Factors Influencing the Activity of Nanozymes in the Cleavage of an RNA Model Substrate

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2019 Aug 4

PMID 31374998

Citations 5

Authors

Joanna Czescik

Susanna Zamolo

Tamis Darbre

Fabrizio Mancin

Paolo Scrimin

Affiliations

Soon will be listed here.

Abstract

A series of 2-nm gold nanoparticles passivated with different thiols all featuring at least one triazacyclonanone-Zn(II) complex and different flanking units (a second Zn(II) complex, a triethyleneoxymethyl derivative or a guanidinium of arginine of a peptide) were prepared and studied for their efficiency in the cleavage of the RNA-model substrate 2-hydroxypropyl--nitrophenyl phosphate. The source of catalysis for each of them was elucidated from the kinetic analysis (Michaelis-Menten profiles, pH dependence and kinetic isotope effect). The data indicated that two different mechanisms were operative: One involving two Zn(II) complexes and the other one involving a single Zn(II) complex and a flanking guanidinium cation. The mechanism based on a dinuclear catalytic site appeared more efficient than the one based on the cooperativity between a metal complex and a guanidinium.

Citing Articles

Nuclease-like metalloscissors: Biomimetic candidates for cancer and bacterial and viral infections therapy.

Anjomshoa M, Amirheidari B Coord Chem Rev. 2022; 458:214417.

PMID: 35153301 PMC: 8816526. DOI: 10.1016/j.ccr.2022.214417.

The Mechanism of Cleavage of RNA Phosphodiesters by a Gold Nanoparticle Nanozyme.

Czescik J, Mancin F, Stromberg R, Scrimin P Chemistry. 2021; 27(31):8143-8148.

PMID: 33780067 PMC: 8251847. DOI: 10.1002/chem.202100299.

A Gold Nanoparticle Nanonuclease Relying on a Zn(II) Mononuclear Complex.

Czescik J, Zamolo S, Darbre T, Rigo R, Sissi C, Pecina A Angew Chem Int Ed Engl. 2020; 60(3):1423-1432.

PMID: 32985766 PMC: 7839518. DOI: 10.1002/anie.202012513.

A Trisbenzimidazole Phosphoramidite Building Block Enables High-Yielding Syntheses of RNA-Cleaving Oligonucleotide Conjugates.

Zellmann F, Gobel M Molecules. 2020; 25(8).

PMID: 32316292 PMC: 7221912. DOI: 10.3390/molecules25081842.

Host-Guest Allosteric Control of an Artificial Phosphatase.

Czescik J, Lyu Y, Neuberg S, Scrimin P, Mancin F J Am Chem Soc. 2020; 142(15):6837-6841.

PMID: 32212681 PMC: 7997383. DOI: 10.1021/jacs.9b12699.

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