» Articles » PMID: 31333723

Analysis of Long Non-Coding RNA and MRNA Expression Profiling in Immature and Mature Bovine () Testes

Overview
Journal Front Genet
Date 2019 Jul 24
PMID 31333723
Citations 38
Authors
Affiliations
Soon will be listed here.
Abstract

Testis development and spermatogenesis are strictly regulated by numbers of genes and non-coding genes. However, long non-coding RNAs (lncRNAs) as key regulators in multitudinous biological processes have not been systematically identified in bovine testes during sexual maturation. In this study, we comprehensively analyzed lncRNA and mRNA expression profiling of six bovine testes at 3 days after birth and 13 months by RNA sequencing. 23,735 lncRNAs and 22,118 mRNAs were identified, in which 540 lncRNAs (-value < 0.05) and 3,525 mRNAs (-adjust < 0.05) were significantly differentially expressed (DE) between two stages. Correspondingly, the results of RT-qPCR analysis showed well correlation with the transcriptome data. Moreover, GO and KEGG enrichment analyses showed that DE genes and target genes of DE lncRNAs were enriched in spermatogenesis. Furthermore, we constructed lncRNA-gene interaction networks; consequently, 15 DE lncRNAs and 12 -target genes were involved. The target genes (, , , , , , , and ) were found associated with bovine sexual maturation. In addition, the expression of lncRNAs and -target genes was detected in bovine Leydig cells, Sertoli cells, and spermatogonia. Our study identified and analyzed lncRNAs and mRNAs in testis tissues, suggesting that lncRNAs may regulate testis development and spermatogenesis. Our findings provided new insights for further investigation of biological function in bovine lncRNA.

Citing Articles

Comprehensive analysis of long non-coding RNA and mRNA expression patterns during seminiferous tubules maturation in Guanzhong dairy goats.

Ren F, Zhang Q, Jiang Y, Xie W, Qiao P, Hu J BMC Genomics. 2025; 26(1):159.

PMID: 39966702 PMC: 11834200. DOI: 10.1186/s12864-025-11320-7.


Evolutionary innovations in germline biology of placental mammals identified by transcriptomics of first-wave spermatogenesis in opossum.

Marshall K, Stadtmauer D, Maziarz J, Wagner G, Lesch B Dev Cell. 2024; 60(4):646-664.e8.

PMID: 39536760 PMC: 11859772. DOI: 10.1016/j.devcel.2024.10.013.


Identification of Heilongjiang crossbred beef cattle pedigrees and reveals functional genes related to economic traits based on whole-genome SNP data.

Li S, Liu L, Ahmed Z, Wang F, Lei C, Sun F Front Genet. 2024; 15:1435793.

PMID: 39119576 PMC: 11306169. DOI: 10.3389/fgene.2024.1435793.


Sperm long non-coding RNAs as markers for ram fertility.

Hitit M, Kaya A, Memili E Front Vet Sci. 2024; 11:1337939.

PMID: 38799722 PMC: 11117017. DOI: 10.3389/fvets.2024.1337939.


Risk of Sperm Disorders and Impaired Fertility in Frozen-Thawed Bull Semen: A Genome-Wide Association Study.

Dementieva N, Dysin A, Shcherbakov Y, Nikitkina E, Musidray A, Petrova A Animals (Basel). 2024; 14(2).

PMID: 38254422 PMC: 10812825. DOI: 10.3390/ani14020251.


References
1.
Fujita A, Nakamura K, Kato T, Watanabe N, Ishizaki T, Kimura K . Ropporin, a sperm-specific binding protein of rhophilin, that is localized in the fibrous sheath of sperm flagella. J Cell Sci. 1999; 113 ( Pt 1):103-12. DOI: 10.1242/jcs.113.1.103. View

2.
Moreira E, Jaworski C, Li A, Rodriguez I . Molecular and biochemical characterization of a novel oxysterol-binding protein (OSBP2) highly expressed in retina. J Biol Chem. 2001; 276(21):18570-8. DOI: 10.1074/jbc.M011259200. View

3.
Bateman A, Birney E, Cerruti L, Durbin R, Etwiller L, Eddy S . The Pfam protein families database. Nucleic Acids Res. 2001; 30(1):276-80. PMC: 99071. DOI: 10.1093/nar/30.1.276. View

4.
Sleutels F, Zwart R, Barlow D . The non-coding Air RNA is required for silencing autosomal imprinted genes. Nature. 2002; 415(6873):810-3. DOI: 10.1038/415810a. View

5.
Xu M, Xiao J, Chen J, Li J, Yin L, Zhu H . Identification and characterization of a novel human testis-specific Golgi protein, NYD-SP12. Mol Hum Reprod. 2003; 9(1):9-17. DOI: 10.1093/molehr/gag005. View