Passive Filtration, Rapid Scanning Electron Microscopy, and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Culture and Identification from the Oral Cavity

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2019 Jul 26

PMID 31340994

Citations 10

Authors

Souad Belkacemi

Jacques Bou Khalil

Yusuke Ominami

Akiko Hisada

Anthony Fontanini

Aurelia Caputo

Anthony Levasseur

Jean-Christophe Lagier

Saber Khelaifia

Didier Raoult

Affiliations

Soon will be listed here.

Abstract

We present here a new passive-filtration-based culture device combined with rapid identification with a new electron microscope (Hitachi TM4000) for the detection and culture of species from the human oral cavity. Of the 44 oral samples cultivated, 15 (34%) were found to be positive for using electron microscopy and were also culture positive. All were subcultured on agar plates; based on genome sequencing and analyses, 10 were strains of and 5 were strains of The 29 samples that were negative for remained culture negative. In addition, 14 species ordered from the DSMZ collection were cultured in the T-Raoult culture medium optimized here. Finally, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used and 30 novel spectra were added to the MALDI-TOF MS database. We have successfully developed a new and effective method for treponemal detection, culture, and identification.

Citing Articles

Maliibacterium massiliense gen. nov. sp. nov., Isolated from Human Feces and Proposal of Maliibacteriaceae fam. nov.

Traore S, Lo C, Mossaab M, Durand G, Lagier J, Raoult D Curr Microbiol. 2023; 80(6):211.

PMID: 37191823 DOI: 10.1007/s00284-023-03301-4.

Culturing the Human Oral Microbiota, Updating Methodologies and Cultivation Techniques.

Khelaifia S, Virginie P, Belkacemi S, Tassery H, Terrer E, Aboudharam G Microorganisms. 2023; 11(4).

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Immunohistochemical diagnosis of human infectious diseases: a review.

Oumarou Hama H, Aboudharam G, Barbieri R, Lepidi H, Drancourt M Diagn Pathol. 2022; 17(1):17.

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Bhargavaea massiliensis sp. nov. and Dietzia massiliensis sp. nov., Novel Bacteria Species Isolated from Human Urine Samples in Nigeria.

Olowo-Okere A, Ibrahim Y, Lo C, Olayinka B, Kuete Yimagou E, Yacouba A Curr Microbiol. 2021; 79(1):18.

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Taxonogenomics description of sp. nov., a new species isolated from the stool sample.

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PMID: 34188938 PMC: 8220230. DOI: 10.1016/j.nmni.2021.100906.

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