Effects of Rosiglitazone/PHBV Drug Delivery System on Postoperative Fibrosis in Rabbit Glaucoma Filtration Surgery Model

Overview

Journal Drug Deliv

Specialty Pharmacology

Date 2019 Aug 8

PMID 31389267

Citations 5

Authors

Feng Zhang

Ke Liu

Zheng Pan

Mengdan Cao

Dengming Zhou

Hairong Liu

Yuting Huang

Xuanchu Duan

Affiliations

Soon will be listed here.

Abstract

The aim of this study is to investigate the effects and toxicities of poly(3-hydroxybutyric acid--3-hydroxyvaleric acid) (PHBV)-loading rosiglitazone on preventing scar formation after glaucoma filtration surgery (GFS) in the rabbit model. Rosiglitazone/PHBV drug delivery system was prepared via electrospinning. Release behavior of RSG/PHBV membrane was evaluated by high-performance liquid chromatography. The different concentration membranes were implanted under the conjunctiva of the rabbit's eyes (RSG/PHBV groups). Also, MMC-soaked sponges were placed under the conjunctiva of the eyes (positive group) for 3 min. Intraocular pressures and bleb features were then assessed for 4 weeks postoperative. Bleb sections were stained with HE, Masson's trichrome and α smooth muscle action (αSMA) immunohistochemistry. The protein expression of collagen I, αSMA, and connective tissue growth factor in the bleb area were then quantified. The following results were observed: (1) the concentration of rosiglitazone would not affect the morphology of RSG/PHBV membrane. (2) RSG/PHBV membrane would effective and safety prevent the formation of fibrosis after GFS in the rabbit model. Implantation of RSG/PHBV membrane prevents scar formation after GFS. What's more, it ameliorated toxicity to conjunctiva and cornea compared with the placement of MMC. The RSG/PHBV membrane would be a more effectivity and safer strategy than MMC.

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References

Picht G, Grehn F . Classification of filtering blebs in trabeculectomy: biomicroscopy and functionality. Curr Opin Ophthalmol. 1998; 9(2):2-8. DOI: 10.1097/00055735-199804000-00002. View

Oshima Y, Sakamoto T, Nakamura T, Tahara Y, Goto Y, Ishibashi T . The comparative benefits of glaucoma filtering surgery with an electric-pulse targeted drug delivery system demonstrated in an animal model. Ophthalmology. 1999; 106(6):1140-6. DOI: 10.1016/S0161-6420(99)90249-X. View

Anand N, Khan A . Long-term outcomes of needle revision of trabeculectomy blebs with mitomycin C and 5-fluorouracil: a comparative safety and efficacy report. J Glaucoma. 2009; 18(7):513-20. DOI: 10.1097/IJG.0b013e3181911271. View

Bhardwaj N, Kundu S . Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv. 2010; 28(3):325-47. DOI: 10.1016/j.biotechadv.2010.01.004. View

Seibold L, Sherwood M, Kahook M . Wound modulation after filtration surgery. Surv Ophthalmol. 2012; 57(6):530-50. DOI: 10.1016/j.survophthal.2012.01.008. View

Lockwood A, Brocchini S, Khaw P . New developments in the pharmacological modulation of wound healing after glaucoma filtration surgery. Curr Opin Pharmacol. 2012; 13(1):65-71. DOI: 10.1016/j.coph.2012.10.008. View

Nagy Z, Balogh A, Dravavolgyi G, Ferguson J, Pataki H, Vajna B . Solvent-free melt electrospinning for preparation of fast dissolving drug delivery system and comparison with solvent-based electrospun and melt extruded systems. J Pharm Sci. 2012; 102(2):508-17. DOI: 10.1002/jps.23374. View

Hu J, Wei J, Liu W, Chen Y . Preparation and characterization of electrospun PLGA/gelatin nanofibers as a drug delivery system by emulsion electrospinning. J Biomater Sci Polym Ed. 2013; 24(8):972-85. DOI: 10.1080/09205063.2012.728193. View

Hamori M, Yoshimatsu S, Hukuchi Y, Shimizu Y, Fukushima K, Sugioka N . Preparation and pharmaceutical evaluation of nano-fiber matrix supported drug delivery system using the solvent-based electrospinning method. Int J Pharm. 2014; 464(1-2):243-51. DOI: 10.1016/j.ijpharm.2013.12.036. View

10.

