C-myc Expression in Lymphocytes of MRL/MP-lpr Mice Activated by A23187 and TPA

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1988 May 1

PMID 3133310

Authors

T Koizumi

Y Nakao

T Matsui

Y Katakami

K Mihara

R Takahashi

S Maeda

T Sugiyama

T Fujita

Affiliations

Soon will be listed here.

Abstract

In order to analyse the molecular mechanism of lymphocyte activation defect in MRL/MP-lpr (MRL/1) mice, c-myc proto-oncogene expression was examined in MRL/1 lymph node cells stimulated by various agents. Since a transient increase of c-myc RNA in early hours is required for lymphocyte activation, detection of c-myc messenger RNA is useful to determine whether or not an appropriate signal is transduced to the nucleus. Stimulation by concanavalin A (Con A) plus 12-O-tetradecanoyl phorbol 13-acetate (TPA) or A23187 plus TPA markedly increased c-myc mRNA expression and cell proliferation, whereas stimulation by Con A alone failed to do so. These results suggest that an abnormality exists in the early signal transduction process, and that it could be bypassed by calcium influx and direct activation of protein kinase C.

References

Andrews B, Eisenberg R, Theofilopoulos A, Izui S, Wilson C, McCONAHEY P . Spontaneous murine lupus-like syndromes. Clinical and immunopathological manifestations in several strains. J Exp Med. 1978; 148(5):1198-215. PMC: 2185049. DOI: 10.1084/jem.148.5.1198. View

Santoro T, Malek T, Rosenberg Y, Morse 3rd H, Steinberg A . Signals required for activation and growth of autoimmune T lymphocytes. J Mol Cell Immunol. 1984; 1(6):347-56. View

Altman A, Theofilopoulos A, Weiner R, Katz D, Dixon F . Analysis of T cell function in autoimmune murine strains. Defects in production and responsiveness to interleukin 2. J Exp Med. 1981; 154(3):791-808. PMC: 2186441. DOI: 10.1084/jem.154.3.791. View

Wofsy D, MURPHY E, Roths J, Dauphinee M, Kipper S, Talal N . Deficient interleukin 2 activity in MRL/Mp and C57BL/6J mice bearing the lpr gene. J Exp Med. 1981; 154(5):1671-80. PMC: 2186500. DOI: 10.1084/jem.154.5.1671. View

Dumont F, Habbersett R . Unusual cell surface properties of the T lymphocyte population expanding in MRL/Mp-lpr/lpr mice. Immunology. 1982; 47(2):271-81. PMC: 1555445. View

Morse 3rd H, Davidson W, Yetter R, MURPHY E, Roths J, Coffman R . Abnormalities induced by the mutant gene Ipr: expansion of a unique lymphocyte subset. J Immunol. 1982; 129(6):2612-5. View

TAUB R, Kirsch I, Morton C, Lenoir G, Swan D, Tronick S . Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc Natl Acad Sci U S A. 1982; 79(24):7837-41. PMC: 347444. DOI: 10.1073/pnas.79.24.7837. View

Kelly K, Cochran B, Stiles C, Leder P . Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor. Cell. 1983; 35(3 Pt 2):603-10. DOI: 10.1016/0092-8674(83)90092-2. View

NISHIZUKA Y . The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature. 1984; 308(5961):693-8. DOI: 10.1038/308693a0. View

10.

Wofsy D, Hardy R, Seaman W . The proliferating cells in autoimmune MRL/lpr mice lack L3T4, an antigen on "helper" T cells that is involved in the response to class II major histocompatibility antigens. J Immunol. 1984; 132(6):2686-9. View

11.

Cantrell D, Smith K . The interleukin-2 T-cell system: a new cell growth model. Science. 1984; 224(4655):1312-6. DOI: 10.1126/science.6427923. View

12.

Truneh A, Albert F, Golstein P . Early steps of lymphocyte activation bypassed by synergy between calcium ionophores and phorbol ester. Nature. 1985; 313(6000):318-20. DOI: 10.1038/313318a0. View

13.

Smeland E, Godal T, Ruud E, Beiske K, Funderud S, Clark E . The specific induction of myc protooncogene expression in normal human B cells is not a sufficient event for acquisition of competence to proliferate. Proc Natl Acad Sci U S A. 1985; 82(18):6255-9. PMC: 391031. DOI: 10.1073/pnas.82.18.6255. View

14.

Granelli-Piperno A, Andrus L, Steinman R . Lymphokine and nonlymphokine mRNA levels in stimulated human T cells. Kinetics, mitogen requirements, and effects of cyclosporin A. J Exp Med. 1986; 163(4):922-37. PMC: 2188080. DOI: 10.1084/jem.163.4.922. View

15.

Schneider-Schaulies J, Hunig T, Schimpl A, WECKER E . Kinetics of cellular oncogene expression in mouse lymphocytes. I. Expression of c-myc and c-ras-Ha in T lymphocytes induced by various mitogens. Eur J Immunol. 1986; 16(3):312-6. DOI: 10.1002/eji.1830160319. View

16.

Klinman D, MUSHINSKI J, Honda M, Ishigatsubo Y, Mountz J, Raveche E . Oncogene expression in autoimmune and normal peripheral blood mononuclear cells. J Exp Med. 1986; 163(5):1292-307. PMC: 2188108. DOI: 10.1084/jem.163.5.1292. View

17.

Matsui T, Takahashi R, Nakao Y, Koizumi T, Katakami Y, Mihara K . 1,25-Dihydroxyvitamin D3-regulated expression of genes involved in human T-lymphocyte proliferation and differentiation. Cancer Res. 1986; 46(11):5827-31. View

18.

Katagiri K, Katagiri T, Eisenberg R, Ting J, Cohen P . Interleukin 2 responses of lpr and normal L3T4-/Lyt-2- T cells induced by TPA plus A23187. J Immunol. 1987; 138(1):149-56. View

19.

Miescher G, Budd R, Lees R, MacDonald H . Abnormal expression of T cell receptor genes in Lyt-2- L3T4- lymphocytes of lpr mice: comparison with normal immature thymocytes. J Immunol. 1987; 138(6):1959-67. View

20.

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View