Lion's Mane Mushroom, (Bull.: Fr.) Pers. Suppresses HO-Induced Oxidative Damage and LPS-Induced Inflammation in HT22 Hippocampal Neurons and BV2 Microglia

Overview

Journal Antioxidants (Basel)

Date 2019 Aug 4

PMID 31374912

Citations 22

Authors

Naufal Kushairi

Chia Wei Phan

Vikineswary Sabaratnam

Pamela David

Murali Naidu

Affiliations

Soon will be listed here.

Abstract

Oxidative stress and inflammation in neuron-glia system are key factors in the pathogenesis of neurodegenerative diseases. As synthetic drugs may cause side effects, natural products have gained recognition for the prevention or management of diseases. In this study, hot water (HE-HWA) and ethanolic (HE-ETH) extracts of the basidiocarps of mushroom were investigated for their neuroprotective and anti-inflammatory activities against hydrogen peroxide (HO)-induced neurotoxicity in HT22 mouse hippocampal neurons and lipopolysaccharide (LPS)-induced BV2 microglial activation respectively. HE-ETH showed potent neuroprotective activity by significantly ( < 0.0001) increasing the viability of HO-treated neurons. This was accompanied by significant reduction in reactive oxygen species (ROS) ( < 0.05) and improvement of the antioxidant enzyme catalase (CAT) ( < 0.05) and glutathione (GSH) content ( < 0.01). Besides, HE-ETH significantly improved mitochondrial membrane potential (MMP) ( < 0.05) and ATP production ( < 0.0001) while reducing mitochondrial toxicity ( < 0.001), Bcl-2-associated X (Bax) gene expression ( < 0.05) and nuclear apoptosis ( < 0.0001). However, gene expression of Nuclear factor erythroid 2-related factor 2 (Nrf2), heme oxygenase 1 (HO-1) and NAD(P)H quinone dehydrogenase 1 (NQO1) were unaffected ( > 0.05). HE-ETH also significantly ( < 0.0001) reduced nitric oxide (NO) level in LPS-treated BV2 indicating an anti-inflammatory activity in the microglia. These findings demonstrated HE-ETH maybe a potential neuroprotective and anti-inflammatory agent in neuron-glia environment.

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References

Maines M . Heme oxygenase: function, multiplicity, regulatory mechanisms, and clinical applications. FASEB J. 1988; 2(10):2557-68. View

Lee E, Shizuki K, Hosokawa S, Suzuki M, Suganuma H, Inakuma T . Two novel diterpenoids, erinacines H and I from the mycelia of Hericium erinaceum. Biosci Biotechnol Biochem. 2001; 64(11):2402-5. DOI: 10.1271/bbb.64.2402. View

Bergamini C, Gambetti S, Dondi A, Cervellati C . Oxygen, reactive oxygen species and tissue damage. Curr Pharm Des. 2004; 10(14):1611-26. DOI: 10.2174/1381612043384664. View

Xu N, Gao Z, Zhang J, Jing H, Li S, Ren Z . Hepatoprotection of enzymatic-extractable mycelia zinc polysaccharides by Pleurotus eryngii var. tuoliensis. Carbohydr Polym. 2016; 157:196-206. DOI: 10.1016/j.carbpol.2016.09.082. View

Hroudova J, Singh N, Fisar Z . Mitochondrial dysfunctions in neurodegenerative diseases: relevance to Alzheimer's disease. Biomed Res Int. 2014; 2014:175062. PMC: 4036420. DOI: 10.1155/2014/175062. View

Kim D, Li H, Han Y, Jeong J, Lee H, Ryu J . Modulation of Inducible Nitric Oxide Synthase Expression in LPS-Stimulated BV-2 Microglia by Prenylated Chalcones from Cullen corylifolium (L.) Medik. through Inhibition of I-κBα Degradation. Molecules. 2018; 23(1). PMC: 6017879. DOI: 10.3390/molecules23010109. View

Zhang Z, Lv G, Pan H, Pandey A, He W, Fan L . Antioxidant and hepatoprotective potential of endo-polysaccharides from Hericium erinaceus grown on tofu whey. Int J Biol Macromol. 2012; 51(5):1140-6. DOI: 10.1016/j.ijbiomac.2012.09.002. View

Ames B, Shigenaga M, Hagen T . Oxidants, antioxidants, and the degenerative diseases of aging. Proc Natl Acad Sci U S A. 1993; 90(17):7915-22. PMC: 47258. DOI: 10.1073/pnas.90.17.7915. View

Giannenas I, Pappas I, Mavridis S, Kontopidis G, Skoufos J, Kyriazakis I . Performance and antioxidant status of broiler chickens supplemented with dried mushrooms (Agaricus bisporus) in their diet. Poult Sci. 2010; 89(2):303-11. DOI: 10.3382/ps.2009-00207. View

10.

