Potential Transmission Sources of Infection: Detection of in Various Environmental Samples

Overview

Journal J Environ Health Sci Eng

Publisher Springer

Date 2019 Jul 20

PMID 31321040

Citations 8

Authors

Marzieh Farhadkhani

Mahnaz Nikaeen

Akbar Hassanzadeh

Bahram Nikmanesh

Affiliations

Soon will be listed here.

Abstract

Purpose: is one of the most common human infectious agents which may be transmitted via water. This study was designed to test presence via molecular methods in various aquatic environments as well as sewage sludge (SS) to understand the role of these environments in the pathogen's transmission.

Methods: specific primers for the 16S rRNA and A genes of were used in a nested and semi-nested PCR, respectively. Detection sensitivity of in environmental samples by semi-nested PCR was also compared with real-time PCR. Analysis of fecal coliforms (FC) as pollution indicator bacteria was also performed.

Results: 16S rRNA gene was detected in 36% (14/39) of wastewater samples and 8% (2/25) of water samples, while amplification of A gene yielded only two positive result. None of the SS samples were positive for and real-time PCR could not identify in any of the samples. The results showed no correlation between the presence of and FC.

Conclusions: Our result revealed the widespread presence of in wastewater samples which indicates wastewater may be a source for dissemination and transmission of infection. Further research is needed to determine the risk of in wastewater reuse for irrigation of crops.

Citing Articles

Prevalence of infection in China from 2014-2023: A systematic review and meta-analysis.

Xie L, Liu G, Liu Y, Li P, Hu X, He X World J Gastroenterol. 2024; 30(43):4636-4656.

PMID: 39575409 PMC: 11572641. DOI: 10.3748/wjg.v30.i43.4636.

Towards geospatially-resolved public-health surveillance via wastewater sequencing.

Tierney B, Foox J, Ryon K, Butler D, Damle N, Young B Nat Commun. 2024; 15(1):8386.

PMID: 39333485 PMC: 11436780. DOI: 10.1038/s41467-024-52427-x.

Rapid profiling of carcinogenic types of infection via deep learning analysis of label-free SERS spectra of human serum.

Li F, Si Y, Tang J, Umar Z, Xiong X, Wang J Comput Struct Biotechnol J. 2024; 23:3379-3390.

PMID: 39329094 PMC: 11424770. DOI: 10.1016/j.csbj.2024.09.008.

Intracellular presence of Helicobacter pylori antigen and genes within gastric and vaginal Candida.

Yang T, Li J, Zhang Y, Deng Z, Cui G, Yuan J PLoS One. 2024; 19(2):e0298442.

PMID: 38329956 PMC: 10852334. DOI: 10.1371/journal.pone.0298442.

Molecular characterization of Helicobacter pylori isolated from Nile Tilapia (Oreochromis niloticus) and fish handlers.

Mubarak A, Abd-Elhafeez H, Mohamed H BMC Vet Res. 2023; 19(1):250.

PMID: 38031127 PMC: 10685712. DOI: 10.1186/s12917-023-03819-6.

References

Baker K, Hegarty J . Presence of Helicobacter pylori in drinking water is associated with clinical infection. Scand J Infect Dis. 2001; 33(10):744-6. DOI: 10.1080/003655401317074536. View

Moreno Y, Ferrus M, Alonso J, Jimenez A, Hernandez J . Use of fluorescent in situ hybridization to evidence the presence of Helicobacter pylori in water. Water Res. 2003; 37(9):2251-6. DOI: 10.1016/S0043-1354(02)00624-3. View

Karita M, Teramukai S, Matsumoto S . Risk of Helicobacter pylori transmission from drinking well water is higher than that from infected intrafamilial members in Japan. Dig Dis Sci. 2003; 48(6):1062-7. DOI: 10.1023/a:1023752326137. View

Jeggli S, Steiner D, Joller H, Tschopp A, Steffen R, Hotz P . Hepatitis E, Helicobacter pylori, and gastrointestinal symptoms in workers exposed to waste water. Occup Environ Med. 2004; 61(7):622-7. PMC: 1740808. DOI: 10.1136/oem.2003.011411. View

Smith S, Oyedeji K, Arigbabu A, Cantet F, Megraud F, Ojo O . Comparison of three PCR methods for detection of Helicobacter pylori DNA and detection of cagA gene in gastric biopsy specimens. World J Gastroenterol. 2004; 10(13):1958-60. PMC: 4572239. DOI: 10.3748/wjg.v10.i13.1958. View

