Detection of Cell-Free Mitochondrial DNA in Cerebrospinal Fluid of Creutzfeldt-Jakob Patients

Overview

Journal Front Neurol

Specialty Neurology

Date 2019 Jul 12

PMID 31293496

Citations 1

Authors

Jie Li

Yuhan Duan

Deming Zhao

Syed Zahid Ali Shah

Wei Wu

Xixi Zhang

Mengyu Lai

Zhiling Guan

Dongming Yang

Xiaoqian Wu

Hongli Gao

Huafen Zhao

Qi Shi

Lifeng Yang

Affiliations

Soon will be listed here.

Abstract

The current diagnosis method for Creutzfeldt-Jakob disease (CJD) is post-mortem examination, so early detection of CJD has been historically problematic. Auxiliary detection of CJD based on changes in levels of components of the cerebrospinal fluid (CSF) has become a focus of research. In other neurodegenerative diseases such as Alzheimer's disease (AD), cell-free mitochondrial DNA (mtDNA) in the CSF of patients may serve as a biomarker that could facilitate early diagnosis and studies of the mechanisms underlying the disease. In this study, the cell-free mitochondrial DNA in the CSF of patients with sCJD and control patients was compared by digital droplet PCR. The cell-free mitochondrial DNA copy number in the CSF of sCJD patients was significantly increased in comparison with that of the control group, and this difference was pathologically related to CJD. Therefore, we speculate that changes in cerebrospinal fluid mitochondrial DNA copy number play an important role in the study of CJD mechanism and diagnosis.

Citing Articles

Post-mortem ventricular cerebrospinal fluid cell-free-mtDNA in neurodegenerative disease.

Lowes H, Kurzawa-Akanbi M, Pyle A, Hudson G Sci Rep. 2020; 10(1):15253.

PMID: 32943697 PMC: 7499424. DOI: 10.1038/s41598-020-72190-5.

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