Identifying a Novel Powdery Mildew Resistance Gene in a Barley Landrace from Morocco

Overview

Journal J Appl Genet

Publisher Springer

Specialty Genetics

Date 2019 Jul 18

PMID 31313063

Citations 8

Authors

Urszula Piechota

Pawel C Czembor

Piotr Slowacki

Jerzy H Czembor

Affiliations

Soon will be listed here.

Abstract

Powdery mildew is a barley foliar disease that causes great loss in yield. Because of the limited number of effective resistance genes, efforts to identify new sources of resistance are frequently focused on genetically diversified landraces. The goal of this study was to characterise the powdery mildew resistance gene in barley line 2553-3 selected from the Moroccan landrace. Phytopathological testing against a set of differential pathogen isolates revealed different pattern responses of this gene from those of other known resistance genes. F and F (2553-3 × Manchuria) mapping populations were employed to investigate resistance inheritance. Two approaches were applied for the linkage analysis: in the first approach, 22 resistant and 21 susceptible homozygous F plants genotyped by the DArTseq platform (Diversity Arrays Technology, Pty. Ltd.) were used; in the second, 94 F plants were genotyped by converted DArTseq markers and SSRs. Both analyses delineated a new resistance gene on the short arm of chromosome 2H. The authors propose MlMor as a gene symbol for newly characterized powdery mildew resistance genes in barley line 255-3-3. The results presented herein provide a good foundation for the development of closer linkage markers and MAS breeding.

Citing Articles

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References

Mascher M, Muehlbauer G, Rokhsar D, Chapman J, Schmutz J, Barry K . Anchoring and ordering NGS contig assemblies by population sequencing (POPSEQ). Plant J. 2013; 76(4):718-27. PMC: 4298792. DOI: 10.1111/tpj.12319. View

Druka A, Kudrna D, Kannangara C, von Wettstein D, Kleinhofs A . Physical and genetic mapping of barley (Hordeum vulgare) germin-like cDNAs. Proc Natl Acad Sci U S A. 2002; 99(2):850-5. PMC: 117394. DOI: 10.1073/pnas.022627999. View

You F, Huo N, Gu Y, Luo M, Ma Y, Hane D . BatchPrimer3: a high throughput web application for PCR and sequencing primer design. BMC Bioinformatics. 2008; 9:253. PMC: 2438325. DOI: 10.1186/1471-2105-9-253. View

Lucas J, Hawkins N, Fraaije B . The evolution of fungicide resistance. Adv Appl Microbiol. 2015; 90:29-92. DOI: 10.1016/bs.aambs.2014.09.001. View

Cruz V, Kilian A, Dierig D . Development of DArT marker platforms and genetic diversity assessment of the U.S. collection of the new oilseed crop lesquerella and related species. PLoS One. 2013; 8(5):e64062. PMC: 3665832. DOI: 10.1371/journal.pone.0064062. View

Schonfeld M, Ragni A, Fischbeck G, Jahoor A . RFLP mapping of three new loci for resistance genes to powdery mildew (Erysiphe graminis f. sp. hordei) in barley. Theor Appl Genet. 2013; 93(1-2):48-56. DOI: 10.1007/BF00225726. View

Aghnoum R, Marcel T, Johrde A, Pecchioni N, Schweizer P, Niks R . Basal host resistance of barley to powdery mildew: connecting quantitative trait Loci and candidate genes. Mol Plant Microbe Interact. 2009; 23(1):91-102. DOI: 10.1094/MPMI-23-1-0091. View

Mohler V, Jahoor A . Allele-specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals. Theor Appl Genet. 2013; 93(7):1078-82. DOI: 10.1007/BF00230128. View

Honsdorf N, March T, Berger B, Tester M, Pillen K . High-throughput phenotyping to detect drought tolerance QTL in wild barley introgression lines. PLoS One. 2014; 9(5):e97047. PMC: 4019662. DOI: 10.1371/journal.pone.0097047. View

10.

Pickering R, Hill A, Michel M, Timmerman-Vaughan G . The transfer of a powdery mildew resistance gene from Hordeum bulbosum L to barley (H. vulgare L.) chromosome 2 (2I). Theor Appl Genet. 2013; 91(8):1288-92. DOI: 10.1007/BF00220943. View

11.

Giese H, Holm-Jensen A, Jensen H, Jensen J . Localization of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RFLP markers. Theor Appl Genet. 2013; 85(6-7):897-900. DOI: 10.1007/BF00225035. View

12.

Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden T . Primer-BLAST: a tool to design target-specific primers for polymerase chain reaction. BMC Bioinformatics. 2012; 13:134. PMC: 3412702. DOI: 10.1186/1471-2105-13-134. View

13.

Hoseinzadeh P, Zhou R, Mascher M, Himmelbach A, Niks R, Schweizer P . High Resolution Genetic and Physical Mapping of a Major Powdery Mildew Resistance Locus in Barley. Front Plant Sci. 2019; 10:146. PMC: 6382739. DOI: 10.3389/fpls.2019.00146. View

14.

Marcel T, Aghnoum R, Durand J, Varshney R, Niks R . Dissection of the barley 2L1.0 region carrying the 'Laevigatum' quantitative resistance gene to leaf rust using near-isogenic lines (NIL) and subNIL. Mol Plant Microbe Interact. 2007; 20(12):1604-15. DOI: 10.1094/MPMI-20-12-1604. View

15.

Spies A, Korzun V, Bayles R, Rajaraman J, Himmelbach A, Hedley P . Allele mining in barley genetic resources reveals genes of race-non-specific powdery mildew resistance. Front Plant Sci. 2012; 2:113. PMC: 3355509. DOI: 10.3389/fpls.2011.00113. View

16.

Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389-402. PMC: 146917. DOI: 10.1093/nar/25.17.3389. View

17.

Comadran J, Thomas W, Van Eeuwijk F, Ceccarelli S, Grando S, Stanca A . Patterns of genetic diversity and linkage disequilibrium in a highly structured Hordeum vulgare association-mapping population for the Mediterranean basin. Theor Appl Genet. 2009; 119(1):175-87. DOI: 10.1007/s00122-009-1027-0. View

18.

Cooper L, Marquez-Cedillo L, Singh J, Sturbaum A, Zhang S, Edwards V . Mapping Ds insertions in barley using a sequence-based approach. Mol Genet Genomics. 2004; 272(2):181-93. DOI: 10.1007/s00438-004-1035-3. View

19.

Buschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A . The barley Mlo gene: a novel control element of plant pathogen resistance. Cell. 1997; 88(5):695-705. DOI: 10.1016/s0092-8674(00)81912-1. View

20.

Murray M, Thompson W . Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 1980; 8(19):4321-5. PMC: 324241. DOI: 10.1093/nar/8.19.4321. View