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Isolation and Characterization of Marine Methanotrophs

Overview
Journal Antonie Van Leeuwenhoek
Publisher Springer
Specialty Microbiology
Date 1988 Jan 1
PMID 3138946
Citations 23
Authors
M E Lidstrom
Affiliations
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Abstract

Four new methane-oxidizing bacteria have been isolated from marine samples taken at the Hyperion sewage outfall, near Los Angeles, CA. These bacteria require NaCl for growth. All exhibit characteristics typical of Type I methanotrophs, except they contain enzyme activities of both the ribulose monophosphate pathway and the serine cycle. All four strains are characterized by rapid growth in liquid culture and on agar plates, and all have temperature optima above 35 degrees C. One strain, chosen for further study, has been shown to maintain broadhost range cloning vectors and is currently being used for genetic studies.

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Multispecies Populations of Methanotrophic and Cultivation of a Likely Dominant Species from the Iheya North Deep-Sea Hydrothermal Field.

Hirayama H, Takaki Y, Abe M, Imachi H, Ikuta T, Miyazaki J Appl Environ Microbiol. 2021; 88(2):e0075821.

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The Bacteriohopanepolyol Inventory of Novel Aerobic Methane Oxidising Bacteria Reveals New Biomarker Signatures of Aerobic Methanotrophy in Marine Systems.

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Vekeman B, Speth D, Wille J, Cremers G, de Vos P, Op den Camp H Microb Ecol. 2016; 72(3):503-9.

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Draft Genome Sequences of Gammaproteobacterial Methanotrophs Isolated from Marine Ecosystems.

Flynn J, Hirayama H, Sakai Y, Dunfield P, Klotz M, Knief C Genome Announc. 2016; 4(1).

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