Effects of Freeze-Thaw Event on Microbial Community Dynamics During Red Clover Ensiling

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Jul 25

PMID 31338085

Citations 18

Authors

Zhihao Dong

Junfeng Li

Lei Chen

Siran Wang

Tao Shao

Affiliations

Soon will be listed here.

Abstract

Freezing damages in forages represents a major economic loss to agriculture. This study was conducted to investigate the effects of freeze-thaw (FT) event on microbial community dynamics of red clover silage. Results showed that the FT-treated material displayed higher proportions of and aerobic bacteria, while lower and compared with the control material. The FT event promoted the development of in silage microflora, inducing more intense lactic fermentation after an initial short lag. The aerobic bacteria were suppressed immediately after the onset of ensiling. Microbiomes of the two silages tended to be almost similar after 2 days of ensiling. However, a small number of aerobic bacteria tended to revitalize in the FT silage with prolonged ensiling time, indicated by apparent abundances of and at the end of ensiling. The results obtained here suggest that the FT event could promote the development of during ensiling and the control of aerobe revitalization need to be concerned with silages made from the freeze-damaged forages.

Citing Articles

Effects of temperature and lactic acid Bacteria additives on the quality and microbial community of wilted alfalfa silage.

Liu J, Zhao M, Hao J, Yan X, Fu Z, Zhu N BMC Plant Biol. 2024; 24(1):844.

PMID: 39251915 PMC: 11382506. DOI: 10.1186/s12870-024-05501-x.

Study on Dynamic Fermentation of Oat Silage Assisted by Exogenous Fibrolytic Enzymes.

Liu W, Du S, Sun L, Wang Z, Ge G, Jia Y Plants (Basel). 2024; 13(1).

PMID: 38202317 PMC: 10780392. DOI: 10.3390/plants13010006.

Effects of Tannin Extract as an Additive on Fermentation Quality, Aerobic Stability, and Microbial Modulation of Maize Silage.

Mpanza T, Mani S Microorganisms. 2023; 11(11).

PMID: 38004778 PMC: 10673101. DOI: 10.3390/microorganisms11112767.

Dynamics of Phyllosphere Microbiota and Chemical Parameters at Various Growth Stages and Their Contribution to Anaerobic Fermentation of .

Zhao J, Liu H, Yin X, Dong Z, Wang S, Li J Microbiol Spectr. 2023; 11(3):e0228822.

PMID: 37010418 PMC: 10269755. DOI: 10.1128/spectrum.02288-22.

Evaluating the fermentation characteristics, bacterial community, and predicted functional profiles of native grass ensiled with different additives.

Du S, You S, Jiang X, Li Y, Wang R, Ge G Front Microbiol. 2022; 13:1025536.

PMID: 36329844 PMC: 9623271. DOI: 10.3389/fmicb.2022.1025536.

References

Ercolini D . PCR-DGGE fingerprinting: novel strategies for detection of microbes in food. J Microbiol Methods. 2004; 56(3):297-314. DOI: 10.1016/j.mimet.2003.11.006. View

Stevenson D, Muck R, Shinners K, Weimer P . Use of real time PCR to determine population profiles of individual species of lactic acid bacteria in alfalfa silage and stored corn stover. Appl Microbiol Biotechnol. 2005; 71(3):329-38. DOI: 10.1007/s00253-005-0170-z. View

Palleroni N . The Pseudomonas story. Environ Microbiol. 2010; 12(6):1377-83. DOI: 10.1111/j.1462-2920.2009.02041.x. View

Li Y, Nishino N . Bacterial and fungal communities of wilted Italian ryegrass silage inoculated with and without Lactobacillus rhamnosus or Lactobacillus buchneri. Lett Appl Microbiol. 2011; 52(4):314-21. DOI: 10.1111/j.1472-765X.2010.03000.x. View

Pang H, Qin G, Tan Z, Li Z, Wang Y, Cai Y . Natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype, 16S ribosomal RNA and recA gene analysis. Syst Appl Microbiol. 2011; 34(3):235-41. DOI: 10.1016/j.syapm.2010.10.003. View

Papagianni M, Papamichael E . Purification, amino acid sequence and characterization of the class IIa bacteriocin weissellin A, produced by Weissella paramesenteroides DX. Bioresour Technol. 2011; 102(12):6730-4. DOI: 10.1016/j.biortech.2011.03.106. View

