Study on the Material Basis of Houpo Wenzhong Decoction by HPLC Fingerprint, UHPLC-ESI-LTQ-Orbitrap-MS, and Network Pharmacology

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2019 Jul 25

PMID 31337148

Citations 4

Authors

Juyuan Luo

Gongsen Chen

Donghan Liu

Yan Wang

Qi Qi

Haiyan Hu

Pengyue Li

Jie Bai

Shouying Du

Yang Lu

Yuming Wang

Cun Liu

Affiliations

Soon will be listed here.

Abstract

Houpo Wenzhong Decoction (HWD) as a classical prescription has been widely used for hundreds of years. However, the quality of HWDs is difficult to control because of its herb materials from different regions. It is a new idea to use HPLC fingerprints, LTQ-ESI-Orbitrap-MS, and network pharmacology in combination to screen common components (CCs) as potential quality control indicators. In this paper, the fingerprints of HWDs were established, which were used to determine HWDs compounded from different sources of traditional Chinese medicines (TCMs). Through the similarity analysis, 45 CCs were selected. UHPLC-LTQ-ESI-Orbitrap-MS was used to carry out the chemical composition analysis of HWD. Seventy-three chemical constituents were distinguished, and 30 CCs were identified. Through network pharmacology, networks of candidate CCs, diseases, and candidate targets were constructed. Finally, four CCs were screened as potential active ingredient markers of HWD, and a method for content determination of these four components was established. A rapid, reasonable, and effective method for quality evaluation and control of HWDs was established. It provides a reference for the further development and research of HWDs and a new way of thinking for the research of other Chinese medicine prescriptions.

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References

Zhang C, Yang L, Yu X, Yu S, Yuan Y, Chen C . [Effects of tree ages and geographic area on quality of bark of Magnolia officinalis and M. officinalis var. biloba]. Zhongguo Zhong Yao Za Zhi. 2010; 34(19):2431-7. View

Yu S, Zhang C, Chen C, Yan R, Yang B, Liao C . [Effects of primary processing on quality of cortex Magnolia officinalis]. Zhongguo Zhong Yao Za Zhi. 2010; 35(14):1831-5. DOI: 10.4268/cjcmm20101414. View

Yang L, Ding J, Maxwell P, McCooeye M, Windust A, Ouerdane L . Determination of arsenobetaine in fish tissue by species specific isotope dilution LC-LTQ-Orbitrap-MS and standard addition LC-ICPMS. Anal Chem. 2011; 83(9):3371-8. DOI: 10.1021/ac103258m. View

Tao W, Xu X, Wang X, Li B, Wang Y, Li Y . Network pharmacology-based prediction of the active ingredients and potential targets of Chinese herbal Radix Curcumae formula for application to cardiovascular disease. J Ethnopharmacol. 2012; 145(1):1-10. DOI: 10.1016/j.jep.2012.09.051. View

Zhang Y, Tang F . [Advance in latest studies on pharmacological effects of magnolol]. Zhongguo Zhong Yao Za Zhi. 2013; 37(23):3526-30. View

Lin Z, Yang R, Guan Z, Chen A, Li W . Ultra-performance LC separation and quadrupole time-of-flight MS identification of major alkaloids in Plumula Nelumbinis. Phytochem Anal. 2014; 25(6):485-94. PMC: 5361407. DOI: 10.1002/pca.2517. View

Wang P, Wang B, Xu J, Sun J, Yan Q, Ji B . Detection and chemical profiling of Ling-Gui-Zhu-Gan decoction by ultra performance liquid chromatography-hybrid linear ion trap-Orbitrap mass spectrometry. J Chromatogr Sci. 2014; 53(2):263-73. DOI: 10.1093/chromsci/bmu051. View

Sun X, Wang H, Han X, Chen S, Zhu S, Dai J . Fingerprint analysis of polysaccharides from different Ganoderma by HPLC combined with chemometrics methods. Carbohydr Polym. 2014; 114:432-439. DOI: 10.1016/j.carbpol.2014.08.048. View

Tang F, Tang Q, Tian Y, Fan Q, Huang Y, Tan X . Network pharmacology-based prediction of the active ingredients and potential targets of Mahuang Fuzi Xixin decoction for application to allergic rhinitis. J Ethnopharmacol. 2015; 176:402-12. DOI: 10.1016/j.jep.2015.10.040. View

10.

