Use of Monoclonal Antibodies in Formol-paraffin Sections. A Study of Normal and Neoplastic Haemopoietic Tissues

Overview

Journal Virchows Arch A Pathol Anat Histopathol

Specialty Anatomy & Histology

Date 1988 Jan 1

PMID 3128918

Citations 1

Authors

T Magyarlaki

G KELENYI

Affiliations

Soon will be listed here.

Abstract

Thirteen monoclonal antibodies (the "VI-series") reactive with B- and T-lymphocytes, monocytes, granulocytes and erythrocytes were tested in sections fixed with formalin, formalin-sublimate or formalin-acetic acid. After fixation and embedding, most of the antigens were not detectable. However, VIE-G 4, a monoclonal antibody selective for glycophorin A, produced a strong reaction with erythroid cells in formalin fixed sections. The binding of the monoclonal antibodies VIM-D 5, VIM-2 and VIM-13 to myeloid, myelomonocytic and monocytic cells became either more intense or was inhibited by preincubation of the sections with pronase, trypsin, papain or neuraminidase. Following enzyme digestion, the monocyte-specific antibody VIM-13 reacted selectively with some of the germinal centre cells.

Citing Articles

Haemopoiesis in human fetal and embryonic liver. Immunohistochemical determination in B5-fixed paraffin-embedded tissues.

Timens W, Kamps W, Poppema S Virchows Arch A Pathol Anat Histopathol. 1990; 416(5):429-36.

PMID: 2107630 DOI: 10.1007/BF01605149.

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