Polymorphisms in the Bovine Tumour Necrosis Factor Receptor Type Two Gene (TNF-RII) and Cell Subpopulations Naturally Infected with Bovine Leukaemia Virus

Overview

Journal J Vet Res

Publisher Sciendo

Specialty Veterinary Medicine

Date 2019 Jul 6

PMID 31276056

Citations 1

Authors

Alicja Stachura

Barbara Bojarojc-Nosowicz

Dariusz Kaczmarczyk

Ewa Kaczmarczyk

Affiliations

Soon will be listed here.

Abstract

Introduction: Numerous mutations in the bovine tumour necrosis factor receptor type two (TNF-RII) gene have been identified, but their biological consequences remain poorly understood. The aim of this study was to determine whether polymorphism in the analysed loci of the bovine TNF-RII gene is linked with the size of cell subpopulations naturally infected with bovine leukaemia virus (BLV) which serve important immune functions in the host.

Material And Methods: Samples originated from 78 cows. Polymorphisms in the studied gene were determined by PCR-RFLP and DNA sequencing by capillary electrophoresis. BLV infection was diagnosed by the immunofluorescence (IMF) technique and nested PCR. Cell subpopulations were immunophenotyped with IMF.

Results: Similar and non-significant differences in the average percentages of TNFα±, IgM+TNFα±, and CD11b+TNFα±cells infected with BLV were noted in individuals with various genotypes in the polymorphic sites g.-1646T > G and g. 16534T > C of the TNF-RII gene, and significant differences in the percentages of these subpopulations were observed between selected microsatellite genotypes (g.16512CA).

Conclusion: STR polymorphism and the number of CA dinucleotide repeats in intron 1 of the TNF-RII gene influence the frequency of TNF+, CD11b+TNF+, and IgM+TNF+ subpopulations naturally infected with BLV. Polymorphism in the gene's other two sites do not affect the size of these cell subpopulations.

Citing Articles

Cytokine TNF-α and its receptors TNFRI and TNFRII play a key role in the in vitro proliferative response of BLV infected animals.

Lendez P, Martinez-Cuesta L, Nieto Farias M, Dolcini G, Ceriani M Vet Res Commun. 2021; 45(4):431-439.

PMID: 34453235 DOI: 10.1007/s11259-021-09825-z.

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