Carotenoid-to-(bacterio)chlorophyll Energy Transfer in LH2 Antenna Complexes from Rba. Sphaeroides Reconstituted with Non-native (bacterio)chlorophylls

Overview

Journal Photosynth Res

Publisher Springer

Date 2019 Jul 28

PMID 31350671

Citations 2

Authors

Dariusz M Niedzwiedzki

David J K Swainsbury

C Neil Hunter

Affiliations

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Abstract

Six variants of the LH2 antenna complex from Rba. sphaeroides, comprising the native B800-B850, B800-free LH2 (B850) and four LH2s with various (bacterio)chlorophylls reconstituted into the B800 site, have been investigated with static and time-resolved optical spectroscopies at room temperature and at 77 K. The study particularly focused on how reconstitution of a non-native (bacterio)chlorophylls affects excitation energy transfer between the naturally bound carotenoid spheroidene and artificially substituted pigments in the B800 site. Results demonstrate there is no apparent trend in the overall energy transfer rate from spheroidene to B850 bacteriochlorophyll a; however, a trend in energy transfer rate from the spheroidene S state to Q of the B800 (bacterio)chlorophylls is noticeable. These outcomes were applied to test the validity of previously proposed energy values of the spheroidene S state, supporting a value in the vicinity of 13,400 cm (746 nm).

Citing Articles

Excitation Energy Transfer Dynamics from Carotenoid to Bacteriochlorophyll in the LH2 Complex of : Insights from Reconstitution Experiments with Carotenoids and B800 Bacteriochlorophyll .

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Chlorophyll Synthesis in by Chlorophyll Synthase of .

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Reconstitution of 3-Acetyl Chlorophyll into Light-Harvesting Complex 2 from the Purple Photosynthetic Bacterium .

Saga Y, Yamashita M, Imanishi M, Kimura Y, Masaoka Y, Hidaka T ACS Omega. 2020; 5(12):6817-6825.

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