Identification of Maize CC-Type Glutaredoxins That Are Associated with Response to Drought Stress

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2019 Aug 15

PMID 31409044

Citations 7

Authors

Shuangcheng Ding

Fengyu He

Wenlin Tang

Hewei Du

Hongwei Wang

Affiliations

Soon will be listed here.

Abstract

Global maize cultivation is often adversely affected by drought stress. The CC-type glutaredoxin (GRX) genes form a plant-specific subfamily that regulate plant growth and respond to environmental stresses. However, how maize CC-type GRX (ZmGRXCC) genes respond to drought stress remains unclear. We performed a TBLASTN search to identify ZmGRXCCs in the maize genome and verified the identified sequences using the NCBI conservative domain database (CDD). We further established a phylogenetic tree using Mega7 and surveyed known cis-elements in the promoters of ZmGRXCCs using the PlantCARE database. We found twenty-one ZmGRXCCs in the maize genome by a genome-wide investigation and compared their phylogenetic relationships with rice, maize, and Arabidopsis. The analysis of their redox active sites showed that most of the 21 ZmGRXCCs share similar structures with their homologs. We assessed their expression at young seedlings and adult leaves under drought stress and their expression profiles in 15 tissues, and found that they were differentially expressed, indicating that different ZmGRXCC genes have different functions. Notably, ZmGRXCC14 is up-regulated at seedling, V12, V14, V16, and R1 stages. Importantly, significant associations between genetic variation in ZmGRXCC14 and drought tolerance are found at the seedling stage. These results will help to advance the study of the function of ZmGRXCCs genes under drought stress and understand the mechanism of drought resistance in maize.

Citing Articles

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References

Hobo T, Asada M, Kowyama Y, Hattori T . ACGT-containing abscisic acid response element (ABRE) and coupling element 3 (CE3) are functionally equivalent. Plant J. 1999; 19(6):679-89. DOI: 10.1046/j.1365-313x.1999.00565.x. View

Lescot M, Dehais P, Thijs G, Marchal K, Moreau Y, Van de Peer Y . PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences. Nucleic Acids Res. 2001; 30(1):325-7. PMC: 99092. DOI: 10.1093/nar/30.1.325. View

Mittler R . Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 2002; 7(9):405-10. DOI: 10.1016/s1360-1385(02)02312-9. View

Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K . Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell. 2003; 15(1):63-78. PMC: 143451. DOI: 10.1105/tpc.006130. View

Apel K, Hirt H . Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol. 2004; 55:373-99. DOI: 10.1146/annurev.arplant.55.031903.141701. View

Yamaguchi-Shinozaki K, Shinozaki K . Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends Plant Sci. 2005; 10(2):88-94. DOI: 10.1016/j.tplants.2004.12.012. View

Xing S, Rosso M, Zachgo S . ROXY1, a member of the plant glutaredoxin family, is required for petal development in Arabidopsis thaliana. Development. 2005; 132(7):1555-65. DOI: 10.1242/dev.01725. View

Yu J, Pressoir G, Briggs W, Vroh Bi I, Yamasaki M, Doebley J . A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. Nat Genet. 2005; 38(2):203-8. DOI: 10.1038/ng1702. View

Rouhier N, Couturier J, Jacquot J . Genome-wide analysis of plant glutaredoxin systems. J Exp Bot. 2006; 57(8):1685-96. DOI: 10.1093/jxb/erl001. View

10.

Carroll M, Outten C, Proescher J, Rosenfeld L, Watson W, Whitson L . The effects of glutaredoxin and copper activation pathways on the disulfide and stability of Cu,Zn superoxide dismutase. J Biol Chem. 2006; 281(39):28648-56. PMC: 2757158. DOI: 10.1074/jbc.M600138200. View

11.

Kanda M, Ihara Y, Murata H, Urata Y, Kono T, Yodoi J . Glutaredoxin modulates platelet-derived growth factor-dependent cell signaling by regulating the redox status of low molecular weight protein-tyrosine phosphatase. J Biol Chem. 2006; 281(39):28518-28. DOI: 10.1074/jbc.M604359200. View

12.

Bradbury P, Zhang Z, Kroon D, Casstevens T, Ramdoss Y, Buckler E . TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics. 2007; 23(19):2633-5. DOI: 10.1093/bioinformatics/btm308. View

13.

LARKIN M, Blackshields G, Brown N, Chenna R, McGettigan P, McWilliam H . Clustal W and Clustal X version 2.0. Bioinformatics. 2007; 23(21):2947-8. DOI: 10.1093/bioinformatics/btm404. View

14.

Xing S, Zachgo S . ROXY1 and ROXY2, two Arabidopsis glutaredoxin genes, are required for anther development. Plant J. 2007; 53(5):790-801. DOI: 10.1111/j.1365-313X.2007.03375.x. View

15.

Cruz de Carvalho M . Drought stress and reactive oxygen species: Production, scavenging and signaling. Plant Signal Behav. 2009; 3(3):156-65. PMC: 2634109. DOI: 10.4161/psb.3.3.5536. View

16.

Wang Z, Xing S, Birkenbihl R, Zachgo S . Conserved functions of Arabidopsis and rice CC-type glutaredoxins in flower development and pathogen response. Mol Plant. 2009; 2(2):323-35. DOI: 10.1093/mp/ssn078. View

17.

Schnable P, Ware D, Fulton R, Stein J, Wei F, Pasternak S . The B73 maize genome: complexity, diversity, and dynamics. Science. 2009; 326(5956):1112-5. DOI: 10.1126/science.1178534. View

18.

Ziemann M, Bhave M, Zachgo S . Origin and diversification of land plant CC-type glutaredoxins. Genome Biol Evol. 2010; 1:265-77. PMC: 2817420. DOI: 10.1093/gbe/evp025. View

19.

Gill S, Tuteja N . Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem. 2010; 48(12):909-30. DOI: 10.1016/j.plaphy.2010.08.016. View

20.

Garg R, Jhanwar S, Tyagi A, Jain M . Genome-wide survey and expression analysis suggest diverse roles of glutaredoxin gene family members during development and response to various stimuli in rice. DNA Res. 2010; 17(6):353-67. PMC: 2993539. DOI: 10.1093/dnares/dsq023. View