Karmarkar S, Bottum K, Krager S, Tischkau S . ERK/MAPK is essential for endogenous neuroprotection in SCN2.2 cells. PLoS One. 2011; 6(8):e23493. PMC: 3157406. DOI: 10.1371/journal.pone.0023493. View

11.

Lim J, Wilhelmus M, de Vries H, Drukarch B, Hoozemans J, van Horssen J . Antioxidative defense mechanisms controlled by Nrf2: state-of-the-art and clinical perspectives in neurodegenerative diseases. Arch Toxicol. 2014; 88(10):1773-86. DOI: 10.1007/s00204-014-1338-z. View

12.

Li Q, Wu D, Chen X, Zhou S, Liu Y, Yang Y . Chemical Compositions and Macrophage Activation of Polysaccharides from Leon's Mane Culinary-Medicinal Mushroom Hericium erinaceus (Higher Basidiomycetes) in Different Maturation Stages. Int J Med Mushrooms. 2015; 17(5):443-52. DOI: 10.1615/intjmedmushrooms.v17.i5.40. View

13.

Cheng J, Tsai C, Lien Y, Lee M, Sheu S . High molecular weight of polysaccharides from Hericium erinaceus against amyloid beta-induced neurotoxicity. BMC Complement Altern Med. 2016; 16:170. PMC: 4895996. DOI: 10.1186/s12906-016-1154-5. View

14.

Dai S, Chen T, Wang Y, Zhu J, Luo P, Rao W . Sirt3 attenuates hydrogen peroxide-induced oxidative stress through the preservation of mitochondrial function in HT22 cells. Int J Mol Med. 2014; 34(4):1159-68. DOI: 10.3892/ijmm.2014.1876. View

15.

Kobayashi A, Kang M, Okawa H, Ohtsuji M, Zenke Y, Chiba T . Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2. Mol Cell Biol. 2004; 24(16):7130-9. PMC: 479737. DOI: 10.1128/MCB.24.16.7130-7139.2004. View

16.

Wang X, Sun Z, Villeneuve N, Zhang S, Zhao F, Li Y . Nrf2 enhances resistance of cancer cells to chemotherapeutic drugs, the dark side of Nrf2. Carcinogenesis. 2008; 29(6):1235-43. PMC: 3312612. DOI: 10.1093/carcin/bgn095. View

17.

Hwang G, Jeon Y, Han H, Park S, Baek K, Chang W . Protective effect of butylated hydroxylanisole against hydrogen peroxide-induced apoptosis in primary cultured mouse hepatocytes. J Vet Sci. 2015; 16(1):17-23. PMC: 4367145. DOI: 10.4142/jvs.2015.16.1.17. View

18.

Jha S, Jha N, Kar R, Ambasta R, Kumar P . p38 MAPK and PI3K/AKT Signalling Cascades inParkinson's Disease. Int J Mol Cell Med. 2015; 4(2):67-86. PMC: 4499569. View

19.

Anandhi R, Annadurai T, Anitha T, Muralidharan A, Najmunnisha K, Nachiappan V . Antihypercholesterolemic and antioxidative effects of an extract of the oyster mushroom, Pleurotus ostreatus, and its major constituent, chrysin, in Triton WR-1339-induced hypercholesterolemic rats. J Physiol Biochem. 2012; 69(2):313-23. DOI: 10.1007/s13105-012-0215-6. View

20.

Phan C, Lee G, Hong S, Wong Y, Brkljaca R, Urban S . Hericium erinaceus (Bull.: Fr) Pers. cultivated under tropical conditions: isolation of hericenones and demonstration of NGF-mediated neurite outgrowth in PC12 cells via MEK/ERK and PI3K-Akt signaling pathways. Food Funct. 2014; 5(12):3160-9. DOI: 10.1039/c4fo00452c. View