Apical Polarity Proteins Recruit the RhoGEF Cysts to Promote Junctional Myosin Assembly

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2019 Aug 15

PMID 31409654

Citations 16

Authors

Jordan T Silver

Frederik Wirtz-Peitz

Sergio Simoes

Milena Pellikka

Dong Yan

Richard Binari

Takashi Nishimura

Yan Li

Tony J C Harris

Norbert Perrimon

Ulrich Tepass

Affiliations

Soon will be listed here.

Abstract

The spatio-temporal regulation of small Rho GTPases is crucial for the dynamic stability of epithelial tissues. However, how RhoGTPase activity is controlled during development remains largely unknown. To explore the regulation of Rho GTPases in vivo, we analyzed the Rho GTPase guanine nucleotide exchange factor (RhoGEF) Cysts, the orthologue of mammalian p114RhoGEF, GEF-H1, p190RhoGEF, and AKAP-13. Loss of Cysts causes a phenotype that closely resembles the mutant phenotype of the apical polarity regulator Crumbs. This phenotype can be suppressed by the loss of basolateral polarity proteins, suggesting that Cysts is an integral component of the apical polarity protein network. We demonstrate that Cysts is recruited to the apico-lateral membrane through interactions with the Crumbs complex and Bazooka/Par3. Cysts activates Rho1 at adherens junctions and stabilizes junctional myosin. Junctional myosin depletion is similar in Cysts- and Crumbs-compromised embryos. Together, our findings indicate that Cysts is a downstream effector of the Crumbs complex and links apical polarity proteins to Rho1 and myosin activation at adherens junctions, supporting junctional integrity and epithelial polarity.

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