Rolle-Kampczyk U, Fritz G, Diez U, Lehmann I, Richter M, Herbarth O . Well water--one source of Helicobacter pylori colonization. Int J Hyg Environ Health. 2004; 207(4):363-8. DOI: 10.1078/1438-4639-00301. View

Rinttila T, Kassinen A, Malinen E, Krogius L, Palva A . Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol. 2004; 97(6):1166-77. DOI: 10.1111/j.1365-2672.2004.02409.x. View

Queralt N, Bartolome R, Araujo R . Detection of Helicobacter pylori DNA in human faeces and water with different levels of faecal pollution in the north-east of Spain. J Appl Microbiol. 2005; 98(4):889-95. DOI: 10.1111/j.1365-2672.2004.02523.x. View

McDaniels A, Wymer L, Rankin C, Haugland R . Evaluation of quantitative real time PCR for the measurement of Helicobacter pylori at low concentrations in drinking water. Water Res. 2005; 39(19):4808-16. DOI: 10.1016/j.watres.2005.09.030. View

10.

Kusters J, Van Vliet A, Kuipers E . Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev. 2006; 19(3):449-90. PMC: 1539101. DOI: 10.1128/CMR.00054-05. View

11.

Moreno Y, Piqueres P, Alonso J, Jimenez A, Gonzalez A, Ferrus M . Survival and viability of Helicobacter pylori after inoculation into chlorinated drinking water. Water Res. 2007; 41(15):3490-6. DOI: 10.1016/j.watres.2007.05.020. View

12.

Sugimoto M, Wu J, Abudayyeh S, Hoffman J, Brahem H, Al-Khatib K . Unreliability of results of PCR detection of Helicobacter pylori in clinical or environmental samples. J Clin Microbiol. 2009; 47(3):738-42. PMC: 2650922. DOI: 10.1128/JCM.01563-08. View

13.

Janzon A, Sjoling A, Lothigius A, Ahmed D, Qadri F, Svennerholm A . Failure to detect Helicobacter pylori DNA in drinking and environmental water in Dhaka, Bangladesh, using highly sensitive real-time PCR assays. Appl Environ Microbiol. 2009; 75(10):3039-44. PMC: 2681616. DOI: 10.1128/AEM.02779-08. View

14.

Van Hooste W, Charlier A, Rotsaert P, Bulterys S, Moens G, Van Sprundel M . Work-related Helicobacter pylori infection among sewage workers in municipal wastewater treatment plants in Belgium. Occup Environ Med. 2010; 67(2):91-7. DOI: 10.1136/oem.2008.040436. View

15.

Khalifa M, Sharaf R, Aziz R . Helicobacter pylori: a poor man's gut pathogen?. Gut Pathog. 2010; 2(1):2. PMC: 2861632. DOI: 10.1186/1757-4749-2-2. View

16.

Twing K, Kirchman D, Campbell B . Temporal study of Helicobacter pylori presence in coastal freshwater, estuary and marine waters. Water Res. 2011; 45(4):1897-905. DOI: 10.1016/j.watres.2010.12.013. View

17.

Moreno Y, Ferrus M . Specific detection of cultivable Helicobacter pylori cells from wastewater treatment plants. Helicobacter. 2012; 17(5):327-32. DOI: 10.1111/j.1523-5378.2012.00961.x. View

18.

Asghari F, Nikaeen M, Mirhendi H . Rapid monitoring of Pseudomonas aeruginosa in hospital water systems: a key priority in prevention of nosocomial infection. FEMS Microbiol Lett. 2013; 343(1):77-81. DOI: 10.1111/1574-6968.12132. View

19.

Holman C, Bachoon D, Otero E, Ramsubhag A . Detection of Helicobacter pylori in the coastal waters of Georgia, Puerto Rico and Trinidad. Mar Pollut Bull. 2013; 79(1-2):354-8. DOI: 10.1016/j.marpolbul.2013.11.021. View

20.

Nikaeen M, Aghili Dehnavi H, Hssanzadeh A, Jalali M . Occurrence of Clostridium difficile in two types of wastewater treatment plants. J Formos Med Assoc. 2015; 114(7):663-5. DOI: 10.1016/j.jfma.2014.12.005. View