Evaluation of Mechanical Properties and Cell Viability of Poly (3-Hydroxybutyrate)-Chitosan/AlO Nanocomposite Scaffold for Cartilage Tissue Engineering

Overview

Journal J Med Signals Sens

Specialty Biomedical Engineering

Date 2019 Jul 19

PMID 31316904

Citations 5

Authors

Elahe Bahremandi Toloue

Saeed Karbasi

Hossein Salehi

Mohammad Rafienia

Affiliations

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Abstract

Background: The aim of this study was to evaluate the effects of alumina nanowires as reinforcement phases in polyhydroxybutyrate-chitosan (PHB-CTS) scaffolds to apply in cartilage tissue engineering.

Methods: A certain proportion of polymers and alumina was chosen. After optimization of electrospun parameters, PHB, PHB-CTS, and PHB-CTS/3% AlO nanocomposite scaffolds were randomly electrospun. Scanning electron microscopy, Fourier transform infrared spectroscopy, water contact angle measurement, tensile strength, and chondrocyte cell culture studies were used to evaluate the physical, mechanical, and biological properties of the scaffolds.

Results: The average fiber diameter of scaffolds was 300-550 nm and the porosity percentages for the first layer of all types of scaffolds were more than 81%. Scaffolds' hydrophilicity was increased by adding alumina and CTS. The tensile strength of scaffolds decreased by adding CTS and increased up to more than 10 folds after adding alumina. Chondrocyte viability and proliferation on scaffolds were better after adding CTS and alumina to PHB.

Conclusion: With regard to the results, electrospun PHB-CTS/3% AlO scaffold has the appropriate potential to apply in cartilage tissue engineering.

Citing Articles

Microbial Polyhydroxyalkanoates Granules: An Approach Targeting Biopolymer for Medical Applications and Developing Bone Scaffolds.

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Fabrication of Silk-Hyaluronan Composite as a Potential Scaffold for Tissue Repair.

Yu L, Liu T, Ma Y, Zhang F, Huang Y, Fan Z Front Bioeng Biotechnol. 2020; 8:578988.

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Comprehensive Survey on Nanobiomaterials for Bone Tissue Engineering Applications.

Kumar P, Saini M, Dehiya B, Sindhu A, Kumar V, Kumar R Nanomaterials (Basel). 2020; 10(10).

PMID: 33066127 PMC: 7601994. DOI: 10.3390/nano10102019.

Electrospun Polymers in Cartilage Engineering-State of Play.

Yilmaz E, Zeugolis D Front Bioeng Biotechnol. 2020; 8:77.

PMID: 32133352 PMC: 7039817. DOI: 10.3389/fbioe.2020.00077.

Recent Advances in the Use of Polyhydroyalkanoates in Biomedicine.

Rodriguez-Contreras A Bioengineering (Basel). 2019; 6(3).

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References

Thambyah A, Nather A, Goh J . Mechanical properties of articular cartilage covered by the meniscus. Osteoarthritis Cartilage. 2006; 14(6):580-8. DOI: 10.1016/j.joca.2006.01.015. View

Roseti L, Parisi V, Petretta M, Cavallo C, Desando G, Bartolotti I . Scaffolds for Bone Tissue Engineering: State of the art and new perspectives. Mater Sci Eng C Mater Biol Appl. 2017; 78:1246-1262. DOI: 10.1016/j.msec.2017.05.017. View

Rodriguez-Lorenzo L, Salinas A, Vallet-Regi M, San Roman J . Composite biomaterials based on ceramic polymers. I. Reinforced systems based on Al2O3/PMMA/PLLA. J Biomed Mater Res. 1996; 30(4):515-22. DOI: 10.1002/(SICI)1097-4636(199604)30:4<515::AID-JBM10>3.0.CO;2-G. View

Song Y, Ju Y, Song G, Morita Y . In vitro proliferation and osteogenic differentiation of mesenchymal stem cells on nanoporous alumina. Int J Nanomedicine. 2013; 8:2745-56. PMC: 3735283. DOI: 10.2147/IJN.S44885. View

Asran A, Razghandi K, Aggarwal N, Michler G, Groth T . Nanofibers from blends of polyvinyl alcohol and polyhydroxy butyrate as potential scaffold material for tissue engineering of skin. Biomacromolecules. 2010; 11(12):3413-21. DOI: 10.1021/bm100912v. View

Khoroushi M, Foroughi M, Karbasi S, Hashemibeni B, Khademi A . Effect of Polyhydroxybutyrate/Chitosan/Bioglass nanofiber scaffold on proliferation and differentiation of stem cells from human exfoliated deciduous teeth into odontoblast-like cells. Mater Sci Eng C Mater Biol Appl. 2018; 89:128-139. DOI: 10.1016/j.msec.2018.03.028. View

Patel A, Balani K . Dispersion fraction enhances cellular growth of carbon nanotube and aluminum oxide reinforced ultrahigh molecular weight polyethylene biocomposites. Mater Sci Eng C Mater Biol Appl. 2014; 46:504-13. DOI: 10.1016/j.msec.2014.10.075. View

Hutmacher D . Scaffolds in tissue engineering bone and cartilage. Biomaterials. 2000; 21(24):2529-43. DOI: 10.1016/s0142-9612(00)00121-6. View

Nagiah N, Madhavi L, Anitha R, Anandan C, Srinivasan N, Tirichurapalli Sivagnanam U . Development and characterization of coaxially electrospun gelatin coated poly (3-hydroxybutyric acid) thin films as potential scaffolds for skin regeneration. Mater Sci Eng C Mater Biol Appl. 2013; 33(7):4444-52. DOI: 10.1016/j.msec.2013.06.042. View

10.

Dong Z, Wu Y, Wang Q, Xie C, Ren Y, Clark R . Reinforcement of electrospun membranes using nanoscale Al2O3 whiskers for improved tissue scaffolds. J Biomed Mater Res A. 2012; 100(4):903-10. DOI: 10.1002/jbm.a.34027. View

11.

Zhijiang C, Yi X, Haizheng Y, Jia J, Liu Y . Poly(hydroxybutyrate)/cellulose acetate blend nanofiber scaffolds: Preparation, characterization and cytocompatibility. Mater Sci Eng C Mater Biol Appl. 2015; 58:757-67. DOI: 10.1016/j.msec.2015.09.048. View

12.

Medvecky L, Giretova M, Stulajterova R . Properties and in vitro characterization of polyhydroxybutyrate-chitosan scaffolds prepared by modified precipitation method. J Mater Sci Mater Med. 2013; 25(3):777-89. DOI: 10.1007/s10856-013-5105-0. View

13.

Hashimoto M, Sasaki J, Imazato S . Investigation of the cytotoxicity of aluminum oxide nanoparticles and nanowires and their localization in L929 fibroblasts and RAW264 macrophages. J Biomed Mater Res B Appl Biomater. 2015; 104(2):241-52. DOI: 10.1002/jbm.b.33377. View

14.

Karlsson M, Palsgard E, Wilshaw P, Di Silvio L . Initial in vitro interaction of osteoblasts with nano-porous alumina. Biomaterials. 2003; 24(18):3039-46. DOI: 10.1016/s0142-9612(03)00146-7. View