Nutrient Composition and Safety Evaluation of Simulated Isobutanol Distillers Dried Grains with Solubles and Associated Fermentation Metabolites when Fed to Male Ross 708 Broiler Chickens (Gallus Domesticus)

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2019 Jul 9

PMID 31283767

Authors

Jason M Roper

Brenda L Smith

Jessica M Caverly Rae

Emily Huang

Carl A Walker

James L McNaughton

Alice Chen

Kathy S Reidinger

Affiliations

Soon will be listed here.

Abstract

Saccharomyces cerevisiae genetically engineered to enhance butanol production will be used in a manufacturing process similar to that of fuel ethanol production, including co-production of distillers products for animal feed. A poultry feeding trial was conducted with simulated isobutanol-derived dried distillers grains with solubles (bDDGS), comprising non-fermentable corn solids and heat-inactivated Butamax modified yeast (BMY), to determine potential health effects. Simulated dried distillers grains were produced in 2 variants: bDDGS containing 10% (B10) or 50% (B50) BMY. The BMY concentrations were selected based on a conservative estimate from ethanol-derived distillers grains (eDDGS) approximating 2.5 and 12-fold margins of exposure. The B10 and B50 DDGS were evaluated in a 42-day feeding trial using male Ross 708 broiler chickens fed diets containing eDDGS, B50 DDGS, or B10 DDGS without or with isobutanol, 2,3-butanediol, and isobutyric acid metabolites each at target concentrations of 2 (B10-2), 5 (B10-5), or 10 (B10-10) times the anticipated specification limit in the commercial product. Diets were fed (n = 50 broilers/treatment) in 3 phases: starter phase with 8% DDGS and grower and finisher phases each with 15% DDGS. No statistically significant differences or diet-related effects on mortality, clinical pathology, or organ weights, and no microscopic observations associated with consumption of diets containing B10, B50, or B10 supplemented with metabolites at any targeted exposure level were observed. A lower (P < 0.05) mean absolute bursa of Fabricius weight in the B10-10 group compared to the B10 group was considered to be within the range of biological variability. A non-significant trend toward lower weight, gains, and feed intake, and higher feed:gain ratio was observed in the B10-10 group, and was considered a non-adverse palatability effect of consuming high concentrations of metabolites. These results demonstrate that consumption of phase diets containing simulated DDGS from a novel isobutanol production process was well-tolerated.

References

Martinez-Amezcua C, Parsons C, Singh V, Srinivasan R, Murthy G . Nutritional characteristics of corn distillers dried grains with solubles as affected by the amounts of grains versus solubles and different processing techniques. Poult Sci. 2007; 86(12):2624-30. DOI: 10.3382/ps.2007-00137. View

Spiehs M, Whitney M, Shurson G . Nutrient database for distiller's dried grains with solubles produced from new ethanol plants in Minnesota and South Dakota. J Anim Sci. 2002; 80(10):2639-45. DOI: 10.2527/2002.80102639x. View

Driver J, Pesti G, Bakalli R, Edwards Jr H . Calcium requirements of the modern broiler chicken as influenced by dietary protein and age. Poult Sci. 2005; 84(10):1629-39. DOI: 10.1093/ps/84.10.1629. View

Galfi P, BOKORI J . Feeding trial in pigs with a diet containing sodium n-butyrate. Acta Vet Hung. 1990; 38(1-2):3-17. View

Shim M, Pesti G, Bakalli R, Tillman P, Payne R . Evaluation of corn distillers dried grains with solubles as an alternative ingredient for broilers. Poult Sci. 2011; 90(2):369-76. DOI: 10.3382/ps.2010-00727. View

Li P, Li D, Zhang H, Li Z, Zhao P, Zeng Z . Determination and prediction of energy values in corn distillers dried grains with solubles sources with varying oil content for growing pigs. J Anim Sci. 2015; 93(7):3458-70. DOI: 10.2527/jas.2014-8782. View