Kampfer P, Glaeser S . Prokaryotic taxonomy in the sequencing era--the polyphasic approach revisited. Environ Microbiol. 2011; 14(2):291-317. DOI: 10.1111/j.1462-2920.2011.02615.x. View

McGarvey J, Franco R, Palumbo J, Hnasko R, Stanker L, Mitloehner F . Bacterial population dynamics during the ensiling of Medicago sativa (alfalfa) and subsequent exposure to air. J Appl Microbiol. 2013; 114(6):1661-70. DOI: 10.1111/jam.12179. View

Fuhs G, Chen M . Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater. Microb Ecol. 2013; 2(2):119-38. DOI: 10.1007/BF02010434. View

10.

Graf K, Ulrich A, Idler C, Klocke M . Bacterial community dynamics during ensiling of perennial ryegrass at two compaction levels monitored by terminal restriction fragment length polymorphism. J Appl Microbiol. 2016; 120(6):1479-91. DOI: 10.1111/jam.13114. View

11.

Bertrand A, Bipfubusa M, Castonguay Y, Rocher S, Szopinska-Morawska A, Papadopoulos Y . A proteome analysis of freezing tolerance in red clover (Trifolium pratense L.). BMC Plant Biol. 2016; 16:65. PMC: 4787020. DOI: 10.1186/s12870-016-0751-2. View

12.

Carrion C, Martinez D, Costa M, Guiamet J . Senescence-Associated Vacuoles, a Specific Lytic Compartment for Degradation of Chloroplast Proteins?. Plants (Basel). 2016; 3(4):498-512. PMC: 4844279. DOI: 10.3390/plants3040498. View

13.

Ni K, Minh T, Tu T, Tsuruta T, Pang H, Nishino N . Comparative microbiota assessment of wilted Italian ryegrass, whole crop corn, and wilted alfalfa silage using denaturing gradient gel electrophoresis and next-generation sequencing. Appl Microbiol Biotechnol. 2016; 101(4):1385-1394. DOI: 10.1007/s00253-016-7900-2. View

14.

Phalakornkule C, Nuchdang S, Khemkhao M, Mhuantong W, Wongwilaiwalin S, Tangphatsornruang S . Effect of freeze-thaw process on physical properties, microbial activities and population structures of anaerobic sludge. J Biosci Bioeng. 2016; 123(4):474-481. DOI: 10.1016/j.jbiosc.2016.11.005. View

15.

Zheng M, Niu D, Jiang D, Zuo S, Xu C . Dynamics of microbial community during ensiling direct-cut alfalfa with and without LAB inoculant and sugar. J Appl Microbiol. 2017; 122(6):1456-1470. DOI: 10.1111/jam.13456. View

16.

Dong Z, Yuan X, Wen A, Desta S, Shao T . Effects of calcium propionate on the fermentation quality and aerobic stability of alfalfa silage. Asian-Australas J Anim Sci. 2017; 30(9):1278-1284. PMC: 5582284. DOI: 10.5713/ajas.16.0956. View

17.

Ni K, Wang F, Zhu B, Yang J, Zhou G, Pan Y . Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage. Bioresour Technol. 2017; 238:706-715. DOI: 10.1016/j.biortech.2017.04.055. View

18.

Gharechahi J, Kharazian Z, Sarikhan S, Salehi Jouzani G, Aghdasi M, Hosseini Salekdeh G . The dynamics of the bacterial communities developed in maize silage. Microb Biotechnol. 2017; 10(6):1663-1676. PMC: 5658587. DOI: 10.1111/1751-7915.12751. View

19.

Ogunade I, Jiang Y, Pech Cervantes A, Kim D, Oliveira A, Vyas D . Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: Effects of Escherichia coli O157:H7 and silage additives. J Dairy Sci. 2017; 101(3):2048-2059. DOI: 10.3168/jds.2017-12876. View

20.

Keshri J, Chen Y, Pinto R, Kroupitski Y, Weinberg Z, Sela Saldinger S . Microbiome dynamics during ensiling of corn with and without Lactobacillus plantarum inoculant. Appl Microbiol Biotechnol. 2018; 102(9):4025-4037. DOI: 10.1007/s00253-018-8903-y. View