Xi L, Wang T, Zhao F, Zheng Q, Li X, Luo J . Evaluation of an injectable thermosensitive hydrogel as drug delivery implant for ocular glaucoma surgery. PLoS One. 2014; 9(6):e100632. PMC: 4065085. DOI: 10.1371/journal.pone.0100632. View

11.

Luo Y, Ouyang P, Tian J, Guo X, Duan X . Rosiglitazone inhibits TGF-β 1 induced activation of human Tenon fibroblasts via p38 signal pathway. PLoS One. 2014; 9(8):e105796. PMC: 4140818. DOI: 10.1371/journal.pone.0105796. View

12.

Fathollahipour S, Abouei Mehrizi A, Ghaee A, Koosha M . Electrospinning of PVA/chitosan nanocomposite nanofibers containing gelatin nanoparticles as a dual drug delivery system. J Biomed Mater Res A. 2015; 103(12):3852-62. DOI: 10.1002/jbm.a.35529. View

13.

Mei L, Wang Y, Tong A, Guo G . Facile electrospinning of an efficient drug delivery system. Expert Opin Drug Deliv. 2016; 13(5):741-53. DOI: 10.1517/17425247.2016.1142525. View

14.

Penaloza J, Marquez-Miranda V, Cabana-Brunod M, Reyes-Ramirez R, Llancalahuen F, Vilos C . Intracellular trafficking and cellular uptake mechanism of PHBV nanoparticles for targeted delivery in epithelial cell lines. J Nanobiotechnology. 2017; 15(1):1. PMC: 5210312. DOI: 10.1186/s12951-016-0241-6. View

15.

Ozdemir S, Wong T, Allingham R, Finkelstein E . Predicted patient demand for a new delivery system for glaucoma medicine. Medicine (Baltimore). 2017; 96(15):e6626. PMC: 5403112. DOI: 10.1097/MD.0000000000006626. View

16.

Gandhi K, Maganti R, Kaur H, Vinod K, Verma P . Formulation and Evaluation of Sol-Gel Drug Delivery System for Intracanal pH Sensitive Controlled Delivery of Chlorhexidine. J Clin Diagn Res. 2017; 11(4):ZC68-ZC72. PMC: 5449922. DOI: 10.7860/JCDR/2017/26061.9693. View

17.

Vahedian Z, Mafi M, Fakhraie G, Zarei R, Eslami Y, Ghadimi H . Short-term Results of Trabeculectomy Using Adjunctive Intracameral Bevacizumab Versus Mitomycin C: A Randomized Controlled Trial. J Glaucoma. 2017; 26(9):829-834. DOI: 10.1097/IJG.0000000000000741. View

18.

Li H, Liu K, Sang Q, Williams G, Wu J, Wang H . A thermosensitive drug delivery system prepared by blend electrospinning. Colloids Surf B Biointerfaces. 2017; 159:277-283. DOI: 10.1016/j.colsurfb.2017.07.058. View

19.

Gai X, Cheng L, Li T, Liu D, Wang Y, Wang T . In vitro and In vivo Studies on a Novel Bioadhesive Colloidal System: Cationic Liposomes of Ibuprofen. AAPS PharmSciTech. 2017; 19(2):700-709. DOI: 10.1208/s12249-017-0872-4. View

20.

Vicario-de-la-Torre M, Caballo-Gonzalez M, Vico E, Morales-Fernandez L, Arriola-Villalobos P, de Las Heras B . Novel Nano-Liposome Formulation for Dry Eyes with Components Similar to the Preocular Tear Film. Polymers (Basel). 2019; 10(4). PMC: 6415276. DOI: 10.3390/polym10040425. View