Salmonella Typhi identifies inter- and intracontinental transmission events. Nat Genet. 2015; 47(6):632-9. PMC: 4921243. DOI: 10.1038/ng.3281. View

13.

Yan M, Li X, Liao Q, Li F, Zhang J, Kan B . The emergence and outbreak of multidrug-resistant typhoid fever in China. Emerg Microbes Infect. 2016; 5:e62. PMC: 4932652. DOI: 10.1038/emi.2016.62. View

14.

. Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980-2015: a systematic analysis for the Global Burden of Disease Study 2015. Lancet. 2016; 388(10053):1459-1544. PMC: 5388903. DOI: 10.1016/S0140-6736(16)31012-1. View

15.

Song Q, Yang Y, Lin W, Yi B, Xu G . Epidemiological Characteristics and Clinical Treatment Outcome of Typhoid Fever in Ningbo, China, 2005-2014: Pulsed-Field Gel Electorophoresis Results Revealing Great Proportion of Common Transmission Sources. Jpn J Infect Dis. 2017; 70(5):513-517. DOI: 10.7883/yoken.JJID.2016.434. View

16.

Song Q, Xu Z, Gao H, Zhang D . Overview of the development of quinolone resistance in species in China, 2005-2016. Infect Drug Resist. 2018; 11:267-274. PMC: 5833789. DOI: 10.2147/IDR.S157460. View