Comparison of Two Commercial Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) Systems for Identification of Nontuberculous Mycobacteria

Overview

Journal Am J Clin Pathol

Publisher Oxford University Press

Specialty Pathology

Date 2019 Jul 18

PMID 31314059

Citations 13

Authors

Barbara A Brown-Elliott

Thomas R Fritsche

Brooke J Olson

Sruthi Vasireddy

Ravikiran Vasireddy

Elena Iakhiaeva

Diana Alame

Richard J Wallace

John A Branda

Affiliations

Soon will be listed here.

Abstract

Objectives: This multicenter study's aim was to assess the performance of two commercially available matrix-assisted laser desorption/ionization time of flight mass spectrometry systems in identifying a challenge collection of clinically relevant nontuberculous mycobacteria (NTM).

Methods: NTM clinical isolates (n = 244) belonging to 23 species/subspecies were identified by gene sequencing and analyzed using Bruker Biotyper with Mycobacterial Library v5.0.0 and bioMérieux VITEK MS with v3.0 database.

Results: Using the Bruker or bioMérieux systems, 92% and 95% of NTM strains, respectively, were identified at least to the complex/group level; 62% and 57%, respectively, were identified to the highest taxonomic level. Differentiation between members of Mycobacterium abscessus, M fortuitum, M mucogenicum, M avium, and M terrae complexes/groups was problematic for both systems, as was identification of M chelonae for the Bruker system.

Conclusions: Both systems identified most NTM isolates to the group/complex level, and many to the highest taxonomic level. Performance was comparable.

Citing Articles

Precise mycobacterial species and subspecies identification using the PEP-TORCH peptidome algorithm.

Bao D, Maity S, Zhan L, Seo S, Shu Q, Lyon C EMBO Mol Med. 2025; .

PMID: 40038396 DOI: 10.1038/s44321-025-00207-5.

Diagnostic performance of an automated robot for MALDI target preparation in microbial identification.

Pranada A, Cicatka M, Hess C, Karasek J J Clin Microbiol. 2024; 62(10):e0043424.

PMID: 39297624 PMC: 11481498. DOI: 10.1128/jcm.00434-24.

Contribution of machine learning for subspecies identification from Mycobacterium abscessus with MALDI-TOF MS in solid and liquid media.

Godmer A, Bigey L, Giai-Gianetto Q, Pierrat G, Mohammad N, Mougari F Microb Biotechnol. 2024; 17(9):e14545.

PMID: 39257027 PMC: 11387462. DOI: 10.1111/1751-7915.14545.

Emergence of Inducible Macrolide Resistance in Mycobacterium chelonae Due to Broad-Host-Range Plasmid and Chromosomal Variants of the Novel 23S rRNA Methylase Gene, (55).

Brown-Elliott B, Wallace Jr R, Wengenack N, Workman S, Cameron A, Bush G J Clin Microbiol. 2023; 61(7):e0042823.

PMID: 37347171 PMC: 10358161. DOI: 10.1128/jcm.00428-23.

Application of MALDI-TOF MS for enumerating bacterial constituents of defined consortia.

Coryell M, Sava R, Hastie J, Carlson Jr P Appl Microbiol Biotechnol. 2023; 107(12):4069-4077.

PMID: 37148337 PMC: 10238304. DOI: 10.1007/s00253-023-12558-5.

References

Brown-Elliott B, Wallace Jr R, Crist C, Mann L, Wilson R . Comparison of in vitro activities of gatifloxacin and ciprofloxacin against four taxa of rapidly growing mycobacteria. Antimicrob Agents Chemother. 2002; 46(10):3283-5. PMC: 128789. DOI: 10.1128/AAC.46.10.3283-3285.2002. View

Adekambi T, Colson P, Drancourt M . rpoB-based identification of nonpigmented and late-pigmenting rapidly growing mycobacteria. J Clin Microbiol. 2003; 41(12):5699-708. PMC: 308974. DOI: 10.1128/JCM.41.12.5699-5708.2003. View

Lefmann M, Honisch C, Bocker S, Storm N, von Wintzingerode F, Schlotelburg C . Novel mass spectrometry-based tool for genotypic identification of mycobacteria. J Clin Microbiol. 2004; 42(1):339-46. PMC: 321663. DOI: 10.1128/JCM.42.1.339-346.2004. View

Hettick J, Kashon M, Simpson J, Siegel P, Mazurek G, Weissman D . Proteomic profiling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Chem. 2004; 76(19):5769-76. DOI: 10.1021/ac049410m. View

