Prevalence, Genotypic Characteristics and Antibiotic Resistance of From Retail Foods in Bulk in Zhejiang Province, China

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Aug 13

PMID 31402906

Citations 19

Authors

Yunyi Zhang

Shilei Dong

Honghu Chen

Jiancai Chen

Junyan Zhang

Zhen Zhang

Yong Yang

Ziyan Xu

Li Zhan

Lingling Mei

Affiliations

Soon will be listed here.

Abstract

is an important foodborne pathogen causing public concern. A total of 3354 retail foods in bulk were sampled and screened for . Seventy-three (2.2%) samples including 21 ready-to-eat (RTE) foods and 52 raw foods were confirmed positive for . Sushi and salmon sashimi occupied the top two slots in RTE foods with relatively high presence rate of 12.9 and 6.9%, respectively. Meanwhile, was found to be distributed unequally in raw foods; the presence rates in raw meat (3.5%) and poultry (3.8%) were significantly higher than that in raw seafood (1.3%). Notably, was not detected in raw freshwater food. The . isolates belonged to four serotypes, 1/2a, 1/2b, 1/2c, and 4b, with the most prevalent serotype being 1/2a (47.9%). Eighteen sequence types (STs) and eighteen virulence types (VTs) containing four newly assigned VTs (VT180, VT181, VT182, and VT183) were determined via multilocus sequence typing (MLST) and multi-virulence-locus sequence typing (MVLST). Among the 73 isolates, 23 (31.5%) belonged to epidemic clones (ECs) including ECI, ECIV, ECV, ECVI, ECVIII and ECXI among which ECV was predominant. Antibiotic susceptibility tests revealed a high resistance rate (11.0%) to tetracycline. Moreover, we identified the distribution patterns of virulence genes of four pathogenicity islands (LIPI) in isolates. , , , , , genes in LIPI-1 and , , , genes in LIPI-2 were detected in approximately all isolates. The distribution of both LIPI-3 genes and LIPI-4 genes exhibited association with lineage and ST. LIPI-4 genes were present exclusively in ST87 isolates. Relatedness analysis revealed the absence of distinct association between STs, ECs, LIPI-3 and LIPI-4 distribution and specific food groups. This study provided fundamental data for Chinese food safety authorities to grasp the contamination status of in foods, assess the potential risk of this pathogen and further address the safety issue of retail foods in bulk in China.

Citing Articles

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