Coxsackievirus B Persistence Modifies the Proteome and the Secretome of Pancreatic Ductal Cells

Overview

Journal iScience

Publisher Cell Press

Date 2019 Aug 13

PMID 31404834

Citations 14

Authors

Niina Lietzen

Karoliina Hirvonen

Anni Honkimaa

Tanja Buchacher

Jutta E Laiho

Sami Oikarinen

Magdalena A Mazur

Malin Flodstrom-Tullberg

Eric Dufour

Amir-Babak Sioofy-Khojine

Heikki Hyoty

Riitta Lahesmaa

Affiliations

Soon will be listed here.

Abstract

The group B Coxsackieviruses (CVB), belonging to the Enterovirus genus, can establish persistent infections in human cells. These persistent infections have been linked to chronic diseases including type 1 diabetes. Still, the outcomes of persistent CVB infections in human pancreas are largely unknown. We established persistent CVB infections in a human pancreatic ductal-like cell line PANC-1 using two distinct CVB1 strains and profiled infection-induced changes in cellular protein expression and secretion using mass spectrometry-based proteomics. Persistent infections, showing characteristics of carrier-state persistence, were associated with a broad spectrum of changes, including changes in mitochondrial network morphology and energy metabolism and in the regulated secretory pathway. Interestingly, the expression of antiviral immune response proteins, and also several other proteins, differed clearly between the two persistent infections. Our results provide extensive information about the protein-level changes induced by persistent CVB infection and the potential virus-associated variability in the outcomes of these infections.

Citing Articles

Serum APOC1 levels are decreased in young autoantibody positive children who rapidly progress to type 1 diabetes.

Hirvonen M, Lietzen N, Moulder R, Bhosale S, Koskenniemi J, Vaha-Makila M Sci Rep. 2023; 13(1):15941.

PMID: 37743383 PMC: 10518308. DOI: 10.1038/s41598-023-43039-4.

Persistent Coxsackievirus B3 Infection in Pancreatic Ductal Cells Downregulates Cellular Polyamine Metabolism.

Mastrodomenico V, LoMascolo N, Firpo M, Del Mar Villanueva Guzman M, Zaporowski A, Mounce B mSphere. 2023; 8(3):e0003623.

PMID: 37097178 PMC: 10286709. DOI: 10.1128/msphere.00036-23.

Persistent coxsackievirus B infection and pathogenesis of type 1 diabetes mellitus.

Nekoua M, Alidjinou E, Hober D Nat Rev Endocrinol. 2022; 18(8):503-516.

PMID: 35650334 PMC: 9157043. DOI: 10.1038/s41574-022-00688-1.

Pancreas Whole Tissue Transcriptomics Highlights the Role of the Exocrine Pancreas in Patients With Recently Diagnosed Type 1 Diabetes.

Valikangas T, Lietzen N, Jaakkola M, Krogvold L, Eike M, Kallionpaa H Front Endocrinol (Lausanne). 2022; 13:861985.

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