A Homeobox Transcription Factor UvHOX2 Regulates Chlamydospore Formation, Conidiogenesis, and Pathogenicity in

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Jul 9

PMID 31281290

Citations 19

Authors

Junjie Yu

Mina Yu

Tianqiao Song

Huijuan Cao

Xiayan Pan

Mingli Yong

Zhongqiang Qi

Yan Du

Rongsheng Zhang

Xiaole Yin

Yongfeng Liu

Affiliations

Soon will be listed here.

Abstract

Rice false smut fungus (teleomorph: ; anamorph: ) can generate chlamydospores and survive winter under field conditions. The chlamydospore is considered as an important infection source of the disease. However, little is known about the regulatory mechanism of the chlamydospore production. In this study, we identified a defective homeobox transcription factor (designated as UvHOX2) gene in a random insertional mutant B-766 that could not form chlamydospores. To confirm the regulatory function of UvHOX2, an mediated transformation- and CRISPR/Cas9- based targeted gene replacement method was developed. The deletion mutants completely failed to produce chlamydospores, showed reduced conidia production and decreased virulence, and was hyper-sensitive to oxidative, osmotic, and cell wall stresses. We confirmed that UvHOX2 is located in the nuclei of , and the expression of was the strongest during the early stage of chlamydospore and conidium formation. Global transcription pattern of UvHOX2 was also determined by RNA-seq in this study, and several genes that might be down-stream of UvHOX2 regulation were identified. The results will better our understanding of the molecular mechanism of chlamydospore formation in as a model fungus.

Citing Articles

Identification of Homeobox Transcription Factors in a Dimorphic Fungus and Protein-Protein Interaction Prediction of RfeB.

Pongpom M, Khamto N, Sukantamala P, Kalawil T, Wangsanut T J Fungi (Basel). 2024; 10(10).

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AfSwi6 Regulates the Stress Response, Chlamydospore Production, and Pathogenicity in the Nematode-Trapping Fungus .

Linghu S, Zhang Y, Zuo J, Mo M, Li G Microorganisms. 2024; 12(9).

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The Ras GTPase-activating protein UvGap1 orchestrates conidiogenesis and pathogenesis in the rice false smut fungus Ustilaginoidea virens.

Cao H, Gong H, Yu M, Pan X, Song T, Yu J Mol Plant Pathol. 2024; 25(3):e13448.

PMID: 38502297 PMC: 10950028. DOI: 10.1111/mpp.13448.

Regulatory functions of homeobox domain transcription factors in fungi.

Calvo A, Dabholkar A, Wyman E, Lohmar J, Cary J Appl Environ Microbiol. 2024; 90(3):e0220823.

PMID: 38421174 PMC: 10952592. DOI: 10.1128/aem.02208-23.

Transcriptomic and Metabolomic Analyses Provide Insights into the Pathogenic Mechanism of the Rice False Smut Pathogen .

Fu R, Wang J, Chen C, Liu Y, Zhao L, Lu D Int J Mol Sci. 2023; 24(13).

PMID: 37445981 PMC: 10342015. DOI: 10.3390/ijms241310805.

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