Precursor RNA Processing 3 is Required for Male Fertility, and Germline Stem Cell Self-renewal and Differentiation Via Regulating Spliceosome Function in Drosophila Testes

Overview

Journal Sci Rep

Specialty Science

Date 2019 Jul 12

PMID 31292463

Citations 5

Authors

Xia Chen

Xiaojin Luan

Qianwen Zheng

Chen Qiao

Wanyin Chen

Min Wang

Yidan Yan

Bing Xie

Cong Shen

Zeyu He

Jun Zhang

Mingxi Liu

Xing Hu

Hong Li

Bo Zheng

Jie Fang

Jun Yu

Affiliations

Soon will be listed here.

Abstract

The nuclear pre-mRNA spliceosome is a large complex containing five small nuclear ribonucleoprotein particles (snRNPs) and many splicing factors. Messenger RNAs (mRNAs) are generated from pre-mRNAs by the process of RNA splicing, which is conserved in eukaryotes. Precursor RNA processing 3 (Prp3) is a U4/U6-associated snRNP whose function remains largely unknown. In the present study, using genetic manipulation of a Drosophila melanogaster testis model, we demonstrated that Prp3 is essential for male fertility in Drosophila. Prp3 deficiency in germline stem cells (GSCs) and early cyst cells resulted in abnormal structure of testes and maintenance defects of GSCs and cyst stem cells. Knockdown of Prp3 in spermatogonia and early cyst cells mediated tumor formation caused by differentiation defects. Using an in vitro assay, knockdown of Prp3 decreased proliferation and increased cell death, and controlled the spliceosome function via regulating spliceosome subunits expression in Drosophila S2 cells. We also identified two other splicing factors in the Prp complex (Prp19 and Prp8), which mimicked the phenotype of Prp3 in the Drosophila stem cell niche. Our results revealed a significant role of precursor RNA processing factors in male testes, indicating that Prp3, a key spliceosome component in the Prp complex, is essential for male fertility, and germline stem cell self-renewal and differentiation, via regulating the spliceosome function in Drosophila testes.

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