Wallace Jr R, Brown-Elliott B, Brown J, Steigerwalt A, Hall L, Woods G . Polyphasic characterization reveals that the human pathogen Mycobacterium peregrinum type II belongs to the bovine pathogen species Mycobacterium senegalense. J Clin Microbiol. 2005; 43(12):5925-35. PMC: 1317161. DOI: 10.1128/JCM.43.12.5925-5935.2005. View

Pignone M, Greth K, Cooper J, Emerson D, Tang J . Identification of mycobacteria by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry. J Clin Microbiol. 2006; 44(6):1963-70. PMC: 1489414. DOI: 10.1128/JCM.01959-05. View

Nash K, Brown-Elliott B, Wallace Jr R . A novel gene, erm(41), confers inducible macrolide resistance to clinical isolates of Mycobacterium abscessus but is absent from Mycobacterium chelonae. Antimicrob Agents Chemother. 2009; 53(4):1367-76. PMC: 2663066. DOI: 10.1128/AAC.01275-08. View

Seng P, Drancourt M, Gouriet F, La Scola B, Fournier P, Rolain J . Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis. 2009; 49(4):543-51. DOI: 10.1086/600885. View

Bizzini A, Greub G . Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin Microbiol Infect. 2010; 16(11):1614-9. DOI: 10.1111/j.1469-0691.2010.03311.x. View

10.

Greub G . MALDI-TOF mass spectrometry: the quantum leap. Clin Microbiol Infect. 2010; 16(11):1603. DOI: 10.1111/j.1469-0691.2010.03380.x. View

11.

Lotz A, Ferroni A, Beretti J, Dauphin B, Carbonnelle E, Guet-Revillet H . Rapid identification of mycobacterial whole cells in solid and liquid culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2010; 48(12):4481-6. PMC: 3008439. DOI: 10.1128/JCM.01397-10. View

12.

Emonet S, Shah H, Cherkaoui A, Schrenzel J . Application and use of various mass spectrometry methods in clinical microbiology. Clin Microbiol Infect. 2010; 16(11):1604-13. DOI: 10.1111/j.1469-0691.2010.03368.x. View

13.

Saffert R, Cunningham S, Ihde S, Jobe K, Mandrekar J, Patel R . Comparison of Bruker Biotyper matrix-assisted laser desorption ionization-time of flight mass spectrometer to BD Phoenix automated microbiology system for identification of gram-negative bacilli. J Clin Microbiol. 2011; 49(3):887-92. PMC: 3067728. DOI: 10.1128/JCM.01890-10. View

14.

Saleeb P, Drake S, Murray P, Zelazny A . Identification of mycobacteria in solid-culture media by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol. 2011; 49(5):1790-4. PMC: 3122647. DOI: 10.1128/JCM.02135-10. View

15.

Brown-Elliott B, Nash K, Wallace Jr R . Antimicrobial susceptibility testing, drug resistance mechanisms, and therapy of infections with nontuberculous mycobacteria. Clin Microbiol Rev. 2012; 25(3):545-82. PMC: 3416486. DOI: 10.1128/CMR.05030-11. View

16.

Wallace Jr R, Bedsole G, Sumter G, Sanders C, Steele L, Brown B . Activities of ciprofloxacin and ofloxacin against rapidly growing mycobacteria with demonstration of acquired resistance following single-drug therapy. Antimicrob Agents Chemother. 1990; 34(1):65-70. PMC: 171521. DOI: 10.1128/AAC.34.1.65. View

17.

Rychert J, Burnham C, Bythrow M, Garner O, Ginocchio C, Jennemann R . Multicenter evaluation of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for identification of Gram-positive aerobic bacteria. J Clin Microbiol. 2013; 51(7):2225-31. PMC: 3697712. DOI: 10.1128/JCM.00682-13. View

18.

Chen J, Yam W, Ngan A, Fung A, Woo W, Yan M . Advantages of using matrix-assisted laser desorption ionization-time of flight mass spectrometry as a rapid diagnostic tool for identification of yeasts and mycobacteria in the clinical microbiological laboratory. J Clin Microbiol. 2013; 51(12):3981-7. PMC: 3838092. DOI: 10.1128/JCM.01437-13. View

19.

Mather C, Rivera S, Butler-Wu S . Comparison of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of mycobacteria using simplified protein extraction protocols. J Clin Microbiol. 2013; 52(1):130-8. PMC: 3911429. DOI: 10.1128/JCM.01996-13. View

20.

Tseng S, Teng S, Lee P, Wang C, Yu J, Lu P . Rapid identification of M. abscessus and M. massiliense by MALDI-TOF mass spectrometry with a comparison to sequencing methods and antimicrobial susceptibility patterns. Future Microbiol. 2013; 8(11):1381-9. DOI: 10.2217/fmb.13.115. View