Effect of Plateletpheresis on Postdonation Serum Thrombopoietin Levels and Its Correlation with Platelet Counts in Healthy Voluntary Donors

Overview

Journal Asian J Transfus Sci

Specialty Hematology

Date 2019 Jul 31

PMID 31360004

Authors

Rekha Hans

Ratti Ram Sharma

Neelam Marwaha

Affiliations

Soon will be listed here.

Abstract

Background: Thrombopoietin (TPO) is regulated by a feedback mechanism between megakaryocytes and platelets. This is important in plateletpheresis donors to compensate for donation-associated platelet loss.

Aims And Objectives: The aim and objective of this study were to investigate changes in serum TPO levels in healthy plateletpheresis donors and its correlation with platelet recovery pattern.

Materials And Methods: Out of 50 plateletpheresis donors recruited in the study over 1 year, only 29 completed follow-up and were further analyzed. Plateletpheresis procedures were performed on two types of cell separators (TRIMA ACCEL, Terumo BCT Lakewood Colorado and AMICUS, Fresenius Kabi, Germany). Platelet parameters were estimated pre- and post-platelet donation, at 3- and 5-day postdonation. Serum TPO levels were determined using quantitative sandwich enzyme-linked immunosorbent assay technique (Raybiotech, USA) as per the protocol of the manufacturer.

Results: The majority of donors (72%) in our study were first-time donors. The baseline platelet count was 226 ± 44 × 10/μl with a significant decline (30%; < 0.001) in postdonation phase and remained below baseline on the 3 and 5 day. The serum TPO levels increased significantly ( < 0.001) from a baseline of 227.81 (interquartile range [IQR]: 176.06) pg/ml to 269.94 (IQR: 110.68) pg/ml postdonation and remained elevated from baseline levels on the 3 and 5 day. An inverse relation was observed between change in serum TPO levels and platelet count during postdonation phase which was not statistically significant ( > 0.05).

Conclusion: Serum TPO levels increase significantly post plateletpheresis donation corresponding to decrease in platelet counts showing that TPO plays a vital role in compensatory mechanism after platelet loss.

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