Genetic Characterization of Two Vancomycin-resistant Isolates in Kerman, Iran

Overview

Journal Infect Drug Resist

Publisher Dove Medical Press

Date 2019 Jul 17

PMID 31308707

Citations 6

Authors

Mahsa Ziasistani

Mohammad Reza Shakibaie

Davood Kalantar-Neyestanaki

Affiliations

Soon will be listed here.

Abstract

Aim: The aim of this study was the genetic characterization of two clinical vancomycin-resistant (VRSA) isolates.

Materials And Methods: Resistance to vancomycin was determined by phenotypic method. PCR was used for detection of (2")-Ic, (3')-IIIa, , Immune Evasion Cluster [ and ] genes and biofilm operon ABCD. On the other hand, multilocus sequence typing and typing methods were performed for the determination of clonal relationship and operon was detected and sequenced.

Results: Vancomycin-resistant strain 1 (VRSA-1) was positive for (2")-Ic, (3')-IIIa, D genes, belonging to type I; SCC type III; type t030; and ST239. However, the genetic characterization of Vancomycin-resistant strain 2 (VRSA-2) revealed the presence of various types of resistance genes (2")-Ic, (3')-IIIa, , i, relating to type I; SCC type III; type t459; and ST239. The presence of transposon Tn was determined by PCR sequencing.The Basic Local Alignment Search Tool analysis of operon in the VRSA isolates showed 99.6% sequence homology to Tn in vancomycin-resistant enterococci, indicating the operon has an enterococcal origin.

Conclusion: In conclusion, the ST239 is one of the most common clones of MRSA isolates which involved the hospital-associated infections, therefore, the emergence of VRSA isolates with ST239 increased the spread of resistance to vancomycin in the hospital settings.

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