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Reverse ELISPOT Assay for Clonal Analysis of Cytokine Production. I. Enumeration of Gamma-interferon-secreting Cells

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Publisher Elsevier
Date 1988 May 25
PMID 3131436
Citations 106
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Abstract

A reverse modification of ELISPOT assay using nitrocellulose membranes and epitope-specific monoclonal antibodies is described for the detection of single lymphokine-secreting cells. As a model, the production of gamma-interferon by mitogen stimulated human peripheral blood lymphocytes has been examined. The assay can also be modified to permit microscopic examination of spot-forming cells.

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