Corzo A, Schilling M, Loar 2nd R, Jackson V, Kin S, Radhakrishnan V . The effects of feeding distillers dried grains with solubles on broiler meat quality. Poult Sci. 2009; 88(2):432-9. DOI: 10.3382/ps.2008-00406. View

Stahly T, Cromwell G, Monegue H . Effects of dietary additions of 1,3-butanediol or lard for sows on survival of neonatal pigs. J Anim Sci. 1986; 63(4):1156-62. DOI: 10.2527/jas1986.6341156x. View

Turner T, Kim H, Kong I, Liu J, Zhang G, Jin Y . Engineering and Evolution of Saccharomyces cerevisiae to Produce Biofuels and Chemicals. Adv Biochem Eng Biotechnol. 2016; 162:175-215. DOI: 10.1007/10_2016_22. View

10.

SMYTH Jr H, CARPENTER C, WEIL C, POZZANI U, STRIEGEL J . Range-finding toxicity data: List VI. Am Ind Hyg Assoc J. 1962; 23:95-107. DOI: 10.1080/00028896209343211. View

11.

Rychen G, Aquilina G, Azimonti G, Bampidis V, Bastos M, Bories G . Guidance on the assessment of the safety of feed additives for the target species. EFSA J. 2020; 15(10):e05021. PMC: 7009839. DOI: 10.2903/j.efsa.2017.5021. View

12.

Nielsen D, Leonard E, Yoon S, Tseng H, Yuan C, Jones Prather K . Engineering alternative butanol production platforms in heterologous bacteria. Metab Eng. 2009; 11(4-5):262-73. DOI: 10.1016/j.ymben.2009.05.003. View

13.

Pahm A, Scherer C, Pettigrew J, Baker D, Parsons C, Stein H . Standardized amino acid digestibility in cecectomized roosters and lysine bioavailability in chicks fed distillers dried grains with solubles. Poult Sci. 2009; 88(3):571-8. DOI: 10.3382/ps.2008-00184. View

14.

Schilling K, Kayser M, Deckardt K, Kuttler K, Klimisch H . Subchronic toxicity studies of 3-methyl-1-butanol and 2-methyl-1-propanol in rats. Hum Exp Toxicol. 1998; 16(12):722-6. DOI: 10.1177/096032719701601205. View

15.

Kim Y, Mosier N, Hendrickson R, Ezeji T, Blaschek H, Dien B . Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage. Bioresour Technol. 2007; 99(12):5165-76. DOI: 10.1016/j.biortech.2007.09.028. View

16.

Fang C, Sun H, Wu J, Niu H, Feng J . Effects of sodium butyrate on growth performance, haematological and immunological characteristics of weanling piglets. J Anim Physiol Anim Nutr (Berl). 2013; 98(4):680-5. DOI: 10.1111/jpn.12122. View

17.

Meloche K, Kerr B, Billor N, Shurson G, Dozier 3rd W . Validation of prediction equations for apparent metabolizable energy of corn distillers dried grains with solubles in broiler chicks. Poult Sci. 2014; 93(6):1428-39. DOI: 10.3382/ps.2013-03712. View

18.

Generoso W, Schadeweg V, Oreb M, Boles E . Metabolic engineering of Saccharomyces cerevisiae for production of butanol isomers. Curr Opin Biotechnol. 2014; 33:1-7. DOI: 10.1016/j.copbio.2014.09.004. View

19.

Meluzzi A, Primiceri G, Giordani R, Fabris G . Determination of blood constituents reference values in broilers. Poult Sci. 1992; 71(2):337-45. DOI: 10.3382/ps.0710337. View

20.

Doud D, Holmes E, Richter H, Molitor B, Jander G, Angenent L . Metabolic engineering of for the obligate reduction of -butyrate to -butanol. Biotechnol Biofuels. 2017; 10:178. PMC: 5504763. DOI: 10.1186/s13068-017-0864-3. View