Zou D, Wang J, Zhang B, Xie S, Wang Q, Xu K . Analysis of Chemical Constituents in Wuzi-Yanzong-Wan by UPLC-ESI-LTQ-Orbitrap-MS. Molecules. 2015; 20(12):21373-404. PMC: 6332400. DOI: 10.3390/molecules201219765. View

11.

Hou Y, Nie Y, Cheng B, Tao J, Ma X, Jiang M . Qingfei Xiaoyan Wan, a traditional Chinese medicine formula, ameliorates Pseudomonas aeruginosa-induced acute lung inflammation by regulation of PI3K/AKT and Ras/MAPK pathways. Acta Pharm Sin B. 2016; 6(3):212-21. PMC: 4856955. DOI: 10.1016/j.apsb.2016.03.002. View

12.

Peng G, Guan H, Wang X, Shi Y . Simultaneous determination of 14 active constituents of Shengjiang Xiexin decoction using ultrafast liquid chromatography coupled with electrospray ionization tandem mass spectrometry. Acta Pharm Sin B. 2017; 7(2):193-201. PMC: 5343114. DOI: 10.1016/j.apsb.2016.11.006. View

13.

Liu Z, Wang C, Zhu X, Wan J, Zhang J, Li W . Dynamic Changes in Neutral and Acidic Ginsenosides with Different Cultivation Ages and Harvest Seasons: Identification of Chemical Characteristics for Panax ginseng Quality Control. Molecules. 2017; 22(5). PMC: 6153749. DOI: 10.3390/molecules22050734. View

14.

Huang J, Cheung F, Tan H, Hong M, Wang N, Yang J . Identification of the active compounds and significant pathways of yinchenhao decoction based on network pharmacology. Mol Med Rep. 2017; 16(4):4583-4592. PMC: 5646998. DOI: 10.3892/mmr.2017.7149. View

15.

Sasot G, Martinez-Huelamo M, Vallverdu-Queralt A, Mercader-Marti M, Estruch R, Lamuela-Raventos R . Identification of phenolic metabolites in human urine after the intake of a functional food made from grape extract by a high resolution LTQ-Orbitrap-MS approach. Food Res Int. 2017; 100(Pt 3):435-444. DOI: 10.1016/j.foodres.2017.01.020. View

16.

Zeng L, Yang K, Ge J . Uncovering the Pharmacological Mechanism of Astragalus Salvia Compound on Pregnancy-Induced Hypertension Syndrome by a Network Pharmacology Approach. Sci Rep. 2017; 7(1):16849. PMC: 5715142. DOI: 10.1038/s41598-017-17139-x. View

17.

Gu J, Wu W, Huang M, Long F, Liu X, Zhu Y . Application of High-Performance Liquid Chromatography Coupled with Linear Ion Trap Quadrupole Orbitrap Mass Spectrometry for Qualitative and Quantitative Assessment of Shejin-Liyan Granule Supplements. Molecules. 2018; 23(4). PMC: 6017834. DOI: 10.3390/molecules23040884. View

18.

Zhang X, Zhao R, Chen M, Ma T, Wu G, Xue N . Novel Neuroprotective Lead Compound Ligustrazine Derivative Mass Spectrometry Fragmentation Rule and Metabolites in Rats by LC/LTQ-Orbitrap MS. Molecules. 2018; 23(5). PMC: 6100194. DOI: 10.3390/molecules23051154. View

19.

Hu M, Bai M, Ye W, Wang Y, Wu H . Variations in Volatile Oil Yield and Composition of "Xin-yi" (Magnolia biondii Pamp. Flower Buds) at Different Growth Stages. J Oleo Sci. 2018; 67(6):779-787. DOI: 10.5650/jos.ess17229. View

20.

Zhang J, Xu W, Wang P, Huang J, Bai J, Huang Z . Chemical Analysis and Multi-Component Determination in Chinese Medicine Preparation Bupi Yishen Formula Using Ultra-High Performance Liquid Chromatography With Linear Ion Trap-Orbitrap Mass Spectrometry and Triple-Quadrupole Tandem Mass Spectrometry. Front Pharmacol. 2018; 9:568. PMC: 6002530. DOI: 10.3389/fphar.2018.00568. View