Sperm Chromatin Condensation Defects, but Neither DNA Fragmentation nor Aneuploidy, Are an Independent Predictor of Clinical Pregnancy After Intracytoplasmic Sperm Injection

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2019 Jul 11

PMID 31289980

Citations 7

Authors

C Bichara

B Berby

A Rives

F Jumeau

M Letailleur

V Setif

L Sibert

C Rondanino

Nathalie Rives

Affiliations

Soon will be listed here.

Abstract

Purpose: The impact of sperm DNA damage on intracytoplasmic sperm injection (ICSI) outcomes remains controversial. The purpose of the study was to evaluate the prognostic value of several types of sperm nuclear damage on ICSI clinical pregnancy.

Methods: Our retrospective study included a total of 132 couples who consulted for male or mixed-factor infertility that benefited from ICSI cycles from January 2006 to December 2015. All infertile males presented at least one conventional semen parameter alteration. Sperm nuclear damage was assessed using the Motile Sperm Organelle Morphological Examination for sperm head relative vacuolar area (RVA), aniline blue staining for chromatin condensation, terminal deoxynucleotidyl transferase dUTP nick-end labeling for DNA fragmentation, and fluorescence in situ hybridization for aneuploidy.

Results: Infertile males who achieved pregnancy after ICSI had fewer chromatin condensation defects than did males who did not achieve any pregnancy (15.8 ± 12.0% vs. 11.4 ± 7.9%, respectively, P = 0.0242), which remained significant in multivariate regression analysis (RR = 0.40 [0.18 to 0.86], P = 0.02). RVA, DNA fragmentation, and aneuploidy were not predictive factors of ICSI outcomes. The pregnancy rate was significantly decreased by number of progressive motile spermatozoa with normal morphology after migration (P = 0.04). In female partners, 17β estradiol of less than 2000 pg/mL on the day of ovulation induction significantly reduced the occurrence of clinical pregnancy (P = 0.04).

Conclusion: Sperm chromatin condensation defects were more frequently observed in couples with ICSI failure and should be considered a negative predictive factor for the occurrence of clinical pregnancy.

Citing Articles

First-line infertility treatment in normal or subnormal sperm: Interest of a simplified pre-IMSI test.

Sigala J, Poirey S, Robert J, Pouget O, Mura T, Huberlant S PLoS One. 2024; 19(7):e0307080.

PMID: 39008497 PMC: 11249273. DOI: 10.1371/journal.pone.0307080.

The number of retrieved oocytes is correlated to sperm DNA fragmentation when predicting pregnancy outcomes.

Zhang Z, Yu C, Chu Z, Heng B, Tong G, Xia Y Am J Transl Res. 2023; 15(5):3759-3765.

PMID: 37303625 PMC: 10251026.

Sperm Chromatin Condensation Defect Accelerates the Kinetics of Early Embryonic Development but Does Not Modify ICSI Outcome.

Jumeau F, Rives N, Lechevallier P, Boniou C, Letailleur M, Real-Lhommet A Int J Mol Sci. 2023; 24(1).

PMID: 36613835 PMC: 9820555. DOI: 10.3390/ijms24010393.

Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used.

Ribas-Maynou J, Novo S, Torres M, Salas-Huetos A, Rovira S, Antich M Biol Res. 2022; 55(1):41.

PMID: 36572948 PMC: 9791757. DOI: 10.1186/s40659-022-00409-y.

Human Sperm Morphology as a Marker of Its Nuclear Quality and Epigenetic Pattern.

Bendayan M, Caceres L, Sais E, Swierkowski-Blanchard N, Alter L, Bonnet-Garnier A Cells. 2022; 11(11).

PMID: 35681482 PMC: 9180039. DOI: 10.3390/cells11111788.

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