Interactions of Bacteria With Monolithic Lateral Silicon Nanospikes Inside a Microfluidic Channel

Overview

Journal Front Chem

Specialty Chemistry

Date 2019 Jul 30

PMID 31355180

Citations 8

Authors

Lei Li

Feng Tian

Hao Chang

Jie Zhang

Cheng Wang

Wei Rao

Huan Hu

Affiliations

Soon will be listed here.

Abstract

This paper presents a new strategy of integrating lateral silicon nanospikes using metal-assisted chemical etching (MacEtch) on the sidewall of micropillars for on-chip bacterial study. Silicon nanospikes have been reported to be able to kill bacteria without using chemicals and offer a new route to kill bacteria and can prevent the overuse of antibiotics to reduce bacteria. We demonstrated a new methodology to fabricate a chip with integrated silicon nanospikes onto the sidewalls of micropillars inside the microfluidic channel and attested its interactions with the representative gram-negative bacteria . The results of colony-forming unit (CFU) calculation showed that 80% bacteria lost their viability after passing through the chip. Moreover, the results of adenosine triphosphate (ATP) measurement indicated that the chip with lateral silicon nanospikes could extract more than two times ATP contents compared with the chip without lateral silicon nanospikes, showing potential for using the chip with lateral silicon nanospikes as a bacterial lysing module.

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