Chalcone Isomerase a Key Enzyme for Anthocyanin Biosynthesis in

Overview

Journal Front Plant Sci

Date 2019 Jul 25

PMID 31338101

Citations 27

Authors

Wei Sun

Huan Shen

Hui Xu

Xiaoxin Tang

Ming Tang

Zhigang Ju

Yin Yi

Affiliations

Soon will be listed here.

Abstract

Anthocyanins are distributed ubiquitously to terrestrial plants and chalcone isomerase (CHI) catalyzes the stereospecific isomerization of chalcones - a committed step in the anthocyanin biosynthesis pathway. In this study, one gene encoding CHI was isolated from and designated as . Multiple sequence alignments and phylogenetic analysis revealed that OjCHI had the conserved CHI active site residues and was classified into type I CHI group. In order to better understand the mechanisms of anthocyanin synthesis in , integrative analysis between metabolites and expression was conducted. The results showed expression matched the accumulation patterns of anthocyanins not only in different tissues but also during the flower developmental stages, suggesting the potential roles of in the biosynthesis of anthocyanin. Then biochemical analysis indicated that recombinant OjCHI protein exhibited a typical type I CHI activity which catalyzed the production of naringenin from naringenin chalcone. Moreover, expressing in Arabidopsis mutant restored the anthocyanins and flavonols phenotype of hypocotyl, cotyledon and seed coat, indicating its function as a chalcone isomerase . In summary, our findings reveal the as well as functions of OjCHI and provide a resource to understand the mechanism of anthocyanin biosynthesis in .

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References

Clough S, Bent A . Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J. 1999; 16(6):735-43. DOI: 10.1046/j.1365-313x.1998.00343.x. View

Jez J, Bowman M, Dixon R, Noel J . Structure and mechanism of the evolutionarily unique plant enzyme chalcone isomerase. Nat Struct Biol. 2000; 7(9):786-91. DOI: 10.1038/79025. View

. Flavonoid biosynthesis. A colorful model for genetics, biochemistry, cell biology, and biotechnology. Plant Physiol. 2001; 126(2):485-93. PMC: 1540115. DOI: 10.1104/pp.126.2.485. View

Dong X, Braun E, Grotewold E . Functional conservation of plant secondary metabolic enzymes revealed by complementation of Arabidopsis flavonoid mutants with maize genes. Plant Physiol. 2001; 127(1):46-57. PMC: 117961. DOI: 10.1104/pp.127.1.46. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Jez J, Bowman M, Noel J . Role of hydrogen bonds in the reaction mechanism of chalcone isomerase. Biochemistry. 2002; 41(16):5168-76. DOI: 10.1021/bi0255266. View

Liu C, Blount J, Steele C, Dixon R . Bottlenecks for metabolic engineering of isoflavone glycoconjugates in Arabidopsis. Proc Natl Acad Sci U S A. 2002; 99(22):14578-83. PMC: 137925. DOI: 10.1073/pnas.212522099. View

Druka A, Kudrna D, Rostoks N, Brueggeman R, von Wettstein D, Kleinhofs A . Chalcone isomerase gene from rice (Oryza sativa) and barley (Hordeum vulgare): physical, genetic and mutation mapping. Gene. 2003; 302(1-2):171-8. DOI: 10.1016/s0378-1119(02)01105-8. View

Shimada N, Aoki T, Sato S, Nakamura Y, Tabata S, Ayabe S . A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxy(iso)flavonoids in Lotus japonicus. Plant Physiol. 2003; 131(3):941-51. PMC: 166860. DOI: 10.1104/pp.004820. View

10.

Kong J, Chia L, Goh N, Chia T, Brouillard R . Analysis and biological activities of anthocyanins. Phytochemistry. 2003; 64(5):923-33. DOI: 10.1016/s0031-9422(03)00438-2. View

11.

Bradshaw H, Schemske D . Allele substitution at a flower colour locus produces a pollinator shift in monkeyflowers. Nature. 2003; 426(6963):176-8. DOI: 10.1038/nature02106. View

12.

Konczak I, Zhang W . Anthocyanins-More Than Nature's Colours. J Biomed Biotechnol. 2004; 2004(5):239-240. PMC: 1082903. DOI: 10.1155/S1110724304407013. View

13.

Ralston L, Subramanian S, Matsuno M, Yu O . Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast using soybean type I and type II chalcone isomerases. Plant Physiol. 2005; 137(4):1375-88. PMC: 1088328. DOI: 10.1104/pp.104.054502. View

14.

Nishihara M, Nakatsuka T, Yamamura S . Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene. FEBS Lett. 2005; 579(27):6074-8. DOI: 10.1016/j.febslet.2005.09.073. View

15.

Grotewold E . The genetics and biochemistry of floral pigments. Annu Rev Plant Biol. 2006; 57:761-80. DOI: 10.1146/annurev.arplant.57.032905.105248. View

16.

Li F, Jin Z, Qu W, Zhao D, Ma F . Cloning of a cDNA encoding the Saussurea medusa chalcone isomerase and its expression in transgenic tobacco. Plant Physiol Biochem. 2006; 44(7-9):455-61. DOI: 10.1016/j.plaphy.2006.08.006. View

17.

Blyden E, Doerner P, Lamb C, Dixon R . Sequence analysis of a chalcone isomerase cDNA of Phaseolus vulgaris L. Plant Mol Biol. 1991; 16(1):167-9. DOI: 10.1007/BF00017927. View

18.

Cheng H, Li L, Cheng S, Cao F, Wang Y, Yuan H . Molecular cloning and function assay of a chalcone isomerase gene (GbCHI) from Ginkgo biloba. Plant Cell Rep. 2010; 30(1):49-62. DOI: 10.1007/s00299-010-0943-4. View

19.

Fanali C, Dugo L, DOrazio G, Lirangi M, Dacha M, Dugo P . Analysis of anthocyanins in commercial fruit juices by using nano-liquid chromatography-electrospray-mass spectrometry and high-performance liquid chromatography with UV-vis detector. J Sep Sci. 2011; 34(2):150-9. DOI: 10.1002/jssc.201000665. View

20.

Petroni K, Tonelli C . Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Sci. 2011; 181(3):219-29. DOI: 10.1016/j.plantsci.2011.05.009. View